Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102655
Name   oriT_pR7AC in_silico
Organism   Escherichia coli strain R7AC
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LAZA01000043 (36178..36266 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pR7AC
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1966 GenBank   WP_010891268
Name   nikB_UG47_RS04045_pR7AC insolico UniProt ID   A0A632VGA6
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103977.42 Da        Isoelectric Point: 7.2753

>WP_010891268.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYIAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A632VGA6


Auxiliary protein

ID   790 GenBank   WP_001283947
Name   WP_001283947_pR7AC insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1603 GenBank   WP_001289276
Name   trbC_UG47_RS04040_pR7AC insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1370..30800

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UG47_RS03875 (UG47_03405) 65..1219 + 1155 WP_001139957 site-specific integrase -
UG47_RS03880 (UG47_03410) 1370..2194 + 825 WP_001238939 conjugal transfer protein TraE traE
UG47_RS03885 (UG47_03415) 2279..3481 + 1203 WP_000976351 conjugal transfer protein TraF -
UG47_RS03890 (UG47_03420) 3541..4125 + 585 WP_000977522 histidine phosphatase family protein -
UG47_RS03895 (UG47_03425) 4520..4978 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
UG47_RS03900 (UG47_03430) 4975..5793 + 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
UG47_RS03905 (UG47_03435) 5790..6938 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
UG47_RS03910 6935..7225 + 291 WP_001299214 hypothetical protein traK
UG47_RS03915 (UG47_03440) 7240..7791 + 552 WP_000014583 phospholipase D family protein -
UG47_RS03920 (UG47_03445) 7881..11648 + 3768 WP_001141529 LPD7 domain-containing protein -
UG47_RS03925 (UG47_03450) 11666..12013 + 348 WP_001055900 conjugal transfer protein traL
UG47_RS03930 (UG47_03455) 12010..12702 + 693 WP_000138548 DotI/IcmL family type IV secretion protein traM
UG47_RS03935 (UG47_03460) 12713..13696 + 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
UG47_RS03940 (UG47_03465) 13699..14988 + 1290 WP_001272005 conjugal transfer protein TraO traO
UG47_RS03945 (UG47_03470) 14988..15692 + 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
UG47_RS03950 (UG47_03475) 15692..16219 + 528 WP_001055569 conjugal transfer protein TraQ traQ
UG47_RS03955 (UG47_03480) 16270..16674 + 405 WP_000086957 IncI1-type conjugal transfer protein TraR traR
UG47_RS03960 (UG47_03485) 16738..16926 + 189 WP_001277255 putative conjugal transfer protein TraS -
UG47_RS03965 (UG47_03490) 16910..17710 + 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
UG47_RS03970 (UG47_03495) 17800..20844 + 3045 WP_001024780 IncI1-type conjugal transfer protein TraU traU
UG47_RS03975 (UG47_03500) 20844..21458 + 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
UG47_RS03980 (UG47_03505) 21425..22627 + 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
UG47_RS03985 (UG47_03510) 22656..23240 + 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
UG47_RS03990 (UG47_03515) 23337..25499 + 2163 WP_000698351 DotA/TraY family protein traY
UG47_RS03995 (UG47_03520) 25564..26226 + 663 WP_000653334 plasmid IncI1-type surface exclusion protein ExcA -
UG47_RS04000 (UG47_03525) 26298..26507 - 210 WP_000062603 HEAT repeat domain-containing protein -
UG47_RS31025 (UG47_03535) 26899..27075 + 177 WP_001054904 hypothetical protein -
UG47_RS32100 27140..27235 - 96 WP_000609148 DinQ-like type I toxin DqlB -
UG47_RS04020 (UG47_03545) 27736..27987 + 252 WP_001291964 hypothetical protein -
UG47_RS04025 (UG47_03550) 28059..28211 - 153 WP_001303307 Hok/Gef family protein -
UG47_RS04030 (UG47_03555) 28503..29711 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
UG47_RS04035 (UG47_03560) 29730..30800 + 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
UG47_RS04040 (UG47_03565) 30793..33084 + 2292 WP_001289276 F-type conjugative transfer protein TrbC -

Host bacterium

ID   3099 GenBank   NZ_LAZA01000043
Plasmid name   pR7AC Incompatibility group   IncI1
Plasmid size   91075 bp Coordinate of oriT [Strand]   36178..36266 [+]
Host baterium   Escherichia coli strain R7AC

Cargo genes

Drug resistance gene   blaCMY-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -