Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102648
Name   oriT_FWSEC0146|unnamed2 in_silico
Organism   Escherichia coli strain FWSEC0146
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRHL01000171 (4729..4817 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_FWSEC0146|unnamed2
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1962 GenBank   WP_042966220
Name   nikB_C9Z96_RS26485_FWSEC0146|unnamed2 insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103942.37 Da        Isoelectric Point: 7.2642

>WP_042966220.1 MULTISPECIES: IncI1-type relaxase NikB [Escherichia]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIALQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVSDWLSLHRRLAEDGLYLSQMDGK
YLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure



No available structure.




Auxiliary protein

ID   786 GenBank   WP_001283947
Name   WP_001283947_FWSEC0146|unnamed2 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1597 GenBank   WP_077877339
Name   trbC_C9Z96_RS26490_FWSEC0146|unnamed2 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86942.09 Da        Isoelectric Point: 6.6404

>WP_077877339.1 F-type conjugative transfer protein TrbC [Escherichia coli]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGIDLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10195..46338

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9Z96_RS26490 (C9Z96_26545) 7911..10202 - 2292 WP_077877339 F-type conjugative transfer protein TrbC -
C9Z96_RS26495 (C9Z96_26550) 10195..11265 - 1071 WP_000151577 IncI1-type conjugal transfer protein TrbB trbB
C9Z96_RS26500 (C9Z96_26555) 11284..12492 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
C9Z96_RS26505 (C9Z96_26560) 12754..13662 - 909 WP_000055084 hypothetical protein -
C9Z96_RS28880 14294..14389 + 96 WP_001303310 DinQ-like type I toxin DqlB -
C9Z96_RS28065 14454..14630 - 177 WP_001054904 hypothetical protein -
C9Z96_RS26510 (C9Z96_26565) 15022..15231 + 210 WP_000062603 HEAT repeat domain-containing protein -
C9Z96_RS26515 (C9Z96_26570) 15303..15953 - 651 WP_001178506 plasmid IncI1-type surface exclusion protein ExcA -
C9Z96_RS26520 (C9Z96_26575) 16027..18195 - 2169 WP_000698357 DotA/TraY family protein traY
C9Z96_RS26525 (C9Z96_26580) 18292..18876 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
C9Z96_RS26530 (C9Z96_26585) 18905..20107 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
C9Z96_RS26535 (C9Z96_26590) 20074..20688 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
C9Z96_RS26540 (C9Z96_26595) 20688..23732 - 3045 WP_042966216 IncI1-type conjugal transfer protein TraU traU
C9Z96_RS26545 (C9Z96_26600) 23822..24622 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
C9Z96_RS26550 (C9Z96_26605) 24606..24794 - 189 WP_001277255 putative conjugal transfer protein TraS -
C9Z96_RS26555 (C9Z96_26610) 24858..25262 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
C9Z96_RS26560 (C9Z96_26615) 25313..25840 - 528 WP_001055569 conjugal transfer protein TraQ traQ
C9Z96_RS26565 (C9Z96_26620) 25840..26544 - 705 WP_042966215 IncI1-type conjugal transfer protein TraP traP
C9Z96_RS26570 (C9Z96_26625) 26544..27833 - 1290 WP_001271997 conjugal transfer protein TraO traO
C9Z96_RS26575 (C9Z96_26630) 27836..28819 - 984 WP_136776081 IncI1-type conjugal transfer protein TraN traN
C9Z96_RS26580 (C9Z96_26635) 28830..29522 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
C9Z96_RS26585 (C9Z96_26640) 29519..29866 - 348 WP_001055900 conjugal transfer protein traL
C9Z96_RS26590 (C9Z96_26645) 29884..33651 - 3768 WP_001141542 LPD7 domain-containing protein -
C9Z96_RS26595 (C9Z96_26650) 33741..34292 - 552 WP_000014584 phospholipase D family protein -
C9Z96_RS26600 (C9Z96_26655) 34307..34597 - 291 WP_001372180 hypothetical protein traK
C9Z96_RS26605 (C9Z96_26660) 34594..35742 - 1149 WP_136776083 plasmid transfer ATPase TraJ virB11
C9Z96_RS26610 (C9Z96_26665) 35739..36557 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
C9Z96_RS26615 (C9Z96_26670) 36554..37012 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
C9Z96_RS26620 (C9Z96_26675) 37407..37991 - 585 WP_000977522 histidine phosphatase family protein -
C9Z96_RS26625 (C9Z96_26680) 38051..39253 - 1203 WP_000976348 conjugal transfer protein TraF -
C9Z96_RS26630 (C9Z96_26685) 39339..40163 - 825 WP_136776085 conjugal transfer protein TraE traE
C9Z96_RS26635 (C9Z96_26690) 40371..40496 + 126 Protein_42 RNA-guided endonuclease TnpB family protein -
C9Z96_RS26640 (C9Z96_26695) 40553..41845 - 1293 WP_001350014 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
C9Z96_RS26645 (C9Z96_26700) 41845..42501 - 657 WP_001193549 A24 family peptidase -
C9Z96_RS26650 (C9Z96_26705) 42486..43046 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
C9Z96_RS26655 (C9Z96_26710) 43056..43670 - 615 WP_000908226 type 4 pilus major pilin -
C9Z96_RS26660 (C9Z96_26715) 43687..44772 - 1086 WP_001208802 type II secretion system F family protein -
C9Z96_RS26665 (C9Z96_26720) 44785..46338 - 1554 WP_000362206 ATPase, T2SS/T4P/T4SS family virB11
C9Z96_RS26670 (C9Z96_26725) 46349..46801 - 453 WP_001247337 type IV pilus biogenesis protein PilP -
C9Z96_RS26675 (C9Z96_26730) 46788..48083 - 1296 WP_000752780 type 4b pilus protein PilO2 -
C9Z96_RS26680 (C9Z96_26735) 48076..49758 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
C9Z96_RS26685 (C9Z96_26740) 49772..50209 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
C9Z96_RS26690 (C9Z96_26745) 50209..51276 - 1068 WP_032072201 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   3092 GenBank   NZ_RRHL01000171
Plasmid name   FWSEC0146|unnamed2 Incompatibility group   IncI1
Plasmid size   75942 bp Coordinate of oriT [Strand]   4729..4817 [-]
Host baterium   Escherichia coli strain FWSEC0146

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -