Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102584
• Name: oriT_pSM135A_Rh02
• Organism: Rhizobium leguminosarum strain SM135A
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_SIOO01000003 (81373..81401 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSM135A_Rh02
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_ELH89_RS30065_pSM135A_Rh02

• ID: 1932
• GenBank: WP_130751505
• Name: traA_ELH89_RS30065_pSM135A_Rh02
• UniProt ID: _
• Length: 1536 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1536 a.a.
• Molecular weight: 169711.00 Da
• Isoelectric Point: 8.6768

>WP_130751505.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVIGRGAGRSVVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELALPDQIPAWLRSAISG
QSVAKASETLWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVLDNLTAKGMVADWVYHDKDG
NPHIHLMTTLRPLAEEGFGPKKVPVRGEDGKPLRVVTPDRPNGKVVYKLWAGDKETLQGWKIAWAETASR
HLALAGHEIRLDGRSYAEQGLDGIAQRHLGPEKAALSRRDAEMYFAPADLARRQKMADRLLADPALLLKQ
LGNERSTFDERDIAKALHRTVDDPADFANIRARLMASDDLIMLKPQQIDAETGKAREPAVFTTREMLRIE
YDMGRSARVLAERRGFAVSDRVVAAAIRQVETQDPENSFRLDPEQVDAIHHITRDNAIAAVVGLAGAGKS
TLLAAARVAWESDGRRVIGAALAGKAAEGLEDSSGIRSRTLAAWELAWASGRELLERDNVLVIDEAGMVS
SQQMARMLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQPWAREASRLFARGE
TGKGLDAYAQQGHLIEAGTRVETIDRIVADWSQARKQAIQNSTTAGNDGRLRGDELLVLAHTNEDVKYLN
EALRSVMVGEGALGEDRAFRTERGARAFAAGDRIIFLENARFVEPRATRLGPQHVKNGMLGTVVSTGDKR
GDAVLSVRLDSGRDVVISESSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRVDL
YAAKDDFEAKPEWGRKPHVDHAAGVTGALVETGQAKFRPNDEDADDSPYADVRADDGTLHRLWGVSLPKA
LEEGGVSEGDSVTLRKDGVERVTIKFAIVDEQTGQKRFEEREVDRNVWTARRIETAEARQQRIERESHRP
DVFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDHLSLAAAGVEESLSRRWAGIAEKREQLA
KLWERASVALGFTIEREPRIAYNEERAEPQTVLEADVARGRYLIPPTTGFARSAEEDARLAQLSSPAWKE
REAILRPLLETIYRDPDAALVALNALSSDTSVDPRRLADELAKAPERLGRLRGSDLIVDGRAARDERNAA
ITALTDLLPLVRAHATQFRRNAERFESHEQQRRAHMALPIPALSEKVMARLTEIEAVRRQGGDDAYKTAF
ALAAKDRTIVQEVKAVSEALTARFGWSAFTAKADAVGERNMVERMPEDLASIRGEKLTRLFKAVKRFADE
QHLAERRDRSKIVAGASADQGMENVPMLAAVTEFRTAIDEEARSRVLSDPLYRQQRAALAAVASAIWRDP
VGAVGKIEELLQKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLAAGRERKEAVQAVPEAAARLRSLGP
AYVNVLDAERQAIAEERRRMAVAIPALSTAAEDVLMRLTAEAKNNDRKLTASAAPLDPSIRREFAAVSRA
LDERFGRNAIIRGEKDLINRVPPAQRNAFEAMQDRLKVLQRTVRMESSEQIISERRQRAVSRGISL

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1127)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1305-1399)

• AAA domain (AAA_30) (391-576)

• MobA/MobL family (MobA_MobL) (17-249)

Protein structure

No available structure.

Host bacterium

• ID: 3028
• GenBank: NZ_SIOO01000003
• Plasmid name: pSM135A_Rh02
• Incompatibility group: -
• Plasmid size: 618182 bp
• Coordinate of oriT [Strand]: 81373..81401 [+]
• Host baterium: Rhizobium leguminosarum strain SM135A

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -