Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102590
Name   oriT_pLuFRes1 in_silico
Organism   Aromatoleum anaerobium strain LuFRes1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_WTVG02000001 (11151..11248 [+], 98 nt)
oriT length   98 nt
IRs (inverted repeats)      24..30, 36..42  (TAACCGG..CCGGTTA)
Location of nic site      61..62
Conserved sequence flanking the
  nic site    		  TCCTGCCCGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 98 nt

>oriT_pLuFRes1
GAATAAGGGCTAGCGAAGATAGATAACCGGCTCGCCCGGTTAGCTAACTTCGCCCATCCTGCCCGCCTTTCGGCGCTGATTACACCAATGGAAGTCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1937 GenBank   WP_169119845
Name   Relaxase_GO606_RS00035_pLuFRes1 insolico UniProt ID   _
Length   740 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 740 a.a.        Molecular weight: 81617.45 Da        Isoelectric Point: 10.5659

>WP_169119845.1 TraI/MobA(P) family conjugative relaxase [Aromatoleum anaerobium]
MIAKHVPMRSLAKSDFAELVEYITDEQDKAERLGQIAATNCEAATMQAVIGEVLATQRQNTRAAGDKTFH
LLVSFRPGERPTPETLKAIEERICAGLGYGEHQRVSAVHHDTDNLHLHIAINKIHPERHTMHEPFQSYRT
LAELCATLERDYGLEKDNHQSRRSVAEGRAADMECHAGVESLVGWVRRECLEEMRGAQSWTELHQVMKEN
GLELRERGNGLVVEAADGTRVKASTIARELSKPGLEARLGPFQVPPERQAPPKIRRQYDKAPIRLRVNTV
ELYARYKAEQQNLTAARADALTKARRRKDRAVEDAKRANRLRRATIKVVDSKGISKKVLYSQASTALRDR
LAEIHKEYAKEREKLYQGFQRRAWADWLKQEALQGDAEALTALRAREAAQGLKGNTVAGRGEAKPGHAHV
QDNITKKGTIIFRAGQSAVRDDGDKLQVSREATREGVQAALRLAMERYGNQITVNGTPEFKARAIRAAVD
SQLPITFADPALELRRQQLLNKEQAHERAARAERGRTDRRSLGSAGSSAAADDNLARTGAEIGRGRSDGR
GGAGMDAPAHAPRKPNVGRIGRVPPPQSQHRLRGMSELGVVRIASGSEVLLPRDVPRHVEQQGAQPDHAL
RRGVFGSGRLKPAQLAAADKYIAEREGKRLKGVDIPKHTLYNEGASVISFAGVRNVEGQPLALLKRGDAD
AVMVLPIDQATARRLSRVAVGDPITVTPRGSIQTSKGRSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(13-251)

Large polyvalent protein-associated domain 7 (LPD7)

(420-509)


  Protein structure



No available structure.




T4CP

ID   1566 GenBank   WP_169119846
Name   t4cp2_GO606_RS00030_pLuFRes1 insolico UniProt ID   _
Length   634 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 634 a.a.        Molecular weight: 69630.74 Da        Isoelectric Point: 9.0195

>WP_169119846.1 type IV secretory system conjugative DNA transfer family protein [Aromatoleum anaerobium]
MKLKMNNAVGPQVRTKKATGGRLLPALGALSLGGGLQSATQFFAHTFNYQANLGSHFNHVYAPWSILEWS
SKWYSHYPHEIMKAASVGMMVSTVGLLGVAVAKVVSSNSSKANEYLHGSARWAGKKDIQAAGLLPRPRSI
LEVVTGKGASMSTGVYVGGWEDKDGNFHYLRHNGPEHVLTYAPTRSGKGVGLVVPTMLSWGASAVVTDLK
GELWAMTAGWRKKHAKNKVLRFEPATASGSVCWNPLDEIRLGTEYEVGDVQNLATLIVDPNGKGLDSHWQ
KTAFALLVGVILHALYKAKDDGTTATLPLVDAMLADPNRDIGELWMEMATYGHVAGQNHHAIGSTARDMM
DRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSEFRIRDLMHSDDPVTLYIVTQPNDKDRLRPLVRVMVN
MVVRLLADKMDFENGRPVAHYKHRLLMMLDEFPSLGKLQILQESLAFVAGYGIKCYLICQDINQLKSRET
GYGPDETITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTFQEVQRPLLT
PDECLRMPGPRKNADGNIEEAGDMVVYVAGHPAIYGKQPLYFKDPVFQARASVPAPKVSDRLIQFAEAGE
GITI

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(121-621)

  Protein structure



No available structure.



