Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102586
• Name: oriT_pA12b
• Organism: Rhizobium tropici strain A12
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JAADZA010000042 (7581..7616 [-], 36 nt)
• oriT length: 36 nt
• IRs (inverted repeats): 21..26, 31..36 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 36 nt

>oriT_pA12b
ATCCGAAGGGCGCAATTATACGTCGCTGGCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_GXW80_RS25635_pA12b

• ID: 1934
• GenBank: WP_004119830
• Name: traA_GXW80_RS25635_pA12b
• UniProt ID: A0A6P1CE10
• Length: 1103 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1103 a.a.
• Molecular weight: 122039.84 Da
• Isoelectric Point: 10.0272

>WP_004119830.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Rhizobium]
MAVPHFSVSIVARGSGRSAVLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVIPADAPSWLRTMIADR
SVSGASEAFWNSVEIFEKRVDAQLAKDVTVALPMELSSEQNIELVRDFIERHITSKGMIADWVYHDAPGN
PHVHLMTTLRPLTEDGFGAKKVAVTGPGGKPVRNDAGKIVYELWAGSLDDFNAFRDGWFACQNRHLARAG
LDLRVDGHSFEKQGINLTPTIHLGVGAKAIERKSAEAERAPSLERLELQEERRAENARRIQRRPELVLDL
ITREKSVFDERDVAKILHRYIDDAGLFQSLMARILQSPETLRLERERIEFSGGLRVPTKYTTRDLVRLEA
EMANRAIWLAQRSSHGVADAVREATFARHLRLADEQKTAIAHVTGGERIAAVIGRAGAGKTTMMKAAREA
WEAAGYRVVGGALAGKAAEGLEKEAGILSRTLSSWELRWNQGRNQLDDKTVFVLDEAGMVSSRQMALFVE
AATKAGAKLVLVGDPEQLQPIEAGAAFRAIADRIGYGELENIYRQREQWMREASLDLARGNIGRAVEAYR
AQGRVSGADLKADAVQALIADWNRDYDPQRSSLILAHLRRDVRMLNGMARAKLVERGLVADGFAFRTEDG
HRKFAAGDQIVFLKNEGSLGVKNGMLAKVVDATPGRIVAEVGDGEHRRQVTVEQRFYANLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDFGLYHGRRSFAKAGGLIPILSRRQAKETTLDYASGRFY
AQALRFADARGLHLVNVARTVVRDRIEWTVRQKSKLAALGARLAAIARRLGLSAGAAKTSQSHSMQEAKP
MVSGITTFPKSLEQVVEDLLAADAGLKKQWEEVSARFHLVYAQPEDAFKAVNVDAMLRNEAAAKSTITKI
GSEPETFGALKGKTGILAGRADKQAHERAVTNAPALARNLERYLRQRTEAEHKYDAEERAVRMKISLDIP
ALSPAAKQTLERVRDAIDRNDLPAGLENALADKMVKAELEGFARAVSERFGERTFLPLAAKDMTGQTFQS
VTSSMSAGQKAEVQSAWSTMRTVQQLSAHERTTEALKQAETLRQTKSQGLSLK

Protein domains

Predicted by InterproScan.

• Viral (Superfamily 1) RNA helicase (Viral_helicase1) (692-735)

• MobA/MobL family (MobA_MobL) (17-243)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (860-955)

• AAA domain (AAA_30) (382-568)

Protein structure

• AlphaFold DB: A0A6P1CE10

T4CP

traG_GXW80_RS25650_pA12b

• ID: 1564
• GenBank: WP_004119814
• Name: traG_GXW80_RS25650_pA12b
• UniProt ID: _
• Length: 658 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 658 a.a.
• Molecular weight: 71115.08 Da
• Isoelectric Point: 9.0145

>WP_004119814.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [Rhizobium]
MTRILLFVAPCALMMLMAIGMTGTELWLSHFGKSDAARQMLGRAGIALPYVAASVVGIIFLFASAGSARI
RTAGWGVVTGATATLVLAVLREASRLSAYLEQVPAGKTVFNYLDPATAIGAAAVLMSALFGMRVAIAGNA
TFARAEPKRIVGKRALHGEADWMKLSQAEKLFAESGGIVIGERYRVDRDSIAAPSFRADSAASWGAGGKS
PLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGALVVLDPSNEVAPMVLKHRGDANRDVFVLDPK
HSEIGFNALDWIGRFGGTKEEDIASVASWIMSDSGGARGVRDDFFRASALQLLTALIADVCLSGHTTEND
QTLRQVRKNLSEPEPQLRQRLHEIYDDSNSDFVKENVAAFVNMTPETFSGVYANAVKETHWLSYPNYAAL
VSGTSFSTSDLADGKTDVFINIDLKTLETHAGLARVIIGAFLNAIYNRNGEMQGRALFLLDEVARLGYMR
ILETARDAGRKYGITLTMIYQSIGQMRETYGGRDAASKWFESASWISFAAINDPETADYISRRCGMTTVE
IDQVSRSVQSKGSSRTRSKQLAARPLIQPHEVLRMRADEQIVFTAGNAPLRCGRAIWFRRDDMKGCVGAR
AFSKAANPAENSPDRPARSAASKADREQ

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (161-633)

Protein structure

No available structure.

Host bacterium

• ID: 3030
• GenBank: NZ_JAADZA010000042
• Plasmid name: pA12b
• Incompatibility group: -
• Plasmid size: 45675 bp
• Coordinate of oriT [Strand]: 7581..7616 [-]
• Host baterium: Rhizobium tropici strain A12

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: nodM
• Anti-CRISPR: -