Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102637
Name   oriT_46-4|unnamed in_silico
Organism   Escherichia coli strain 46-4
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LFJX01000048 (69297..69385 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_46-4|unnamed
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1960 GenBank   WP_049589785
Name   nikB_ACM16_RS23360_46-4|unnamed insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103938.34 Da        Isoelectric Point: 7.3426

>WP_049589785.1 IncI1-type relaxase NikB [Escherichia coli]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSLQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure



No available structure.




Auxiliary protein

ID   785 GenBank   WP_001283947
Name   WP_001283947_46-4|unnamed insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1588 GenBank   WP_023210866
Name   trbC_ACM16_RS23365_46-4|unnamed insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86911.08 Da        Isoelectric Point: 6.7713

>WP_023210866.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 74763..105678

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACM16_RS23365 (ACM16_23455) 72479..74770 - 2292 WP_023210866 F-type conjugative transfer protein TrbC -
ACM16_RS23370 (ACM16_23460) 74763..75833 - 1071 WP_000151571 IncI1-type conjugal transfer protein TrbB trbB
ACM16_RS23375 (ACM16_23465) 75852..77060 - 1209 WP_001383960 IncI1-type conjugal transfer protein TrbA trbA
ACM16_RS23380 (ACM16_23470) 77278..78264 - 987 WP_001257838 hypothetical protein -
ACM16_RS23385 (ACM16_23475) 78833..78985 + 153 WP_001331364 Hok/Gef family protein -
ACM16_RS23390 (ACM16_23480) 79057..79308 - 252 WP_001291962 hypothetical protein -
ACM16_RS26655 (ACM16_23485) 79807..79902 + 96 WP_001303310 DinQ-like type I toxin DqlB -
ACM16_RS26260 (ACM16_23490) 79967..80143 - 177 WP_001054898 hypothetical protein -
ACM16_RS23410 (ACM16_23500) 80535..80744 + 210 WP_000062603 HEAT repeat domain-containing protein -
ACM16_RS23415 (ACM16_23505) 80816..81478 - 663 WP_023210864 plasmid IncI1-type surface exclusion protein ExcA -
ACM16_RS23420 (ACM16_23510) 81543..83711 - 2169 WP_001690625 DotA/TraY family protein traY
ACM16_RS23425 (ACM16_23515) 83808..84392 - 585 WP_001037998 conjugal transfer protein TraX -
ACM16_RS23430 (ACM16_23520) 84421..85623 - 1203 WP_001690627 IncI1-type conjugal transfer protein TraW traW
ACM16_RS23435 (ACM16_23525) 85590..86204 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
ACM16_RS23440 (ACM16_23530) 86204..89248 - 3045 WP_001690626 IncI1-type conjugal transfer protein TraU traU
ACM16_RS23445 (ACM16_23535) 89338..90138 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
ACM16_RS23450 (ACM16_23540) 90122..90310 - 189 WP_001277255 putative conjugal transfer protein TraS -
ACM16_RS23455 (ACM16_23545) 90374..90778 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
ACM16_RS23460 (ACM16_23550) 90829..91356 - 528 WP_049589784 conjugal transfer protein TraQ traQ
ACM16_RS23465 (ACM16_23555) 91356..92060 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
ACM16_RS23470 (ACM16_23560) 92060..93349 - 1290 WP_001271994 conjugal transfer protein TraO traO
ACM16_RS23475 (ACM16_23565) 93352..94335 - 984 WP_001191879 IncI1-type conjugal transfer protein TraN traN
ACM16_RS23480 (ACM16_23570) 94346..95038 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
ACM16_RS23485 (ACM16_23575) 95035..95382 - 348 WP_001055900 conjugal transfer protein traL
ACM16_RS23490 (ACM16_23580) 95400..99167 - 3768 WP_001141534 LPD7 domain-containing protein -
ACM16_RS23495 (ACM16_23585) 99257..99808 - 552 WP_000014584 phospholipase D family protein -
ACM16_RS25420 99823..100113 - 291 WP_001330823 hypothetical protein traK
ACM16_RS23500 (ACM16_23590) 100110..101258 - 1149 WP_001024975 plasmid transfer ATPase TraJ virB11
ACM16_RS23505 (ACM16_23595) 101255..102073 - 819 WP_000866663 IncI1-type conjugal transfer lipoprotein TraI traI
ACM16_RS23510 (ACM16_23600) 102070..102528 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
ACM16_RS23515 (ACM16_23605) 102923..103507 - 585 WP_000977525 histidine phosphatase family protein -
ACM16_RS23520 (ACM16_23610) 103567..104769 - 1203 WP_000976353 conjugal transfer protein TraF -
ACM16_RS23525 (ACM16_23615) 104854..105678 - 825 WP_001238939 conjugal transfer protein TraE traE
ACM16_RS23530 (ACM16_23620) 105829..106983 - 1155 WP_001139957 site-specific integrase -
ACM16_RS26550 106982..107266 + 285 WP_187642572 pilus assembly protein PilV -
ACM16_RS25445 108504..108752 + 249 WP_001349157 hypothetical protein -
ACM16_RS25450 108749..109023 - 275 WP_075287002 hypothetical protein -

Host bacterium

ID   3081 GenBank   NZ_LFJX01000048
Plasmid name   46-4|unnamed Incompatibility group   IncI1
Plasmid size   109023 bp Coordinate of oriT [Strand]   69297..69385 [-]
Host baterium   Escherichia coli strain 46-4

Cargo genes

Drug resistance gene   tet(A)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -