Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102681
• Name: oriT_pB323S2b
• Organism: Bradyrhizobium barranii subsp. barranii strain 323S2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP049701 (277204..277241 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 18..24, 29..35 (ACGTCGC..GCGACGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_pB323S2b
TCCAAGGGCGCAGTAATACGTCGCTGACGCGACGTCCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_G6321_RS53460_pB323S2b

• ID: 1979
• GenBank: WP_166354441
• Name: traA_G6321_RS53460_pB323S2b
• UniProt ID: _
• Length: 1102 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1102 a.a.
• Molecular weight: 123111.13 Da
• Isoelectric Point: 10.2256

>WP_166354441.1 Ti-type conjugative transfer relaxase TraA [Bradyrhizobium barranii]
MAITHFTPQLIGRGDGRSAVLAAAYRHCAKMEFEAEGRTVDYSNKRGMAHEEFLVPADAPAWLRTIIADR
SVAGASEAFWNKVEAFEKRADAQFAKEFIIALPVELTTEQNIALMRQFVVEQVLSRRQVADWVYHDEPGN
PHVHLMTTLRPLTEDGFGPKRVAVIGEDGQPLRMQSGKIAYRLWAGDKQEFLEQRNGWLDLQNQHLALNG
HEIRVDGRSYAERGIDVVPTTHIGVAAKAIQRRAEREGRDPNLERLRVFDENRAETLRRIQRRPEIVLDM
ISREKSVFDQRDVGKLLHRYVDDVGTFQDLMARVLQSPDALRLEGESVDFATGARAPAKYTTRELIRLEA
EMARRADHLSGSAAFTVKAQVIAAVSARHANLSVEQRAAIAHVVSGERIAAVVGRAGAGKTTMMRAAREV
WEAAGYRVVVGALAGKAAEGLEKEAAIQSRTLASWELRWKSGRDGLDDRTVFVLDEAGMVASRQMALFVE
TASKAGSKLVLVGDPEQLQPIEAGAAFRAIVERIGYAELETIYRQRQQWMRDASLDLARGRTGEALSAYR
HHGRLFGSELKAQAIDNLIADWNRDYDPSKSTLILAHLRRDVRALNDMARAKLVERGLIEEGHAFRTEDG
ERRFAAGDQIVFLKNEGSLGVKNGMIGKVVAAEPKRLVAEIGDGEGRRHVEVDQRFYRNIDHGYATTVHK
AQGATVDRVKVLATLSLDKHLTYVALTRHREDVALYYGRRSFQKAGGLIPILSQRRAKESTLDYECGALY
RDALRFAANRGLNIFKVARTLANDRLRWTVRQKQRLADVARRLRALGEKLGLLDSRKPIIRTAHKEAKPM
VAGVTTFTKSLTDTVEDRILADPAIKKQWEDVSTRFRLVFADPERAFRATNFDAMLKDPAAASSTMARLA
ASPESFGALRGKVGLLAGKSDKQVRQRAEANAPALKRDIERYLRLRAEAEHKHEAEERAIRHRVAIDIPA
LSREARRVLERVRDAIDRNDLPSALEFALADRMVKAEIDGFNKAVAERFGDRTFLSNAAREPNGQPFEKA
AAGMDPARREQLKEAWPAMRAAQQLAAGERTAQALKQAEGQRLTQSQGQTLK

Protein domains

Predicted by InterproScan.

• AAA domain (AAA_30) (382-565)

• MobA/MobL family (MobA_MobL) (17-243)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (858-954)

• Viral (Superfamily 1) RNA helicase (Viral_helicase1) (694-735)

Protein structure

No available structure.

T4CP

traG_G6321_RS53475_pB323S2b

• ID: 1619
• GenBank: WP_166354443
• Name: traG_G6321_RS53475_pB323S2b
• UniProt ID: _
• Length: 643 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 643 a.a.
• Molecular weight: 69419.19 Da
• Isoelectric Point: 9.1739

>WP_166354443.1 Ti-type conjugative transfer system protein TraG [Bradyrhizobium barranii]
MRANKIALAIVPAVLMASLCLVLTGIEAWLSEFGTTPNARLVLGRVGIALPYVVAAAIGVVFLLAAAGAT
AIRSAGWSVVFGGLLVVAIAASREIARPHSFANALLAGDRLLAYADPSTIIGATIAALVAVFGLRVAIKG
NAAFAAAAPRRIRGRRAIHGESDWMVMTEAARLFPETGGIVVGERYRVDRDAVTDRSFRADDAETWGAGG
KSPLLCFDASFGSSHGIVFAGSGGFKTTSVTVPTALKWGGGLVVLDPSYEVAPMVTGHRHGAGRQVFVLD
PRSSGVGFNVLDWIGQHGATKEEDIAAVASWIMTDNPRQGSARDDFFRASALQLLTALTADVCLSGHTEK
KDQHLRQVRANLSEPEPKLRQRLQTIYDNSESGFVKENVAPFIAMTPETFSGVYANAVKETHWLSYANYA
ALVSGASFTTAELAEGRTDVFINLDLKTLENYAGLARVVIGSLLNAIYNRNGEVTGRTLFLLDEVARLGY
LRILETARDAGRKYGITLLMLYQSIGQMREAYGGRDATSKWFESASWISFSAINDPETADYISKRCGDTT
VEVDQLSQSSQTSSSSRTRSKQLARRPLVLPHEVLRMRTDEQIIFTAGNPPLRCGRAIWFRRTDMRACVG
ENRFYRGEAGGRS

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (163-634)

Protein structure

No available structure.

Host bacterium

• ID: 3125
• GenBank: NZ_CP049701
• Plasmid name: pB323S2b
• Incompatibility group: -
• Plasmid size: 420539 bp
• Coordinate of oriT [Strand]: 277204..277241 [-]
• Host baterium: Bradyrhizobium barranii subsp. barranii strain 323S2

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrID1