Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102622 |
| Name | oriT_HMLN-1|unnamed  |
| Organism | Escherichia coli strain HMLN-1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_LFKC01000048 (22484..22586 [+], 103 nt) |
| oriT length | 103 nt |
| IRs (inverted repeats) | 64..70, 73..79 (CCACCGC..GCGGTGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 103 nt
>oriT_HMLN-1|unnamed
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCCGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGCTTGCGGTGGGCCTACTTTACATATCCTGCCCGG
AGCCTCGCAGAGCAGGATTCCCGTTGAGCAGCCCCGCTGCGAATAAGGGAAGGTAAAGTTGGACCACCGCTTGCGGTGGGCCTACTTTACATATCCTGCCCGG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1953 | GenBank | WP_049595649 |
| Name | Relaxase_ACM21_RS23900_HMLN-1|unnamed |
UniProt ID | _ |
| Length | 867 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 867 a.a. Molecular weight: 96484.68 Da Isoelectric Point: 10.5091
>WP_049595649.1 TraI/MobA(P) family conjugative relaxase [Escherichia coli]
MKNIKKSSFSGLADYITNPQSKQERVGVIKVTNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVINAIEESVCQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKFGLEVDNHMPTRSASEKRAKDMEKHSGIQSLLSWVKTECAEQLLNAHSWQELHQAMQQNGLELRQK
GNGFVIAGPDGLAVKASSIDRSLSRAALEKKLGSFEPSILDTKPSRPSPEAIKQRFGSAAHVKRPGDRPP
PMRENRQVNLGDVPVMQINSGKRYEGKPLDTQGANTTLLYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRTGRLKRSAIKLLKGAGVNKRYLYSLVSKALGAELERTNAIYKKDRQAAYDIHKRRTWADWLQHKAS
AGDDEALKILRQRNAQAARKEANLVHGVKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGQRLDISR
EAGDDGLHAALLMAKHRYGNQLTVNGSFDFKKRIVAVAAAREINVTFADPELEKLRQILTKKAAREREYI
HIPFEQRGKARQAGAMWDTEKKSWFVGPYSTRDRIENFIRMNEEKDNEQRRERNSRTEGTDSGRERRGTG
NASAADESRFSGRPTADAGTVYADGRGRGRAGAGDGITAGAGQPGGGNNARAAERGSKPVSEREHRKPNV
ARPGRQPPPFAKNRLRGMSELSMVRIAGGSEVLLPGNVPDKLEHQATERVDQLRRNIPGAGGINTPEQVT
QSLTGAQKYVAEREEKRLKGFDIPKHRRYNSTDKGQYEYAGQRKSGNETLALLKRDDEIVVMTVSDYTAR
RLSSIKVGESVTLTPSGSVRKAKGRGR
MKNIKKSSFSGLADYITNPQSKQERVGVIKVTNCQSQDVQDAVTEVNITQRLNTRSESDKTYHLVISFRP
GEKPSEDVINAIEESVCQSLGFGDHQRLSAVHHDTDNLHIHVAINKINPDTLTIHNPYNDYRVLAESCAY
LEKKFGLEVDNHMPTRSASEKRAKDMEKHSGIQSLLSWVKTECAEQLLNAHSWQELHQAMQQNGLELRQK
GNGFVIAGPDGLAVKASSIDRSLSRAALEKKLGSFEPSILDTKPSRPSPEAIKQRFGSAAHVKRPGDRPP
PMRENRQVNLGDVPVMQINSGKRYEGKPLDTQGANTTLLYGRYQSEQQRLTAARNEALRLAKVKRDRLIE
NAKRTGRLKRSAIKLLKGAGVNKRYLYSLVSKALGAELERTNAIYKKDRQAAYDIHKRRTWADWLQHKAS
AGDDEALKILRQRNAQAARKEANLVHGVKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDGGQRLDISR
EAGDDGLHAALLMAKHRYGNQLTVNGSFDFKKRIVAVAAAREINVTFADPELEKLRQILTKKAAREREYI
HIPFEQRGKARQAGAMWDTEKKSWFVGPYSTRDRIENFIRMNEEKDNEQRRERNSRTEGTDSGRERRGTG
NASAADESRFSGRPTADAGTVYADGRGRGRAGAGDGITAGAGQPGGGNNARAAERGSKPVSEREHRKPNV
ARPGRQPPPFAKNRLRGMSELSMVRIAGGSEVLLPGNVPDKLEHQATERVDQLRRNIPGAGGINTPEQVT
QSLTGAQKYVAEREEKRLKGFDIPKHRRYNSTDKGQYEYAGQRKSGNETLALLKRDDEIVVMTVSDYTAR
RLSSIKVGESVTLTPSGSVRKAKGRGR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 1581 | GenBank | WP_049595648 |
| Name | t4cp2_ACM21_RS23895_HMLN-1|unnamed |
UniProt ID | _ |
| Length | 647 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 647 a.a. Molecular weight: 71469.60 Da Isoelectric Point: 8.1384
>WP_049595648.1 type IV secretory system conjugative DNA transfer family protein [Escherichia coli]
MRKPNNAVGPQVRSKNKAVKGKLVPAMALTSLAAGVQAATQYFAYSFNYQELLGPNFNHIYAPWSYFTWY
SAWHDRLPDAFFAAGSVGTMVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKQFA
KNICLKFEPAAANGSVSWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNEGTPATLPYVDAIMADPERGAGELWMEMTQYGHVNGENHPVVGSAGRDMMDRPEEEAGSVLSTAK
SYLALYRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
DNKLTLWQRFKQAVGLSVEGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAVLGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVAEPKTTDR
IRAAAANDDASDKAIAI
MRKPNNAVGPQVRSKNKAVKGKLVPAMALTSLAAGVQAATQYFAYSFNYQELLGPNFNHIYAPWSYFTWY
SAWHDRLPDAFFAAGSVGTMVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKQFA
KNICLKFEPAAANGSVSWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNEGTPATLPYVDAIMADPERGAGELWMEMTQYGHVNGENHPVVGSAGRDMMDRPEEEAGSVLSTAK
SYLALYRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
DNKLTLWQRFKQAVGLSVEGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAVLGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVAEPKTTDR
IRAAAANDDASDKAIAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 42786..53153
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACM21_RS24020 (ACM21_24075) | 38221..38796 | - | 576 | WP_049595670 | hypothetical protein | - |
| ACM21_RS24025 (ACM21_24080) | 38793..39041 | - | 249 | WP_170317912 | antirestriction protein ArdR | - |
| ACM21_RS24030 (ACM21_24085) | 39044..39352 | - | 309 | WP_049595672 | HigA family addiction module antitoxin | - |
| ACM21_RS24035 (ACM21_24090) | 39362..39661 | - | 300 | WP_049595673 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| ACM21_RS24040 (ACM21_24095) | 40726..41685 | - | 960 | WP_248797688 | plasmid replication initiator TrfA | - |
| ACM21_RS24045 (ACM21_24100) | 41709..42068 | - | 360 | WP_049595674 | single-stranded DNA-binding protein | - |
| ACM21_RS24050 (ACM21_24105) | 42176..42589 | + | 414 | WP_049595675 | transcriptional regulator | - |
| ACM21_RS24055 (ACM21_24110) | 42786..43742 | + | 957 | WP_049595676 | P-type conjugative transfer ATPase TrbB | virB11 |
| ACM21_RS24060 (ACM21_24115) | 43842..44225 | + | 384 | WP_049595677 | TrbC/VirB2 family protein | virB2 |
| ACM21_RS24065 (ACM21_24120) | 44228..44542 | + | 315 | WP_049595678 | conjugal transfer protein TrbD | virB3 |
| ACM21_RS24070 (ACM21_24125) | 44539..47076 | + | 2538 | WP_049595679 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| ACM21_RS24075 (ACM21_24130) | 47073..47780 | + | 708 | WP_049595680 | VirB8/TrbF family protein | virB8 |
| ACM21_RS24080 (ACM21_24135) | 47797..48687 | + | 891 | WP_049595681 | P-type conjugative transfer protein TrbG | virB9 |
| ACM21_RS24085 (ACM21_24140) | 48690..49136 | + | 447 | WP_224771947 | conjugal transfer protein TrbH | - |
| ACM21_RS24090 (ACM21_24145) | 49140..50564 | + | 1425 | WP_049595683 | TrbI/VirB10 family protein | virB10 |
| ACM21_RS24095 (ACM21_24150) | 50576..51346 | + | 771 | WP_049595684 | P-type conjugative transfer protein TrbJ | virB5 |
| ACM21_RS24100 (ACM21_24155) | 51410..51583 | + | 174 | WP_049595685 | entry exclusion lipoprotein TrbK | - |
| ACM21_RS24105 (ACM21_24160) | 51588..53153 | + | 1566 | WP_049595686 | P-type conjugative transfer protein TrbL | virB6 |
| ACM21_RS24110 (ACM21_24165) | 53164..53469 | + | 306 | WP_049595687 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| ACM21_RS24115 (ACM21_24170) | 53548..54333 | + | 786 | WP_049595697 | conjugal transfer protein | - |
| ACM21_RS24120 (ACM21_24175) | 54352..55038 | + | 687 | WP_049595688 | TraX family protein | - |
| ACM21_RS24125 (ACM21_24180) | 55031..55357 | - | 327 | WP_049595689 | hypothetical protein | - |
Host bacterium
| ID | 3066 | GenBank | NZ_LFKC01000048 |
| Plasmid name | HMLN-1|unnamed | Incompatibility group | IncP1 |
| Plasmid size | 55844 bp | Coordinate of oriT [Strand] | 22484..22586 [+] |
| Host baterium | Escherichia coli strain HMLN-1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |