Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102679 |
| Name | oriT_pSCU-204-1  |
| Organism | Escherichia coli strain SCU-204 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP053253 (157836..157924 [+], 89 nt) |
| oriT length | 89 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 89 nt
>oriT_pSCU-204-1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCATTTTTC
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCATTTTTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1618 | GenBank | WP_001289276 |
| Name | t4cp2_HHJ42_RS25965_pSCU-204-1 |
UniProt ID | _ |
| Length | 763 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 763 a.a. Molecular weight: 86941.11 Da Isoelectric Point: 6.7713
>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 114156..152459
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HHJ42_RS25745 (HHJ42_25750) | 109218..110285 | + | 1068 | WP_171274542 | type IV pilus biogenesis lipoprotein PilL | - |
| HHJ42_RS25750 (HHJ42_25755) | 110285..110722 | + | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| HHJ42_RS25755 (HHJ42_25760) | 110736..112418 | + | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| HHJ42_RS25760 (HHJ42_25765) | 112411..113706 | + | 1296 | WP_021560540 | type 4b pilus protein PilO2 | - |
| HHJ42_RS25765 (HHJ42_25770) | 113693..114145 | + | 453 | WP_001247336 | type IV pilus biogenesis protein PilP | - |
| HHJ42_RS25770 (HHJ42_25775) | 114156..115709 | + | 1554 | WP_000362202 | ATPase, T2SS/T4P/T4SS family | virB11 |
| HHJ42_RS25775 (HHJ42_25780) | 115722..116819 | + | 1098 | WP_171274543 | type II secretion system F family protein | - |
| HHJ42_RS25780 (HHJ42_25785) | 116837..117451 | + | 615 | WP_000959785 | type 4 pilus major pilin | - |
| HHJ42_RS25785 (HHJ42_25790) | 117461..118021 | + | 561 | WP_000014116 | lytic transglycosylase domain-containing protein | virB1 |
| HHJ42_RS25790 (HHJ42_25795) | 118006..118662 | + | 657 | WP_001193553 | prepilin peptidase | - |
| HHJ42_RS25795 (HHJ42_25800) | 118662..120092 | + | 1431 | Protein_138 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| HHJ42_RS25800 (HHJ42_25805) | 121374..121592 | - | 219 | WP_071527775 | shufflon protein D' | - |
| HHJ42_RS25805 (HHJ42_25810) | 121641..121727 | - | 87 | Protein_140 | shufflon protein D' | - |
| HHJ42_RS25810 (HHJ42_25815) | 121726..122880 | + | 1155 | WP_001139957 | site-specific integrase | - |
| HHJ42_RS25815 (HHJ42_25820) | 123031..123855 | + | 825 | WP_001238939 | conjugal transfer protein TraE | traE |
| HHJ42_RS25820 (HHJ42_25825) | 123940..125142 | + | 1203 | WP_000976351 | conjugal transfer protein TraF | - |
| HHJ42_RS25825 (HHJ42_25830) | 125202..125786 | + | 585 | WP_000977522 | histidine phosphatase family protein | - |
| HHJ42_RS25830 (HHJ42_25835) | 126181..126633 | + | 453 | Protein_145 | IncI1-type conjugal transfer lipoprotein TraH | - |
| HHJ42_RS25835 (HHJ42_25840) | 126634..127453 | + | 820 | Protein_146 | IncI1-type conjugal transfer lipoprotein TraI | - |
| HHJ42_RS25840 (HHJ42_25845) | 127450..128598 | + | 1149 | WP_080242523 | plasmid transfer ATPase TraJ | virB11 |
| HHJ42_RS25845 (HHJ42_25850) | 128595..128885 | + | 291 | WP_001299214 | hypothetical protein | traK |
| HHJ42_RS25850 (HHJ42_25855) | 128900..129451 | + | 552 | WP_000014583 | phospholipase D family protein | - |
| HHJ42_RS25855 (HHJ42_25860) | 129541..133306 | + | 3766 | Protein_150 | DNA primase | - |
| HHJ42_RS25860 (HHJ42_25865) | 133324..133671 | + | 348 | WP_001055900 | conjugal transfer protein | traL |
| HHJ42_RS25865 (HHJ42_25870) | 133668..134360 | + | 693 | WP_000138550 | DotI/IcmL family type IV secretion protein | traM |
| HHJ42_RS25870 (HHJ42_25875) | 134371..135354 | + | 984 | WP_001191877 | IncI1-type conjugal transfer protein TraN | traN |
| HHJ42_RS25875 (HHJ42_25880) | 135357..136646 | + | 1290 | WP_001272003 | conjugal transfer protein TraO | traO |
| HHJ42_RS25880 (HHJ42_25885) | 136646..137350 | + | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| HHJ42_RS25885 (HHJ42_25890) | 137350..137877 | + | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| HHJ42_RS25890 (HHJ42_25895) | 137928..138332 | + | 405 | WP_000086958 | IncI1-type conjugal transfer protein TraR | traR |
| HHJ42_RS25895 (HHJ42_25900) | 138396..138584 | + | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| HHJ42_RS25900 (HHJ42_25905) | 138568..139368 | + | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| HHJ42_RS25905 (HHJ42_25910) | 139458..142502 | + | 3045 | WP_001024782 | IncI1-type conjugal transfer protein TraU | traU |
| HHJ42_RS25910 (HHJ42_25915) | 142502..143116 | + | 615 | WP_000337399 | IncI1-type conjugal transfer protein TraV | traV |
| HHJ42_RS25915 (HHJ42_25920) | 143083..144285 | + | 1203 | WP_001385654 | IncI1-type conjugal transfer protein TraW | traW |
| HHJ42_RS25920 (HHJ42_25925) | 144314..144898 | + | 585 | WP_001037987 | IncI1-type conjugal transfer protein TraX | - |
| HHJ42_RS25925 (HHJ42_25930) | 144995..147163 | + | 2169 | WP_171274544 | DotA/TraY family protein | traY |
| HHJ42_RS25930 (HHJ42_25935) | 147237..147887 | + | 651 | WP_032179289 | plasmid IncI1-type surface exclusion protein ExcA | - |
| HHJ42_RS25935 (HHJ42_25940) | 147959..148168 | - | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| HHJ42_RS25940 (HHJ42_25945) | 148560..148736 | + | 177 | WP_001054897 | hypothetical protein | - |
| HHJ42_RS25945 (HHJ42_25950) | 149395..149646 | + | 252 | WP_001291964 | hypothetical protein | - |
| HHJ42_RS25950 (HHJ42_25955) | 149718..149870 | - | 153 | WP_001331364 | Hok/Gef family protein | - |
| HHJ42_RS25955 (HHJ42_25960) | 150162..151370 | + | 1209 | WP_000121273 | IncI1-type conjugal transfer protein TrbA | trbA |
| HHJ42_RS25960 (HHJ42_25965) | 151389..152459 | + | 1071 | WP_000151583 | IncI1-type conjugal transfer protein TrbB | trbB |
| HHJ42_RS25965 (HHJ42_25970) | 152452..154743 | + | 2292 | WP_001289276 | F-type conjugative transfer protein TrbC | - |
Host bacterium
| ID | 3123 | GenBank | NZ_CP053253 |
| Plasmid name | pSCU-204-1 | Incompatibility group | IncFIA |
| Plasmid size | 174260 bp | Coordinate of oriT [Strand] | 157836..157924 [+] |
| Host baterium | Escherichia coli strain SCU-204 |
Cargo genes
| Drug resistance gene | sul2, aph(3'')-Ib, aph(6)-Id |
| Virulence gene | senB |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |