Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102580
• Name: oriT_pSM79_Rh02
• Organism: Rhizobium leguminosarum strain SM79
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_SIME01000003 (436917..436945 [-], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSM79_Rh02
AGGGCGCAATATACGTCGCTATCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_ELH27_RS29480_pSM79_Rh02

• ID: 1928
• GenBank: WP_130767144
• Name: traA_ELH27_RS29480_pSM79_Rh02
• UniProt ID: _
• Length: 1538 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1538 a.a.
• Molecular weight: 170309.51 Da
• Isoelectric Point: 8.7850

>WP_130767144.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVISRGAGRSIVSAAAYRHRTRMMDEQAGTSFSYRGGASELVHEELALPDQMPTWLRTAIDG
RSVAGASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTTLRSLTEEGFGPKKVAVAGEDGKPLRVVTPDRPKGRIVYKLWAGDKETMKAWKVAWAETANR
HLALAGHEIRLDGRPYADQGLDGIAQRHLGPEKAALSRRGAEMYFAPADLARRQEMADRLLADPALLLKQ
LSNERSTFDERDIAKALHRTVDDPAVFANIRARLMASDDLIMLKPQQLDAETGKASEPAVFTTREVLRIE
YDMARSAQLLAECRGFGVSDRVVAAAIRHVETRDTEKSFRLDPEQVDAVRHVTGDNGITAVVGLAGAGKS
TLLAAARVAWESDGRRVIGAALAGKAAEGLEDSSGIRSRTLAAWELAWANGRELPERGNVLVIDEAGMVS
SQQLARVLDIAEQAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRHQWAREASRLFARGE
VEQGLDAYARHGHLIEAGTRDEVIGRIVGDWTDARRQAIRNSASAGNDGRLRGDELLVLAHTNEDVKRLN
EALRSVMSQEGALSENRAFRTERGAREFAVGDRIIFLENARFLEPRAPRLGPQHVKNGMLGTVVSTGDKR
GDTLLSVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGATVDRTLVLATGMMDQHLTYVSMTRHRDRVDL
YAAKEDFEAKPEWGRKPRVDHAAGVTGALVESGQAKFRPNDEDADDSPYADVRTDDGTIHRLWGVSLPKA
LEEGGISQGDTVTLRKDGVERVTIKVSVVDEQTGQKRFEEREVDRNVWTAKRIETAAERQQRIEQESHRP
DLFKQLVERLSRSGAKTTTLDFESEASYRAQAQDFARRRGLDHLSLAAAAVEENLSRRWAGIAERRKQLA
KLWERAGVAFGFTIERERRVAYSEERAEPQTVLEADAARVRYLIPPATGFARSAEEDARLAQLASPAWKE
REALLRPLLETIYRDPDAALVALNALSSDTSIEPRRLADDLANAPDRLGRLRGSDLIIDGRAARDERNTA
ITALRDLLPLARAHATEFRRNGERFDLGEQRRRTHMSLPIPALSEKAMARLMEIEAVRRQGGDDAYKTAF
ALATEDRSIVQEVKAVSEALSARFGWSAFTAKADAVAERNMIERMPEDLTSISGEKLTRLFEAVKRFADE
QHLVERRDRSKIVAAASADPQKENVPMLAAVTEFKTAVDEEARSRALATPLYRQQRAALAAVASTIWRDP
AGAVGKIEELLQKGFAGERIAAAVTNDPSAYGALRGSDRLMVRMLAAGRERKKAVQAMPEAGARLRALGS
AYVNLLDGERQAITEERRRMAVAIPGLSKAAEDVLMRLTAEAKNNGRKLSAPDASLDPTIRREFAAVSTA
LDERFGRNAIIRGEKDLVNRVPHAQHHAFEVMQEKLKVLQQTVRRESNEQIISERRQRAVSRGRGMDL

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1308-1398)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1045-1126)

• MobA/MobL family (MobA_MobL) (17-249)

• AAA domain (AAA_30) (391-576)

Protein structure

No available structure.

Host bacterium

• ID: 3024
• GenBank: NZ_SIME01000003
• Plasmid name: pSM79_Rh02
• Incompatibility group: -
• Plasmid size: 475349 bp
• Coordinate of oriT [Strand]: 436917..436945 [-]
• Host baterium: Rhizobium leguminosarum strain SM79

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIF11