Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102602
Name   oriT_S2_VPH_Chula|unnamed1 in_silico
Organism   Salmonella enterica strain S2_VPH_Chula
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JASBCL010000002 (113263..113516 [+], 254 nt)
oriT length   254 nt
IRs (inverted repeats)      152..157, 159..164  (AAAAGT..ACTTTT)
 121..129, 134..142  (TTAAGGCTT..AAGCCTTAA)
 50..55, 59..64  (AATTTT..AAAATT)
Location of nic site      77..78
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 254 nt

>oriT_S2_VPH_Chula|unnamed1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGAAGAAATTTTGATAAAATTCTGGTCAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1945 GenBank   WP_000980119
Name   mobH_QAU02_RS22910_S2_VPH_Chula|unnamed1 insolico UniProt ID   A0A0H3VVI8
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118035.42 Da        Isoelectric Point: 4.6054

>WP_000980119.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDAITKITDT
VETAVLKVNDLGRSTASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDATMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEEEDEEHEIIDYTDFTQLSVSRPEVG
SCATSSSVHNEKLLPEPSELPELNREQNADPQGTIERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFATTESDQNGEFSLISEGDVTELEFVEIASVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A0H3VVI8


T4CP

ID   1572 GenBank   WP_001284076
Name   traD_QAU02_RS22905_S2_VPH_Chula|unnamed1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78137.93 Da        Isoelectric Point: 8.0177

>WP_001284076.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANETLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-249)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 49028..58235

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QAU02_RS22560 (QAU02_22560) 49028..51709 - 2682 WP_000387412 TraC family protein virb4
QAU02_RS22565 (QAU02_22565) 51718..52668 - 951 WP_001022587 IncHI-type conjugal transfer lipoprotein TrhV traV
QAU02_RS22570 (QAU02_22570) 52678..53430 - 753 WP_183079335 protein-disulfide isomerase HtdT -
QAU02_RS22575 (QAU02_22575) 53549..54031 - 483 WP_000377633 hypothetical protein -
QAU02_RS22580 (QAU02_22580) 54039..55394 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
QAU02_RS22585 (QAU02_22585) 55384..55845 - 462 WP_000521240 plasmid transfer protein HtdO -
QAU02_RS22590 (QAU02_22590) 55847..57118 - 1272 WP_000592090 type-F conjugative transfer system secretin TraK traK
QAU02_RS22595 (QAU02_22595) 57118..57906 - 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
QAU02_RS22600 (QAU02_22600) 57918..58235 - 318 WP_000043356 type IV conjugative transfer system protein TraL traL
QAU02_RS22605 (QAU02_22605) 58286..58639 - 354 WP_000423602 hypothetical protein -
QAU02_RS22610 (QAU02_22610) 59788..60054 - 267 Protein_54 protein RepA -
QAU02_RS22615 (QAU02_22615) 60147..61169 + 1023 WP_000255946 IS21-like element IS100 family transposase -
QAU02_RS22620 (QAU02_22620) 61166..61948 + 783 WP_001317493 IS21-like element IS100kyp family helper ATPase IstB -
QAU02_RS22625 (QAU02_22625) 61987..62619 + 633 WP_225519953 hypothetical protein -

Host bacterium

ID   3046 GenBank   NZ_JASBCL010000002
Plasmid name   S2_VPH_Chula|unnamed1 Incompatibility group   IncN
Plasmid size   293619 bp Coordinate of oriT [Strand]   113263..113516 [+]
Host baterium   Salmonella enterica strain S2_VPH_Chula

Cargo genes

Drug resistance gene   tet(M), blaTEM-1B, blaCTX-M-55, aac(3)-IId, dfrA12, aadA2, qacE, sul1, aph(3'')-Ib, aph(6)-Id, tet(A), floR, tet(X4)
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, pcoE, pcoC, pcoB, pcoA, silP, silA, silB, silF, silC, silR, silS, silE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -