Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102594
• Name: oriT_p5_UKK-0547
• Organism: Acinetobacter pittii strain UKK-0547
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_MDIK02000005 (7229..7280 [+], 52 nt)
• oriT length: 52 nt
• IRs (inverted repeats): 36..41, 45..50 (ACTCAA..TTGAGT) 7..14, 18..25 (GACTTTGC..GCAAAGTC)
• Location of nic site: 32..33
• Conserved sequence flanking the nic site: GTGAGTATAC
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 52 nt

>oriT_p5_UKK-0547
GGTCACGACTTTGCGAAGCAAAGTCTAGTGAGTATACTCAAGCATTGAGTGG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_BFR92_RS20210_p5_UKK-0547

• ID: 1941
• GenBank: WP_068540121
• Name: mobV_BFR92_RS20210_p5_UKK-0547
• UniProt ID: A0A9X5M447
• Length: 339 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 339 a.a.
• Molecular weight: 37834.15 Da
• Isoelectric Point: 10.3195

>WP_068540121.1 MobV family relaxase [Acinetobacter pittii]
MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHWAASSTDEAMGRLRELLPEKRRKDAV
LAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLS
AKEFIGNKAQMTRDQTTFAAAVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAP
QGLAEKLGISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALG
PLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-185)

Protein structure

No available structure.

Host bacterium

• ID: 3038
• GenBank: NZ_MDIK02000005
• Plasmid name: p5_UKK-0547
• Incompatibility group: -
• Plasmid size: 8804 bp
• Coordinate of oriT [Strand]: 7229..7280 [+]
• Host baterium: Acinetobacter pittii strain UKK-0547

Cargo genes

• Drug resistance gene: aph(3')-Ia
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -