Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102678
Name   oriT_FWSEC0147|unnamed4 in_silico
Organism   Escherichia coli strain FWSEC0147
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRHM01000159 (76825..76897 [+], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_FWSEC0147|unnamed4
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1978 GenBank   WP_000991386
Name   Relaxase_C9Z97_RS27115_FWSEC0147|unnamed4 insolico UniProt ID   A0A648F3C8
Length   903 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 903 a.a.        Molecular weight: 104553.93 Da        Isoelectric Point: 7.5347

>WP_000991386.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
TTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLVWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGS
VRFRDRRNGELVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAW
HNAAGITGNGHFVISRPDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure


Source ID Structure
AlphaFold DB A0A648F3C8


Auxiliary protein

ID   803 GenBank   WP_001291058
Name   WP_001291058_FWSEC0147|unnamed4 insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   1617 GenBank   WP_000077655
Name   trbC_C9Z97_RS27095_FWSEC0147|unnamed4 insolico UniProt ID   _
Length   767 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 767 a.a.        Molecular weight: 87075.22 Da        Isoelectric Point: 7.7106

>WP_000077655.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIWPLALPVCLLFTLVTSVIFTLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVM
FFATTGGGKTETTFAWLLNPLSWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMILARDLGYRLE
GTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSGGKQESRKKRF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 18236..51339

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9Z97_RS26805 (C9Z97_26845) 14306..14743 + 438 WP_000539685 type IV pilus biogenesis protein PilM -
C9Z97_RS26810 (C9Z97_26850) 14774..16393 + 1620 WP_001034998 PilN family type IVB pilus formation outer membrane protein -
C9Z97_RS26815 (C9Z97_26855) 16414..17709 + 1296 WP_000129889 type 4b pilus protein PilO2 -
C9Z97_RS26820 (C9Z97_26860) 17699..18151 + 453 WP_001112926 type IV pilus biogenesis protein PilP -
C9Z97_RS26825 (C9Z97_26865) 18236..19744 + 1509 WP_000886638 ATPase, T2SS/T4P/T4SS family virB11
C9Z97_RS26830 (C9Z97_26870) 19746..20846 + 1101 WP_000670135 type II secretion system F family protein -
C9Z97_RS26835 (C9Z97_26875) 20920..21456 + 537 WP_001011156 type 4 pilus major pilin -
C9Z97_RS26840 (C9Z97_26880) 21510..21983 + 474 WP_000870986 lytic transglycosylase domain-containing protein virB1
C9Z97_RS26845 (C9Z97_26885) 22002..22637 + 636 WP_000478596 prepilin peptidase -
C9Z97_RS26850 (C9Z97_26890) 22642..24006 + 1365 WP_001168072 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
C9Z97_RS26865 (C9Z97_26905) 24621..25790 + 1170 WP_000486835 site-specific integrase -
C9Z97_RS26870 (C9Z97_26910) 25832..26122 + 291 WP_000681898 hypothetical protein -
C9Z97_RS26875 (C9Z97_26915) 26214..26534 - 321 WP_000695052 hypothetical protein -
C9Z97_RS26880 (C9Z97_26920) 26794..27615 + 822 WP_000804090 hypothetical protein traE
C9Z97_RS26885 (C9Z97_26925) 27705..28907 + 1203 WP_000980151 conjugal transfer protein TraF -
C9Z97_RS27555 29047..29217 + 171 WP_000482367 hypothetical protein -
C9Z97_RS26890 (C9Z97_26930) 29266..29718 + 453 WP_001081198 DotD/TraH family lipoprotein -
C9Z97_RS26895 (C9Z97_26935) 29715..30551 + 837 WP_000848138 type IV secretory system conjugative DNA transfer family protein traI
C9Z97_RS26900 (C9Z97_26940) 30535..31683 + 1149 WP_000625246 plasmid transfer ATPase TraJ virB11
C9Z97_RS26905 (C9Z97_26945) 31680..31970 + 291 WP_000817799 hypothetical protein traK
C9Z97_RS26910 (C9Z97_26950) 32034..36050 + 4017 WP_021554958 LPD7 domain-containing protein -
C9Z97_RS26915 (C9Z97_26955) 36067..36417 + 351 WP_001056365 hypothetical protein traL
C9Z97_RS26920 (C9Z97_26960) 36429..37124 + 696 WP_001287261 DotI/IcmL family type IV secretion protein traM
C9Z97_RS26925 (C9Z97_26965) 37135..38109 + 975 WP_001165565 DotH/IcmK family type IV secretion protein traN
C9Z97_RS26930 (C9Z97_26970) 38113..39444 + 1332 WP_009453278 conjugal transfer protein TraO traO
C9Z97_RS26935 (C9Z97_26975) 39441..40154 + 714 WP_000787006 conjugal transfer protein TraP traP
C9Z97_RS26940 (C9Z97_26980) 40151..40681 + 531 WP_000987022 conjugal transfer protein TraQ traQ
C9Z97_RS26945 (C9Z97_26985) 40728..41126 + 399 WP_000086067 DUF6750 family protein traR
C9Z97_RS28260 (C9Z97_26990) 41183..41434 + 252 WP_001277575 hypothetical protein -
C9Z97_RS26955 (C9Z97_26995) 41349..42167 + 819 WP_072148514 conjugal transfer protein TraT traT
C9Z97_RS27560 42226..42585 + 360 WP_000379509 hypothetical protein -
C9Z97_RS26960 (C9Z97_27000) 42585..42965 + 381 WP_000690882 hypothetical protein -
C9Z97_RS26965 (C9Z97_27005) 42938..43483 - 546 WP_001369268 hypothetical protein -
C9Z97_RS26970 (C9Z97_27010) 43708..46752 + 3045 WP_001024760 hypothetical protein traU
C9Z97_RS26975 (C9Z97_27015) 46752..47369 + 618 WP_000286861 hypothetical protein traV
C9Z97_RS26980 (C9Z97_27020) 47330..48535 + 1206 WP_001189539 conjugal transfer protein TraW traW
C9Z97_RS26985 (C9Z97_27025) 48532..49101 + 570 WP_000650755 conjugal transfer protein TraX -
C9Z97_RS26990 (C9Z97_27030) 49174..51339 + 2166 WP_000691804 DotA/TraY family protein traY
C9Z97_RS26995 (C9Z97_27035) 51410..52063 + 654 WP_001098361 plasmid IncI1-type surface exclusion protein ExcA -
C9Z97_RS27000 (C9Z97_27040) 52337..53923 + 1587 WP_000004565 TnsA endonuclease N-terminal domain-containing protein -
C9Z97_RS27005 (C9Z97_27045) 54061..54213 - 153 WP_001597021 Hok/Gef family protein -
C9Z97_RS27010 (C9Z97_27050) 54546..54788 + 243 WP_000064387 hypothetical protein -

Host bacterium

ID   3122 GenBank   NZ_RRHM01000159
Plasmid name   FWSEC0147|unnamed4 Incompatibility group   IncB/O/K/Z
Plasmid size   82080 bp Coordinate of oriT [Strand]   76825..76897 [+]
Host baterium   Escherichia coli strain FWSEC0147

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -