Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102703 |
| Name | oriT_INF298|2  |
| Organism | Klebsiella pneumoniae strain INF298 isolate INF298 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_LR890427 (135798..135847 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_INF298|2
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1989 | GenBank | WP_117389463 |
| Name | traI_JMX68_RS27560_INF298|2 |
UniProt ID | _ |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190207.96 Da Isoelectric Point: 6.2061
>WP_117389463.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MLSFSQVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKRDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSENILANRIALGKIYQNSLRADVES
MGYKTVDAGKNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALNTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLGSKSRGVRDYYREGMVMERWDPETRSHARFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
IGHGWVESPGRSVSETATVFASVTQLELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTIQ
AQIRSGQLLNVPVAHGHGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAQSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTSEQVPRKEGAWAPGSSVVEFTPKQEKAIQK
AVSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHHISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEMITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGQLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKRDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSENILANRIALGKIYQNSLRADVES
MGYKTVDAGKNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALNTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLGSKSRGVRDYYREGMVMERWDPETRSHARFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
IGHGWVESPGRSVSETATVFASVTQLELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTIQ
AQIRSGQLLNVPVAHGHGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAQSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTSEQVPRKEGAWAPGSSVVEFTPKQEKAIQK
AVSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHHISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEMITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGQLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 1630 | GenBank | WP_086625007 |
| Name | traD_JMX68_RS27565_INF298|2 |
UniProt ID | _ |
| Length | 760 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 760 a.a. Molecular weight: 84895.84 Da Isoelectric Point: 4.9578
>WP_086625007.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPESVSPPA
PAEMTVTPAPVKAPPTTKRPAAEPPVLPVTPVPLLNPKAAAAAATASSAGTPAAAAGGTEQELAQQSAEQ
GQDMLPAGMNEDGVIEDMQAYDSWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNI
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPESVSPPA
PAEMTVTPAPVKAPPTTKRPAAEPPVLPVTPVPLLNPKAAAAAATASSAGTPAAAAGGTEQELAQQSAEQ
GQDMLPAGMNEDGVIEDMQAYDSWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 107486..136405
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JMX68_RS27565 | 107486..109768 | - | 2283 | WP_086625007 | type IV conjugative transfer system coupling protein TraD | virb4 |
| JMX68_RS27570 | 109895..110584 | - | 690 | WP_162881175 | hypothetical protein | - |
| JMX68_RS27575 | 110777..111508 | - | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| JMX68_RS27580 | 111701..112228 | - | 528 | WP_094067313 | conjugal transfer protein TraS | - |
| JMX68_RS27585 | 112234..115083 | - | 2850 | WP_094067297 | conjugal transfer mating-pair stabilization protein TraG | traG |
| JMX68_RS27590 | 115083..116462 | - | 1380 | WP_162881176 | conjugal transfer pilus assembly protein TraH | traH |
| JMX68_RS27595 | 116440..116883 | - | 444 | WP_094067299 | F-type conjugal transfer protein TrbF | - |
| JMX68_RS27600 | 116929..117486 | - | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| JMX68_RS27605 | 117458..117697 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| JMX68_RS27610 | 117708..118460 | - | 753 | WP_094067300 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| JMX68_RS27615 | 118481..118807 | - | 327 | WP_086625003 | hypothetical protein | - |
| JMX68_RS27620 | 118820..119068 | - | 249 | WP_004152675 | hypothetical protein | - |
| JMX68_RS27625 | 119046..119300 | - | 255 | WP_094067301 | conjugal transfer protein TrbE | - |
| JMX68_RS27630 | 119332..121287 | - | 1956 | WP_086625001 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| JMX68_RS27635 | 121346..121993 | - | 648 | WP_015632510 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| JMX68_RS27640 | 122269..123258 | - | 990 | WP_094067302 | conjugal transfer pilus assembly protein TraU | traU |
| JMX68_RS27645 | 123255..123656 | - | 402 | WP_004194979 | hypothetical protein | - |
| JMX68_RS27650 | 123691..124326 | - | 636 | WP_020325113 | type-F conjugative transfer system protein TraW | traW |
| JMX68_RS27655 | 124326..124715 | - | 390 | WP_094067303 | type-F conjugative transfer system protein TrbI | - |
| JMX68_RS27660 | 124715..127354 | - | 2640 | WP_094067304 | type IV secretion system protein TraC | virb4 |
| JMX68_RS27665 | 127426..127824 | - | 399 | WP_162881177 | hypothetical protein | - |
| JMX68_RS27670 | 127849..128253 | - | 405 | WP_094067306 | hypothetical protein | - |
| JMX68_RS27675 | 128320..128631 | - | 312 | WP_094067307 | hypothetical protein | - |
| JMX68_RS27680 | 128632..128850 | - | 219 | WP_086624994 | hypothetical protein | - |
| JMX68_RS27685 | 129174..129758 | - | 585 | WP_086624992 | type IV conjugative transfer system lipoprotein TraV | traV |
| JMX68_RS27690 | 129781..130377 | - | 597 | WP_094067308 | conjugal transfer pilus-stabilizing protein TraP | - |
| JMX68_RS27695 | 130370..131794 | - | 1425 | WP_094067309 | F-type conjugal transfer pilus assembly protein TraB | traB |
| JMX68_RS27700 | 131794..132534 | - | 741 | WP_094067310 | type-F conjugative transfer system secretin TraK | traK |
| JMX68_RS27705 | 132521..133087 | - | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| JMX68_RS27710 | 133107..133412 | - | 306 | WP_094067311 | type IV conjugative transfer system protein TraL | traL |
| JMX68_RS27715 | 133426..133794 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| JMX68_RS27720 | 133856..134062 | - | 207 | WP_171773970 | TraY domain-containing protein | - |
| JMX68_RS27725 | 134195..134914 | - | 720 | WP_159049099 | conjugal transfer protein TrbJ | - |
| JMX68_RS27730 | 135113..135529 | - | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| JMX68_RS27735 | 135920..136405 | + | 486 | WP_064172160 | transglycosylase SLT domain-containing protein | virB1 |
| JMX68_RS27740 | 136438..136767 | - | 330 | WP_094067312 | DUF5983 family protein | - |
| JMX68_RS27745 | 136800..137621 | - | 822 | WP_048276959 | DUF932 domain-containing protein | - |
| JMX68_RS27750 | 138249..138599 | - | 351 | WP_004152758 | hypothetical protein | - |
| JMX68_RS27755 | 138596..138868 | - | 273 | WP_094067335 | hypothetical protein | - |
| JMX68_RS27760 | 139562..139678 | - | 117 | WP_055313929 | type I toxin-antitoxin system Hok family toxin | - |
| JMX68_RS30695 | 139629..139778 | - | 150 | Protein_143 | FlmC family protein | - |
| JMX68_RS27765 | 139948..140322 | - | 375 | WP_094067337 | hypothetical protein | - |
| JMX68_RS27770 | 140378..140704 | - | 327 | WP_094067338 | theronine dehydrogenase | - |
Host bacterium
| ID | 3146 | GenBank | NZ_LR890427 |
| Plasmid name | INF298|2 | Incompatibility group | IncFIB |
| Plasmid size | 161308 bp | Coordinate of oriT [Strand] | 135798..135847 [+] |
| Host baterium | Klebsiella pneumoniae strain INF298 isolate INF298 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |