Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102609
Name   oriT1_B86|unnamed scaffold_11 in_silico
Organism   Klebsiella pneumoniae strain B86
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LJCD01000012 (56651..56716 [-], 66 nt)
oriT length   66 nt
IRs (inverted repeats)      25..32, 35..42  (TAACCGGC..GCCGGTTA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 66 nt

>oriT1_B86|unnamed scaffold_11
CGAATAAGGGACAGTGAAGATAGATAACCGGCTTGCCGGTTAGCTAACTTCACCCATCCTGCCCGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1947 GenBank   WP_057729321
Name   mobH_AN412_RS20630_B86|unnamed scaffold_11 insolico UniProt ID   _
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109609.91 Da        Isoelectric Point: 4.8956

>WP_057729321.1 MobH family relaxase [Klebsiella pneumoniae]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   1573 GenBank   WP_000178856
Name   traD_AN412_RS20635_B86|unnamed scaffold_11 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   1574 GenBank   WP_000637386
Name   traC_AN412_RS20710_B86|unnamed scaffold_11 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 84378..109569

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN412_RS20620 (AN412_20695) 80403..80636 - 234 WP_001191890 hypothetical protein -
AN412_RS20625 (AN412_20700) 80618..81235 - 618 WP_001249395 hypothetical protein -
AN412_RS20630 (AN412_20705) 81403..84381 + 2979 WP_057729321 MobH family relaxase -
AN412_RS20635 (AN412_20710) 84378..86243 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
AN412_RS20640 (AN412_20715) 86293..86838 + 546 WP_000228720 hypothetical protein -
AN412_RS20645 (AN412_20720) 86795..87424 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
AN412_RS20650 (AN412_20725) 87434..87880 + 447 WP_000122506 hypothetical protein -
AN412_RS20655 (AN412_20730) 87890..88267 + 378 WP_000869261 hypothetical protein -
AN412_RS20660 (AN412_20735) 88267..88929 + 663 WP_001231463 hypothetical protein -
AN412_RS32045 89110..89253 + 144 WP_001275801 hypothetical protein -
AN412_RS20665 (AN412_20740) 89265..89630 + 366 WP_001052531 hypothetical protein -
AN412_RS20670 (AN412_20745) 89775..90056 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
AN412_RS20675 (AN412_20750) 90053..90679 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
AN412_RS20680 (AN412_20755) 90663..91580 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
AN412_RS20685 (AN412_20760) 91580..92893 + 1314 WP_024131605 TraB/VirB10 family protein traB
AN412_RS20690 (AN412_20765) 92890..93468 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
AN412_RS20695 (AN412_20770) 93472..93864 + 393 WP_000479535 TraA family conjugative transfer protein -
AN412_RS20700 (AN412_20775) 94065..99596 + 5532 WP_000606833 Ig-like domain-containing protein -
AN412_RS20705 (AN412_20780) 99745..100452 + 708 WP_001259347 DsbC family protein -
AN412_RS20710 (AN412_20785) 100449..102896 + 2448 WP_000637386 type IV secretion system protein TraC virb4
AN412_RS20715 (AN412_20790) 102911..103228 + 318 WP_000351984 hypothetical protein -
AN412_RS20720 (AN412_20795) 103225..103755 + 531 WP_001010738 S26 family signal peptidase -
AN412_RS20725 (AN412_20800) 103718..104983 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
AN412_RS20730 (AN412_20805) 104980..105651 + 672 WP_001337754 EAL domain-containing protein -
AN412_RS20735 (AN412_20810) 105651..106655 + 1005 WP_043940352 TraU family protein traU
AN412_RS20740 (AN412_20815) 106771..109569 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
AN412_RS20745 (AN412_20820) 109608..110468 - 861 WP_000709517 hypothetical protein -
AN412_RS20750 (AN412_20825) 110591..111232 - 642 WP_000796664 hypothetical protein -
AN412_RS20755 (AN412_20830) 111526..111846 + 321 WP_000547566 hypothetical protein -
AN412_RS20760 (AN412_20835) 112150..112335 + 186 WP_001186917 hypothetical protein -
AN412_RS20765 (AN412_20840) 112555..113523 + 969 WP_000085162 AAA family ATPase -
AN412_RS20770 (AN412_20845) 113534..114442 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   3053 GenBank   NZ_LJCD01000012
Plasmid name   B86|unnamed scaffold_11 Incompatibility group   IncA/C2
Plasmid size   152848 bp Coordinate of oriT [Strand]   56651..56716 [-]; 86294..86444 [+]
Host baterium   Klebsiella pneumoniae strain B86

Cargo genes

Drug resistance gene   sul2, blaOXA-2
Virulence gene   -
Metal resistance gene   arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -