Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102610
Name   oriT_B86|unnamed scaffold_12 in_silico
Organism   Klebsiella pneumoniae strain B86
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LJCD01000013 (149602..149651 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_B86|unnamed scaffold_12
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1948 GenBank   WP_057729326
Name   traI_AN412_RS21585_B86|unnamed scaffold_12 insolico UniProt ID   A0A6G9HZM5
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190782.72 Da        Isoelectric Point: 6.1403

>WP_057729326.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELLEAAGPDASLKSRDVAALDTRKSKEAIDPAENMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGIK
IGHGWVESPGRSVSETAMVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERYISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVLGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLTGDDRP
WNPGAITGGRIWAEPAPVAPVPQTGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(970-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

TrwC relaxase (TrwC)

(11-283)

DNA helicase TraI (TraI_C)

(1432-1554)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)


  Protein structure



No available structure.




T4CP

ID   1575 GenBank   WP_016831058
Name   traD_AN412_RS21590_B86|unnamed scaffold_12 insolico UniProt ID   _
Length   760 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 760 a.a.        Molecular weight: 84900.95 Da        Isoelectric Point: 5.0541

>WP_016831058.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSPPA
PAEMTVSPAPVKAPPTTKRPAAEPPVLPVTPIPLLKPKAAAAAATASSAGTPAAAAGGTEQELAQQSAEQ
GQDMLPAGMNEDGVIEDMQAYAAWLADDQTQRDMQRREEVNINHNHRHDEQDDVEIGGNI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 120184..150209

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN412_RS21590 (AN412_21660) 120184..122466 - 2283 WP_016831058 type IV conjugative transfer system coupling protein TraD virb4
AN412_RS32065 122593..123282 - 690 WP_072159541 hypothetical protein -
AN412_RS21600 (AN412_21670) 123475..124206 - 732 WP_016831056 conjugal transfer complement resistance protein TraT -
AN412_RS29955 124457..125059 - 603 WP_071604857 hypothetical protein -
AN412_RS21605 (AN412_21675) 125082..127904 - 2823 WP_020325096 conjugal transfer mating-pair stabilization protein TraG traG
AN412_RS21610 (AN412_21680) 127904..129283 - 1380 WP_072159542 conjugal transfer pilus assembly protein TraH traH
AN412_RS21615 (AN412_21685) 129261..129704 - 444 WP_016831053 F-type conjugal transfer protein TrbF -
AN412_RS21620 (AN412_21690) 129750..130307 - 558 WP_020325119 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
AN412_RS21625 (AN412_21695) 130279..130518 - 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
AN412_RS21630 (AN412_21700) 130529..131281 - 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
AN412_RS21635 (AN412_21705) 131302..131628 - 327 WP_016831051 hypothetical protein -
AN412_RS21640 (AN412_21710) 131641..131889 - 249 WP_004152675 hypothetical protein -
AN412_RS21645 (AN412_21715) 131867..132121 - 255 WP_015065558 conjugal transfer protein TrbE -
AN412_RS21650 (AN412_21720) 132153..134138 - 1986 WP_020325104 type-F conjugative transfer system mating-pair stabilization protein TraN traN
AN412_RS21655 (AN412_21725) 134197..134844 - 648 WP_016831049 type-F conjugative transfer system pilin assembly protein TrbC trbC
AN412_RS32070 134923..135099 - 177 WP_162866177 hypothetical protein -
AN412_RS21660 (AN412_21730) 135120..136109 - 990 WP_015632509 conjugal transfer pilus assembly protein TraU traU
AN412_RS21665 (AN412_21735) 136106..136507 - 402 WP_020325101 hypothetical protein -
AN412_RS21670 (AN412_21740) 136542..137177 - 636 WP_020325113 type-F conjugative transfer system protein TraW traW
AN412_RS21675 (AN412_21745) 137177..137566 - 390 WP_004167468 type-F conjugative transfer system protein TrbI -
AN412_RS21680 (AN412_21750) 137566..140205 - 2640 WP_016831045 type IV secretion system protein TraC virb4
AN412_RS21685 (AN412_21755) 140277..140675 - 399 WP_023158008 hypothetical protein -
AN412_RS32400 (AN412_21760) 140683..140901 - 219 WP_072196435 hypothetical protein -
AN412_RS32405 140915..141073 - 159 WP_223372464 hypothetical protein -
AN412_RS21695 (AN412_21765) 141116..141520 - 405 WP_016831042 hypothetical protein -
AN412_RS21700 (AN412_21770) 141587..141898 - 312 WP_016831041 hypothetical protein -
AN412_RS21705 (AN412_21775) 141899..142117 - 219 WP_014386199 hypothetical protein -
AN412_RS30760 (AN412_21780) 142141..142430 - 290 Protein_151 hypothetical protein -
AN412_RS21715 (AN412_21785) 142435..142845 - 411 WP_032433731 hypothetical protein -
AN412_RS21720 (AN412_21790) 142977..143561 - 585 WP_014386196 type IV conjugative transfer system lipoprotein TraV traV
AN412_RS31280 143584..144180 - 597 Protein_154 conjugal transfer pilus-stabilizing protein TraP -
AN412_RS21735 (AN412_21805) 144173..145597 - 1425 WP_020325105 F-type conjugal transfer pilus assembly protein TraB traB
AN412_RS21740 (AN412_21810) 145597..146337 - 741 WP_014386193 type-F conjugative transfer system secretin TraK traK
AN412_RS21745 (AN412_21815) 146324..146890 - 567 WP_016831035 type IV conjugative transfer system protein TraE traE
AN412_RS21750 (AN412_21820) 146910..147215 - 306 WP_004144424 type IV conjugative transfer system protein TraL traL
AN412_RS21755 (AN412_21825) 147229..147597 - 369 WP_004194426 type IV conjugative transfer system pilin TraA -
AN412_RS21760 (AN412_21830) 147659..147865 - 207 WP_171773970 TraY domain-containing protein -
AN412_RS21765 (AN412_21835) 147998..148717 - 720 WP_072159543 conjugal transfer protein TrbJ -
AN412_RS21770 (AN412_21840) 148917..149333 - 417 WP_072145360 conjugal transfer relaxosome DNA-binding protein TraM -
AN412_RS21775 (AN412_21845) 149724..150209 + 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
AN412_RS29965 150242..150571 - 330 WP_011977736 DUF5983 family protein -
AN412_RS21780 (AN412_21850) 150604..151425 - 822 WP_004182076 DUF932 domain-containing protein -

Host bacterium

ID   3054 GenBank   NZ_LJCD01000013
Plasmid name   B86|unnamed scaffold_12 Incompatibility group   IncFIB
Plasmid size   151810 bp Coordinate of oriT [Strand]   149602..149651 [+]
Host baterium   Klebsiella pneumoniae strain B86

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsC, arsB, arsR, arsH, fecD, fecE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -