Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102661
• Name: oriT_pC20
• Organism: Escherichia coli strain c20
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_NGBR01000046 (20889..21143 [+], 255 nt)
• oriT length: 255 nt
• IRs (inverted repeats): 153..158, 160..165 (AAAAGT..ACTTTT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 255 nt

>oriT_pC20
GGATTTAGGTTTTTTTTTAATCGCTTCACAGTTCGTTAGCAAGCTCAGTTTTTTTTGATAAAATTCTGGTCAGTTTGTTTAAAAAGTGTTACAAGTAAGGCGAATGGTTGAATGGTTAGTTTTAAGACTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

TraI_2_B6N66_RS24340_pC20

• ID: 1969
• GenBank: WP_249537831
• Name: TraI_2_B6N66_RS24340_pC20
• UniProt ID: _
• Length: 910 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 910 a.a.
• Molecular weight: 102054.08 Da
• Isoelectric Point: 4.5177

>WP_249537831.1 TraI domain-containing protein [Escherichia coli]
MLSHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (1-164)

Protein structure

No available structure.

T4CP

traD_B6N66_RS24335_pC20

• ID: 1607
• GenBank: WP_046788497
• Name: traD_B6N66_RS24335_pC20
• UniProt ID: _
• Length: 694 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 694 a.a.
• Molecular weight: 78171.95 Da
• Isoelectric Point: 8.0177

>WP_046788497.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKFH

Protein domains

Predicted by InterproScan.

• AAA-like domain (AAA_10) (199-249)

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (406-609)

Protein structure

No available structure.

Host bacterium

• ID: 3105
• GenBank: NZ_NGBR01000046
• Plasmid name: pC20
• Incompatibility group: -
• Plasmid size: 76326 bp
• Coordinate of oriT [Strand]: 20889..21143 [+]
• Host baterium: Escherichia coli strain c20

Cargo genes

• Drug resistance gene: sul1, dfrA12, aph(3')-Ia, sul3, ant(3'')-Ia, cmlA1, aadA2, sul2, aph(4)-Ia, aac(3)-IVa
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -