Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102623
Name   oriT_73-89|unnamed contig0099 in_silico
Organism   Escherichia coli strain 73-89
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LFJY01000099 (13308..13396 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_73-89|unnamed contig0099
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1954 GenBank   WP_049589785
Name   nikB_ACM17_RS24825_73-89|unnamed contig0099 insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103938.34 Da        Isoelectric Point: 7.3426

>WP_049589785.1 IncI1-type relaxase NikB [Escherichia coli]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSLQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure



No available structure.




Auxiliary protein

ID   780 GenBank   WP_001283947
Name   WP_001283947_73-89|unnamed contig0099 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1582 GenBank   WP_023210866
Name   trbC_ACM17_RS24830_73-89|unnamed contig0099 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86911.08 Da        Isoelectric Point: 6.7713

>WP_023210866.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 18774..49689

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ACM17_RS24830 (ACM17_24945) 16490..18781 - 2292 WP_023210866 F-type conjugative transfer protein TrbC -
ACM17_RS24835 (ACM17_24950) 18774..19844 - 1071 WP_000151571 IncI1-type conjugal transfer protein TrbB trbB
ACM17_RS24840 (ACM17_24955) 19863..21071 - 1209 WP_001383960 IncI1-type conjugal transfer protein TrbA trbA
ACM17_RS24845 (ACM17_24960) 21289..22275 - 987 WP_001257838 hypothetical protein -
ACM17_RS24850 (ACM17_24965) 22844..22996 + 153 WP_001331364 Hok/Gef family protein -
ACM17_RS24855 (ACM17_24970) 23068..23319 - 252 WP_001291962 hypothetical protein -
ACM17_RS30175 (ACM17_24975) 23818..23913 + 96 WP_001303310 DinQ-like type I toxin DqlB -
ACM17_RS29465 (ACM17_24980) 23978..24154 - 177 WP_001054898 hypothetical protein -
ACM17_RS24875 (ACM17_24990) 24546..24755 + 210 WP_000062603 HEAT repeat domain-containing protein -
ACM17_RS24880 (ACM17_24995) 24827..25489 - 663 WP_023210864 plasmid IncI1-type surface exclusion protein ExcA -
ACM17_RS24885 (ACM17_25000) 25554..27722 - 2169 WP_001690625 DotA/TraY family protein traY
ACM17_RS24890 (ACM17_25005) 27819..28403 - 585 WP_001037998 conjugal transfer protein TraX -
ACM17_RS24895 (ACM17_25010) 28432..29634 - 1203 WP_001690627 IncI1-type conjugal transfer protein TraW traW
ACM17_RS24900 (ACM17_25015) 29601..30215 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
ACM17_RS24905 (ACM17_25020) 30215..33259 - 3045 WP_001690626 IncI1-type conjugal transfer protein TraU traU
ACM17_RS24910 (ACM17_25025) 33349..34149 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
ACM17_RS24915 (ACM17_25030) 34133..34321 - 189 WP_001277255 putative conjugal transfer protein TraS -
ACM17_RS24920 (ACM17_25035) 34385..34789 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
ACM17_RS24925 (ACM17_25040) 34840..35367 - 528 WP_049589784 conjugal transfer protein TraQ traQ
ACM17_RS24930 (ACM17_25045) 35367..36071 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
ACM17_RS24935 (ACM17_25050) 36071..37360 - 1290 WP_001271994 conjugal transfer protein TraO traO
ACM17_RS24940 (ACM17_25055) 37363..38346 - 984 WP_001191879 IncI1-type conjugal transfer protein TraN traN
ACM17_RS24945 (ACM17_25060) 38357..39049 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
ACM17_RS24950 (ACM17_25065) 39046..39393 - 348 WP_001055900 conjugal transfer protein traL
ACM17_RS24955 (ACM17_25070) 39411..43178 - 3768 WP_001141534 LPD7 domain-containing protein -
ACM17_RS24960 (ACM17_25075) 43268..43819 - 552 WP_000014584 phospholipase D family protein -
ACM17_RS28045 43834..44124 - 291 WP_001330823 hypothetical protein traK
ACM17_RS24965 (ACM17_25080) 44121..45269 - 1149 WP_001024975 plasmid transfer ATPase TraJ virB11
ACM17_RS24970 (ACM17_25085) 45266..46084 - 819 WP_000866663 IncI1-type conjugal transfer lipoprotein TraI traI
ACM17_RS24975 (ACM17_25090) 46081..46539 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
ACM17_RS24980 (ACM17_25095) 46934..47518 - 585 WP_000977525 histidine phosphatase family protein -
ACM17_RS24985 (ACM17_25100) 47578..48780 - 1203 WP_000976353 conjugal transfer protein TraF -
ACM17_RS24990 (ACM17_25105) 48865..49689 - 825 WP_001238939 conjugal transfer protein TraE traE
ACM17_RS24995 (ACM17_25110) 49840..50994 - 1155 WP_001139957 site-specific integrase -

Host bacterium

ID   3067 GenBank   NZ_LFJY01000099
Plasmid name   73-89|unnamed contig0099 Incompatibility group   -
Plasmid size   51115 bp Coordinate of oriT [Strand]   13308..13396 [-]
Host baterium   Escherichia coli strain 73-89

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -