oriTDB

Detailed information of oriT

oriT
Relaxase
Auxiliary protein
T4CP
T4SS
Host bacterium
Cargo genes

oriT

The information of the oriT region

oriTDB ID	102651
Name	oriT_ECOR31\|unnamed1
Organism	Escherichia coli strain ECOR31
Sequence Completeness	-
*NCBI accession of oriT* (coordinates [strand])**	NZ_MIIL01000002 (82720..82805 [+], 86 nt)
oriT length	86 nt
IRs (inverted repeats)	61..68, 73..80 (TTGGTGGT..ACCACCAA) 27..34, 37..44 (GCAAAAAC..GTTTTTGC) 8..14, 20..26 (TGATTTA..TAAATCA)
*Location of nic* site**	53..54
Conserved sequence flanking the nic site	TGTGTGGTGC
Note	Predicted by oriTfinder 2.0

oriT sequence

Download Length: 86 nt

                        >oriT_ECOR31|unnamed1
AATTACATGATTTAAAACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT                      

Visualization of oriT structure

oriT secondary structure

Predicted by RNAfold.

Download structure file

Relaxase

traI_BHF03_RS25155_ECOR31|unnamed1

ID	1964	GenBank	WP_000948354
Name	traI_BHF03_RS25155_ECOR31\|unnamed1	UniProt ID	A0AA36L3I8
Length	1756 a.a.	PDB ID
Note	Predicted by oriTfinder 2.0

Relaxase protein sequence

Download Length: 1756 a.a. Molecular weight: 191683.22 Da Isoelectric Point: 5.6912

          >WP_000948354.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]

MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ

DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV

MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA

LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT

LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE

NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS

DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR

RQLQEGMVFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW

QGGEQRPATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT

MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS

SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL

ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI

KARAGETSLETAISLQKTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA

QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI

LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT

QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA

ADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA

QQKAMLKGETFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM

VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT

LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI

TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL

EPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG

IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE

GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP

DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQIERDMVRDLQKE

KTLGGD

Protein domains

Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

AAA domain (AAA_30)

(968-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

Protein structure

No available structure.

ID	787	GenBank	WP_000089263
Name	WP_000089263_ECOR31\|unnamed1	UniProt ID	A0A3U3XJL9
Length	75 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

Auxiliary protein sequence

Download Length: 75 a.a. Molecular weight: 8541.74 Da Isoelectric Point: 8.6796

                        >WP_000089263.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]

MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVRLRDHLNRFPDFYNLDAIKEGAEETDS

IIKDL

Protein domains

Predicted by InterproScan.

TraY domain (TraY)

(14-60)

Protein structure

Source	ID	Structure
AlphaFold DB	A0A3U3XJL9

ID	788	GenBank	WP_001354030
Name	WP_001354030_ECOR31\|unnamed1	UniProt ID	A0A734M5H3
Length	127 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

Auxiliary protein sequence

Download Length: 127 a.a. Molecular weight: 14508.47 Da Isoelectric Point: 4.7116

                        >WP_001354030.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]

MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLELGLRVYEAQMERKESAFNQTEFNKLL

LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDEE

Protein domains

Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)

Protein structure

Source	ID	Structure
AlphaFold DB	A0A734M5H3

T4CP

traD_BHF03_RS25160_ECOR31|unnamed1
traC_BHF03_RS25255_ECOR31|unnamed1

ID	1599	GenBank	WP_000009331
Name	traD_BHF03_RS25160_ECOR31\|unnamed1	UniProt ID	_
Length	738 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

T4CP protein sequence

Download Length: 738 a.a. Molecular weight: 83900.57 Da Isoelectric Point: 5.1644

                        >WP_000009331.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]

MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLVLWVKISWQTFINGCIYWWCTSLEGMR

DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ

QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR

QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG

SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA

IRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV

WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA

EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL

KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ

PQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYE

AWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF

Protein domains

Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

Protein structure

No available structure.

ID	1600	GenBank	WP_000069777
Name	traC_BHF03_RS25255_ECOR31\|unnamed1	UniProt ID	_
Length	876 a.a.	PDB ID	_
Note	Predicted by oriTfinder 2.0

T4CP protein sequence

Download Length: 876 a.a. Molecular weight: 99195.86 Da Isoelectric Point: 6.1126

                        >WP_000069777.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]

MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI

PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA

ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIV

GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF

LWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG

ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK

GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSG

AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS

VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY

TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGW

RLLDFKNRKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY

NQLYPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEG

LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

Protein domains

Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(290-447)

TraG P-loop domain (P-loop_TraG)

(468-764)

TraC protein (TraC_F_IV)

(39-277)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 54872..83373

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHF03_RS25160 (BHF03_25225)	54872..57088	-	2217	WP_000009331	type IV conjugative transfer system coupling protein TraD	virb4
BHF03_RS25165 (BHF03_25230)	57139..57876	-	738	WP_000199905	hypothetical protein	-
BHF03_RS25170 (BHF03_25235)	58079..58810	-	732	WP_000782451	conjugal transfer complement resistance protein TraT	-
BHF03_RS25175 (BHF03_25240)	58859..59344	-	486	WP_000605870	hypothetical protein	-
BHF03_RS25180 (BHF03_25245)	59360..62182	-	2823	WP_001007039	conjugal transfer mating-pair stabilization protein TraG	traG
BHF03_RS25185 (BHF03_25250)	62179..63552	-	1374	WP_000944331	conjugal transfer pilus assembly protein TraH	traH
BHF03_RS25190 (BHF03_25255)	63539..63931	-	393	WP_000660699	F-type conjugal transfer protein TrbF	-
BHF03_RS25195 (BHF03_25260)	63912..64259	-	348	WP_001309242	P-type conjugative transfer protein TrbJ	-
BHF03_RS25200 (BHF03_25265)	64189..64734	-	546	WP_000059831	type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB	traF
BHF03_RS25205 (BHF03_25270)	64721..65005	-	285	WP_000624194	type-F conjugative transfer system pilin chaperone TraQ	-
BHF03_RS25210 (BHF03_25275)	65124..65468	-	345	WP_000556796	conjugal transfer protein TrbA	-
BHF03_RS25215 (BHF03_25280)	65482..66225	-	744	WP_001030371	type-F conjugative transfer system pilin assembly protein TraF	traF
BHF03_RS25220 (BHF03_25285)	66218..66475	-	258	WP_000864353	conjugal transfer protein TrbE	-
BHF03_RS25225 (BHF03_25290)	66502..68352	-	1851	WP_000821856	type-F conjugative transfer system mating-pair stabilization protein TraN	traN
BHF03_RS25230 (BHF03_25295)	68349..68987	-	639	WP_001080256	type-F conjugative transfer system pilin assembly protein TrbC	trbC
BHF03_RS25235 (BHF03_25300)	68996..69301	-	306	WP_000224416	hypothetical protein	-
BHF03_RS25240 (BHF03_25305)	69331..70323	-	993	WP_000830838	conjugal transfer pilus assembly protein TraU	traU
BHF03_RS25245 (BHF03_25310)	70320..70952	-	633	WP_001203728	type-F conjugative transfer system protein TraW	traW
BHF03_RS25250 (BHF03_25315)	70949..71335	-	387	WP_000214084	type-F conjugative transfer system protein TrbI	-
BHF03_RS25255 (BHF03_25320)	71332..73962	-	2631	WP_000069777	type IV secretion system protein TraC	virb4
BHF03_RS29215 (BHF03_25325)	74088..74435	-	348	WP_000836682	hypothetical protein	-
BHF03_RS25265 (BHF03_25330)	74463..74681	-	219	WP_000556745	hypothetical protein	-
BHF03_RS25270 (BHF03_25335)	74761..75234	-	474	WP_000549568	hypothetical protein	-
BHF03_RS25275 (BHF03_25340)	75227..75640	-	414	WP_000549589	hypothetical protein	-
BHF03_RS28435	75633..75854	-	222	WP_001278683	conjugal transfer protein TraR	-
BHF03_RS25280 (BHF03_25345)	75989..76504	-	516	WP_000809881	type IV conjugative transfer system lipoprotein TraV	traV
BHF03_RS25285 (BHF03_25350)	76501..76821	-	321	WP_001057307	conjugal transfer protein TrbD	-
BHF03_RS25290 (BHF03_25355)	76808..77374	-	567	WP_000896599	conjugal transfer pilus-stabilizing protein TraP	-
BHF03_RS25295 (BHF03_25360)	77364..78815	-	1452	WP_000146675	F-type conjugal transfer pilus assembly protein TraB	traB
BHF03_RS25300 (BHF03_25365)	78815..79543	-	729	WP_001230772	type-F conjugative transfer system secretin TraK	traK
BHF03_RS25305 (BHF03_25370)	79530..80096	-	567	WP_000399780	type IV conjugative transfer system protein TraE	traE
BHF03_RS25310 (BHF03_25375)	80118..80429	-	312	WP_000012113	type IV conjugative transfer system protein TraL	traL
BHF03_RS25315 (BHF03_25380)	80444..80803	-	360	WP_001098992	type IV conjugative transfer system pilin TraA	-
BHF03_RS25320 (BHF03_25385)	80836..81063	-	228	WP_000089263	conjugal transfer relaxosome protein TraY	-
BHF03_RS25325 (BHF03_25390)	81200..81871	-	672	WP_000283561	conjugal transfer transcriptional regulator TraJ	-
BHF03_RS25330 (BHF03_25395)	82065..82448	-	384	WP_001354030	conjugal transfer relaxosome DNA-binding protein TraM	-
BHF03_RS25335 (BHF03_25400)	82771..83373	+	603	WP_000243713	transglycosylase SLT domain-containing protein	virB1
BHF03_RS25340 (BHF03_25405)	83670..84491	-	822	WP_001234445	DUF932 domain-containing protein	-
BHF03_RS25345 (BHF03_25410)	84602..84898	-	297	WP_001272251	hypothetical protein	-
BHF03_RS29530 (BHF03_25415)	85198..85494	+	297	Protein_108	hypothetical protein	-
BHF03_RS25355 (BHF03_25420)	85813..85938	-	126	WP_001372321	type I toxin-antitoxin system Hok family toxin	-
BHF03_RS29935	85880..86029	-	150	Protein_110	plasmid maintenance protein Mok	-
BHF03_RS29360 (BHF03_25425)	86051..86230	+	180	WP_001309233	hypothetical protein	-
BHF03_RS25365 (BHF03_25430)	86208..86970	-	763	Protein_112	plasmid SOS inhibition protein A	-
BHF03_RS25370 (BHF03_25435)	86967..87401	-	435	WP_000845953	conjugation system SOS inhibitor PsiB	-
BHF03_RS25375 (BHF03_25440)	87470..88057	-	588	Protein_114	hypothetical protein	-

Host bacterium

ID	3095	GenBank	NZ_MIIL01000002
Plasmid name	ECOR31\|unnamed1	Incompatibility group	IncFII
Plasmid size	101407 bp	Coordinate of oriT [Strand]	82720..82805 [+]
Host baterium	Escherichia coli strain ECOR31

Cargo genes

Drug resistance gene	tet(B), aph(3')-Ia, sul1, aadA7
Virulence gene	-
Metal resistance gene	merR, merT, merP, merC, merA, merD, merE
Degradation gene	-
Symbiosis gene	-
Anti-CRISPR	-