Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102592 |
| Name | oriT_pU120  |
| Organism | Aromatoleum buckelii strain U120 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_WTVH02000001 (38975..39073 [-], 99 nt) |
| oriT length | 99 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 61..62 |
| Conserved sequence flanking the nic site |
TCCTGCCCGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 99 nt
>oriT_pU120
GAATAAGGGATAGCGAAGGTAGATAACCGGCTCCGCTGGTTAGCTAACTTCGCCCATCCTGCCCGCCTTTCGGCGTTCATTACCCCAGGGAAAGTCTAC
GAATAAGGGATAGCGAAGGTAGATAACCGGCTCCGCTGGTTAGCTAACTTCGCCCATCCTGCCCGCCTTTCGGCGTTCATTACCCCAGGGAAAGTCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1939 | GenBank | WP_169200211 |
| Name | Relaxase_GO608_RS00205_pU120 |
UniProt ID | _ |
| Length | 740 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 740 a.a. Molecular weight: 81823.39 Da Isoelectric Point: 10.5543
>WP_169200211.1 TraI/MobA(P) family conjugative relaxase [Aromatoleum buckelii]
MIAKHVPMGSLAKSDFAELVEYITDEQGKTERLGRVTATHCEAGTLQAVIGEVLATQRQNTRAAGDKTYH
LLVSFRPGEHPSADTLKVIEERICAGLGYGEHQRVSAVHHDTDNLHLHIAINKIHPERHTMHEPFQSYRR
LAELCAVLERDCGLERDNHQSRRSVAEGRAADMERHAGVESLVGWVRRECLEEMRGAQSWAELHQVMRKN
GLELRERGNGLVVEAADGTRVKASTIARELSKPGLEARLGPFEPSPDRLAEPKIRRQYEKSPIGLRVNTV
ELYARYKDEQQTLTAARTDSLAKARRRKDRAVEGAKRANRLWRATIKVVDSKGVGKKALYSQAGSALRDQ
LAQIHKEYANEREKLYQGFQRRAWADWLKQEALQGNADALAALRGRAAAQGLKGNTVAGQGATKSERAHV
QDNITKKGTIIFRIGQSAVRDDGDKLQVSREATREGVQAALRLAMERYGSQITVNGTPDFKARIIRTAVD
SQLPITFADPALELRRQQLLSTEKAHERAARTERGRTDRRRLGGAGSSAAADDNLARTGAGAGRGGSDDR
GGAGMDPPAHVGRKPNVGRIGRVPPPQSQHRLRGMSELGVVRIAGGSEVLLPRDVPRHVEQQRAQSDHAL
RRDAVGVGRLKPAQQAAAEEYLAEREDKRLKYFDISKNSLYNENARGFLFAGLRHFEGRTLALLRRDDAD
VVMVLPIDHATARRLSRVAVGDPVTVTPRGSIQTSKGRSR
MIAKHVPMGSLAKSDFAELVEYITDEQGKTERLGRVTATHCEAGTLQAVIGEVLATQRQNTRAAGDKTYH
LLVSFRPGEHPSADTLKVIEERICAGLGYGEHQRVSAVHHDTDNLHLHIAINKIHPERHTMHEPFQSYRR
LAELCAVLERDCGLERDNHQSRRSVAEGRAADMERHAGVESLVGWVRRECLEEMRGAQSWAELHQVMRKN
GLELRERGNGLVVEAADGTRVKASTIARELSKPGLEARLGPFEPSPDRLAEPKIRRQYEKSPIGLRVNTV
ELYARYKDEQQTLTAARTDSLAKARRRKDRAVEGAKRANRLWRATIKVVDSKGVGKKALYSQAGSALRDQ
LAQIHKEYANEREKLYQGFQRRAWADWLKQEALQGNADALAALRGRAAAQGLKGNTVAGQGATKSERAHV
QDNITKKGTIIFRIGQSAVRDDGDKLQVSREATREGVQAALRLAMERYGSQITVNGTPDFKARIIRTAVD
SQLPITFADPALELRRQQLLSTEKAHERAARTERGRTDRRRLGGAGSSAAADDNLARTGAGAGRGGSDDR
GGAGMDPPAHVGRKPNVGRIGRVPPPQSQHRLRGMSELGVVRIAGGSEVLLPRDVPRHVEQQRAQSDHAL
RRDAVGVGRLKPAQQAAAEEYLAEREDKRLKYFDISKNSLYNENARGFLFAGLRHFEGRTLALLRRDDAD
VVMVLPIDHATARRLSRVAVGDPVTVTPRGSIQTSKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 1568 | GenBank | WP_169200210 |
| Name | t4cp2_GO608_RS00210_pU120 |
UniProt ID | _ |
| Length | 634 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 634 a.a. Molecular weight: 69752.98 Da Isoelectric Point: 7.8334
>WP_169200210.1 type IV secretory system conjugative DNA transfer family protein [Aromatoleum buckelii]
MKIKMDNAVGPQVRTKKGAGGKLLPVLGAVSLGAGLQSATQFFAHTFDYQANLGVHFNHVYAPWSILEWA
SKWYSHYPNEIMQAGSIGMMISTVGLLGVAVAKVVSSNSSKANEYLHGSARWAGKKDIQTAGLLPRPRRL
LDVVTGKDAAPSTGVYVGGWEDKEGEFHYLRHNGPEHILTYAPTRSGKGVGLVVPTLLSWGDSALVTDLK
GELWAMTAGWRKKHAKNKVLRFEPATANGSVCWNPLDEIRMGTEHEVGDVQNLATLIVDPDGKGLGSHWQ
KTAFALLVGVILHALYKAKDDDTTATLPSVDAMLASPDRDIGELWMEMATYGHVAGQNHHAIGSAARDMM
DRPEEEAGSVLSTAKSFLALYRDPVVARNVSRSEFCIRDLMHSDEPVTLYIVTQPIDKDRLRPLVRVMVN
MIVRLLADKMDFDNGRPVAHYKHRLLMMLDEFPSLGKLQILQESLAFVAGYGIKCYLICQDINQLKSRET
GYGPDETITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTFQEVQRPLLT
PDECLRMPGPRKNADGNIEEPGDMVVYVAGYPAIYGKQPLYFKDPIFLARAAVPAPKVSDRLVQFAEAGE
RITI
MKIKMDNAVGPQVRTKKGAGGKLLPVLGAVSLGAGLQSATQFFAHTFDYQANLGVHFNHVYAPWSILEWA
SKWYSHYPNEIMQAGSIGMMISTVGLLGVAVAKVVSSNSSKANEYLHGSARWAGKKDIQTAGLLPRPRRL
LDVVTGKDAAPSTGVYVGGWEDKEGEFHYLRHNGPEHILTYAPTRSGKGVGLVVPTLLSWGDSALVTDLK
GELWAMTAGWRKKHAKNKVLRFEPATANGSVCWNPLDEIRMGTEHEVGDVQNLATLIVDPDGKGLGSHWQ
KTAFALLVGVILHALYKAKDDDTTATLPSVDAMLASPDRDIGELWMEMATYGHVAGQNHHAIGSAARDMM
DRPEEEAGSVLSTAKSFLALYRDPVVARNVSRSEFCIRDLMHSDEPVTLYIVTQPIDKDRLRPLVRVMVN
MIVRLLADKMDFDNGRPVAHYKHRLLMMLDEFPSLGKLQILQESLAFVAGYGIKCYLICQDINQLKSRET
GYGPDETITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTFQEVQRPLLT
PDECLRMPGPRKNADGNIEEPGDMVVYVAGYPAIYGKQPLYFKDPIFLARAAVPAPKVSDRLVQFAEAGE
RITI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 1569 | GenBank | WP_169199871 |
| Name | t4cp2_GO608_RS00490_pU120 |
UniProt ID | _ |
| Length | 845 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 845 a.a. Molecular weight: 93992.91 Da Isoelectric Point: 6.2929
>WP_169199871.1 VirB4 family type IV secretion/conjugal transfer ATPase [Aromatoleum buckelii]
MIEAIAITIAGVGTVLLLILFARIRQVDAELQLKKHRSKDAGLADLLNYASLVDDGVIVGKNGSFMAAWL
YRGDDNASSTDEQREMVSFRLNQALAGLGSGWMVHVDAVRRPAPNYSAKGSSHFRDAVSAAIDNERRRLF
EGLGTMYEGYFVLTLTWFPPLLAQRKFVELMFDDDAIAPDRQTRTMGLIEQFKRELTAIENRLSSAVKLM
RMRGQKVVNEDGSTVTHDDFLSWLQFCLTGRHHPVLLPSNPVYLDAVIGGQELWGGVVPRIGRNFVQVVA
IEGFPLESTPGLLTALGELPCEYRWSSRFIFMDPHEAVKHLDKFRKKWRQKVRGFFDQVFNTHTGPVDQD
ALSMVGDAEAAIAEVNSDLVAVGYFTGVVILMDENRDKLEESARLVEKSINRLGFAARIETINTLDAFLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGHADAPCPLYPPLSPALMHCVTHGATPFRLNLHVRDLGH
TFMFGPTGAGKSTHLALIAAQLRRYPGMTLYAFDKGMSLYPLTKAVGGLHFSVAADDDRLAFCPLQFLDS
KGDRAWAMEWIDTILALNGVETTPAQRNEIGHAIMSMHRSGARTLSELSVTIQDEAIREAIRQYTVDGTM
GHLLDAEEDGLSLADFTTFEIEELMDLGEKYALPVLLYLFRRIERSLKGQPAVIILDEAWLMLGHPAFRA
KIREWLKVLRKANCLVLMATQSLSDVANSGILDVIVESTATKIFLPNVYARDQDTAALYRRMGLNTRQIE
ILATAIPKRQYYYVSESGRRLYDLALGPFALAFVGSSDKESVTSIKSLEAKFGDQWVHEWLAGKGLNLND
YLEAA
MIEAIAITIAGVGTVLLLILFARIRQVDAELQLKKHRSKDAGLADLLNYASLVDDGVIVGKNGSFMAAWL
YRGDDNASSTDEQREMVSFRLNQALAGLGSGWMVHVDAVRRPAPNYSAKGSSHFRDAVSAAIDNERRRLF
EGLGTMYEGYFVLTLTWFPPLLAQRKFVELMFDDDAIAPDRQTRTMGLIEQFKRELTAIENRLSSAVKLM
RMRGQKVVNEDGSTVTHDDFLSWLQFCLTGRHHPVLLPSNPVYLDAVIGGQELWGGVVPRIGRNFVQVVA
IEGFPLESTPGLLTALGELPCEYRWSSRFIFMDPHEAVKHLDKFRKKWRQKVRGFFDQVFNTHTGPVDQD
ALSMVGDAEAAIAEVNSDLVAVGYFTGVVILMDENRDKLEESARLVEKSINRLGFAARIETINTLDAFLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGHADAPCPLYPPLSPALMHCVTHGATPFRLNLHVRDLGH
TFMFGPTGAGKSTHLALIAAQLRRYPGMTLYAFDKGMSLYPLTKAVGGLHFSVAADDDRLAFCPLQFLDS
KGDRAWAMEWIDTILALNGVETTPAQRNEIGHAIMSMHRSGARTLSELSVTIQDEAIREAIRQYTVDGTM
GHLLDAEEDGLSLADFTTFEIEELMDLGEKYALPVLLYLFRRIERSLKGQPAVIILDEAWLMLGHPAFRA
KIREWLKVLRKANCLVLMATQSLSDVANSGILDVIVESTATKIFLPNVYARDQDTAALYRRMGLNTRQIE
ILATAIPKRQYYYVSESGRRLYDLALGPFALAFVGSSDKESVTSIKSLEAKFGDQWVHEWLAGKGLNLND
YLEAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 91859..100466
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GO608_RS00435 (GO608_000435) | 87147..88106 | - | 960 | WP_169200551 | site-specific integrase | - |
| GO608_RS00440 (GO608_000440) | 88375..88638 | - | 264 | Protein_88 | transposase | - |
| GO608_RS00445 (GO608_000445) | 88754..89353 | - | 600 | WP_247002723 | muramidase | - |
| GO608_RS00450 (GO608_000450) | 89359..89934 | - | 576 | WP_169200549 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| GO608_RS00455 (GO608_000455) | 89952..91603 | - | 1652 | Protein_91 | P-type conjugative transfer protein TrbL | - |
| GO608_RS00460 (GO608_000460) | 91614..91844 | - | 231 | WP_169199866 | entry exclusion lipoprotein TrbK | - |
| GO608_RS00465 (GO608_000465) | 91859..92617 | - | 759 | WP_169199867 | P-type conjugative transfer protein TrbJ | virB5 |
| GO608_RS00470 (GO608_000470) | 92636..94024 | - | 1389 | WP_169199868 | TrbI/VirB10 family protein | virB10 |
| GO608_RS00475 (GO608_000475) | 94030..94521 | - | 492 | WP_169199910 | conjugal transfer protein TrbH | - |
| GO608_RS00480 (GO608_000480) | 94524..95420 | - | 897 | WP_169199869 | P-type conjugative transfer protein TrbG | virB9 |
| GO608_RS00485 (GO608_000485) | 95438..96223 | - | 786 | WP_169199870 | conjugal transfer protein TrbF | virB8 |
| GO608_RS00490 (GO608_000490) | 96220..98757 | - | 2538 | WP_169199871 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| GO608_RS00495 (GO608_000495) | 98754..99065 | - | 312 | WP_169199872 | conjugal transfer protein TrbD | virB3 |
| GO608_RS00500 (GO608_000500) | 99068..99493 | - | 426 | WP_169199873 | conjugal transfer system pilin TrbC | virB2 |
| GO608_RS00505 (GO608_000505) | 99507..100466 | - | 960 | WP_169199874 | P-type conjugative transfer ATPase TrbB | virB11 |
| GO608_RS00510 (GO608_000510) | 100762..101124 | - | 363 | WP_169199875 | transcriptional regulator | - |
| GO608_RS00515 (GO608_000515) | 101461..102645 | - | 1185 | WP_169199876 | alpha-hydroxy acid oxidase | - |
| GO608_RS00520 (GO608_000520) | 102697..103998 | - | 1302 | WP_169199877 | TRAP transporter large permease | - |
| GO608_RS00525 (GO608_000525) | 103988..104470 | - | 483 | WP_169199878 | TRAP transporter small permease | - |
Host bacterium
| ID | 3036 | GenBank | NZ_WTVH02000001 |
| Plasmid name | pU120 | Incompatibility group | - |
| Plasmid size | 334342 bp | Coordinate of oriT [Strand] | 38975..39073 [-] |
| Host baterium | Aromatoleum buckelii strain U120 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | acn |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA7 |