ID   1567 GenBank   WP_169119623
Name   t4cp2_GO606_RS00390_pLuFRes1 insolico UniProt ID   _
Length   850 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 850 a.a.        Molecular weight: 94358.18 Da        Isoelectric Point: 5.9244

>WP_169119623.1 VirB4 family type IV secretion/conjugal transfer ATPase [Aromatoleum anaerobium]
MIQAIAITIAVVGAALLLILFARIRTVDAELQLKKHRSKDAGLADLLNYAAVVDDGVIVGKNGSFMAAWL
YRGDDNASSTDEQREMVSFRINQALAGLGSGWMVHVDAVRRPAPNYSIKGVSHFPDAVSAAIDEERRRLF
EGQGTMYEGYFVLTLTWFPPVLAQRKFVELMFDDDAIAPDHKARTMGLIAQFKREITVIENRLSSAVNLT
RMRGQKVVNEDGSTVTHDDFLSWLQFCITGRHHPVLLPSNPMYLDAVIGGQELWGGVVPKIGRNFVQVVA
IEGFPLESTPGLLTALGELPCEYRWSSRFIFMDQHEAVKHLDKFRKKWRQKVRGFFDQVFNTNTGAVDQD
ALSMVGDAEAAIAEVNSGLVAQGYYTGVVVLMNEDRDKLEESARLVEKSINRLGFAARIETINTQDAFLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGNADAPCPLYPPLSPALMHCVTHGATPFRLNLHVRDLGH
TFMFGPTGAGKSTHLALIAAQLRRYAGMTIYAFDKGMSMYPLTKAVGGRHFSVAADDDRLAFCPLQFLET
KGDRAWAMEWIDTILALNGVETTPAQRNEIGHAIMSMQRSGARTLSEFSVTIQDEAIREAIRQYTVDGTM
GHLLDAEEDGLSLADFTTFEIEELMDLGEKYALPVLLYLFRRIERSLKGQPAVIILDEAWLMLGHPAFRA
KIREWLKVLRKANCLVLMATQSLSDAANSGILDVIVESTATKIFLPNVYARDEDTAALYRRMGLNARQIE
ILATAIPKRQYYYVSENGRRLYDLALGPLALAFVGSSDKDSVATIKSLEAKFGDEWVHEWLAGKGLNLND
YLVDDYLEAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(619-746)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(221-431)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 63755..74242

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO606_RS00340 (GO606_000340) 58825..59190 - 366 WP_211166739 hypothetical protein -
GO606_RS00345 (GO606_000345) 59224..59601 - 378 WP_211166740 hypothetical protein -
GO606_RS00350 (GO606_000350) 59664..60146 - 483 WP_169200683 hypothetical protein -
GO606_RS00355 (GO606_000355) 60188..60622 - 435 WP_169119617 antirestriction protein -
GO606_RS00360 (GO606_000360) 60757..60912 - 156 WP_211166741 hypothetical protein -
GO606_RS00365 (GO606_000365) 61943..62785 - 843 WP_246979202 plasmid replication initiator TrfA -
GO606_RS00370 (GO606_000370) 63101..63463 + 363 WP_169119619 transcriptional regulator -
GO606_RS00375 (GO606_000375) 63755..64714 + 960 WP_169119620 P-type conjugative transfer ATPase TrbB virB11
GO606_RS00380 (GO606_000380) 64728..65153 + 426 WP_169119621 conjugal transfer system pilin TrbC virB2
GO606_RS00385 (GO606_000385) 65156..65467 + 312 WP_169119622 conjugal transfer protein TrbD virB3
GO606_RS00390 (GO606_000390) 65464..68016 + 2553 WP_169119623 VirB4 family type IV secretion/conjugal transfer ATPase virb4
GO606_RS00395 (GO606_000395) 68013..68798 + 786 WP_169119624 conjugal transfer protein TrbF virB8
GO606_RS00400 (GO606_000400) 68816..69712 + 897 WP_169119625 P-type conjugative transfer protein TrbG virB9
GO606_RS00405 (GO606_000405) 69715..70206 + 492 WP_169119626 conjugal transfer protein TrbH -
GO606_RS00410 (GO606_000410) 70212..71600 + 1389 WP_169119627 TrbI/VirB10 family protein virB10
GO606_RS00415 (GO606_000415) 71619..72377 + 759 WP_169119628 P-type conjugative transfer protein TrbJ virB5
GO606_RS00420 (GO606_000420) 72387..72608 + 222 WP_169119629 entry exclusion lipoprotein TrbK -
GO606_RS00425 (GO606_000425) 72620..74242 + 1623 WP_211166742 P-type conjugative transfer protein TrbL virB6
GO606_RS00430 (GO606_000430) 74260..74838 + 579 WP_169119630 TrbM/KikA/MpfK family conjugal transfer protein -
GO606_RS00435 (GO606_000435) 74772..75992 - 1221 WP_246979205 IS110 family transposase -
GO606_RS00440 (GO606_000440) 76258..76389 + 132 WP_246979231 TrbM/KikA/MpfK family conjugal transfer protein -
GO606_RS00445 (GO606_000445) 76395..76991 + 597 WP_169120740 muramidase -
GO606_RS00450 (GO606_000450) 76988..77686 + 699 WP_169120738 TraX family protein -
GO606_RS00455 (GO606_000455) 77922..78122 + 201 Protein_90 IS3 family transposase -
GO606_RS00460 (GO606_000460) 78119..78775 + 657 WP_169120736 hypothetical protein -
GO606_RS00465 (GO606_000465) 78768..79040 + 273 WP_169120734 ribbon-helix-helix domain-containing protein -

Host bacterium

ID   3034 GenBank   NZ_WTVG02000001
Plasmid name   pLuFRes1 Incompatibility group   -
Plasmid size   115562 bp Coordinate of oriT [Strand]   11151..11248 [+]
Host baterium   Aromatoleum anaerobium strain LuFRes1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   acn
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIF24