Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102572
Name   oriT_FWSEC0118|unnamed1 in_silico
Organism   Escherichia coli strain FWSEC0118
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRGJ01000112 (24526..24879 [-], 354 nt)
oriT length   354 nt
IRs (inverted repeats)      192..198, 206..212  (TATAAAA..TTTTATA)
 197..202, 204..209  (AAAATA..TATTTT)
 186..192, 196..202  (TATTTTT..AAAAATA)
 104..110, 118..124  (TTTAAAT..ATTTAAA)
 106..111, 113..118  (TAAATC..GATTTA)
 93..98, 106..111  (GATTTA..TAAATC)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 354 nt

>oriT_FWSEC0118|unnamed1
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCGTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATCTGATTTATTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTAACTATTTTTATAAAAAATATTATTTTATATTAGGGGGGGGCTGCTAGCGGCGCGGTGTATTTTTTTATAGGATACCGCCAGGGGCGCTGCTAGCGGTGCGTCCCTGCTTGCATTATGAACTCTAGTGTTTCGAAATTAACTTTATTTTATGTTCAAAAAAGGTAATCTCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   1544 GenBank   WP_136801699
Name   traC_C9Z68_RS25075_FWSEC0118|unnamed1 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99326.11 Da        Isoelectric Point: 6.0813

>WP_136801699.1 type IV secretion system protein TraC [Escherichia coli]
MNNPLEAVTQAVNSLLTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRKSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRCVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNFKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraC protein (TraC_F_IV)

(38-276)

TraG P-loop domain (P-loop_TraG)

(467-771)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1604..25450

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9Z68_RS24975 (C9Z68_25025) 328..1059 - 732 WP_040090739 conjugal transfer complement resistance protein TraT -
C9Z68_RS24980 (C9Z68_25030) 1091..1588 - 498 WP_040090738 entry exclusion protein -
C9Z68_RS24985 (C9Z68_25035) 1604..4426 - 2823 WP_047088737 conjugal transfer mating-pair stabilization protein TraG traG
C9Z68_RS24990 (C9Z68_25040) 4423..5796 - 1374 WP_024212142 conjugal transfer pilus assembly protein TraH traH
C9Z68_RS24995 (C9Z68_25045) 5783..6175 - 393 WP_040091326 F-type conjugal transfer protein TrbF -
C9Z68_RS25000 (C9Z68_25050) 6129..6443 - 315 WP_064236902 P-type conjugative transfer protein TrbJ -
C9Z68_RS25005 (C9Z68_25055) 6433..6969 - 537 WP_040091329 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
C9Z68_RS25010 (C9Z68_25060) 6956..7237 - 282 WP_000049684 type-F conjugative transfer system pilin chaperone TraQ -
C9Z68_RS27825 (C9Z68_25070) 7616..7979 - 364 Protein_8 hypothetical protein -
C9Z68_RS25025 (C9Z68_25075) 7993..8736 - 744 WP_040091331 type-F conjugative transfer system pilin assembly protein TraF traF
C9Z68_RS25030 (C9Z68_25080) 8729..8944 - 216 WP_244591685 conjugal transfer protein TrbE -
C9Z68_RS25035 (C9Z68_25085) 9000..10904 - 1905 WP_236927347 type-F conjugative transfer system mating-pair stabilization protein TraN traN
C9Z68_RS25040 (C9Z68_25090) 10929..11150 + 222 WP_135276000 hypothetical protein -
C9Z68_RS25045 (C9Z68_25095) 11139..11519 - 381 WP_024213661 hypothetical protein -
C9Z68_RS25050 (C9Z68_25100) 11516..12154 - 639 WP_040091622 type-F conjugative transfer system pilin assembly protein TrbC trbC
C9Z68_RS25055 (C9Z68_25105) 12181..12699 - 519 WP_064236900 hypothetical protein -
C9Z68_RS25060 (C9Z68_25110) 12975..13967 - 993 WP_047088909 conjugal transfer pilus assembly protein TraU traU
C9Z68_RS25065 (C9Z68_25115) 13964..14596 - 633 WP_064236899 type-F conjugative transfer system protein TraW traW
C9Z68_RS25070 (C9Z68_25120) 14593..14979 - 387 WP_047088907 type-F conjugative transfer system protein TrbI -
C9Z68_RS25075 (C9Z68_25125) 14976..17603 - 2628 WP_136801699 type IV secretion system protein TraC virb4
C9Z68_RS25080 (C9Z68_25130) 17763..17984 - 222 WP_042966711 conjugal transfer protein TraR -
C9Z68_RS25085 (C9Z68_25135) 18119..18634 - 516 WP_047088905 type IV conjugative transfer system lipoprotein TraV traV
C9Z68_RS25090 (C9Z68_25140) 18631..18951 - 321 WP_047088904 conjugal transfer protein TrbD -
C9Z68_RS25095 (C9Z68_25145) 18938..19525 - 588 WP_047088903 conjugal transfer pilus-stabilizing protein TraP -
C9Z68_RS25100 (C9Z68_25150) 19515..20933 - 1419 WP_047088902 F-type conjugal transfer pilus assembly protein TraB traB
C9Z68_RS25105 (C9Z68_25155) 20933..21661 - 729 WP_001355329 type-F conjugative transfer system secretin TraK traK
C9Z68_RS25110 (C9Z68_25160) 21648..22214 - 567 WP_000399757 type IV conjugative transfer system protein TraE traE
C9Z68_RS25115 (C9Z68_25165) 22236..22547 - 312 WP_000012108 type IV conjugative transfer system protein TraL traL
C9Z68_RS25120 (C9Z68_25170) 22562..22921 - 360 WP_000340268 type IV conjugative transfer system pilin TraA -
C9Z68_RS25125 (C9Z68_25175) 22955..23182 - 228 WP_000589558 conjugal transfer relaxosome protein TraY -
C9Z68_RS25130 (C9Z68_25180) 23301..23948 - 648 WP_042966703 transcriptional regulator TraJ family protein -
C9Z68_RS25135 (C9Z68_25185) 24148..24525 - 378 WP_001151539 conjugal transfer relaxosome DNA-binding protein TraM -
C9Z68_RS25140 (C9Z68_25190) 24803..25450 + 648 WP_135276001 transglycosylase SLT domain-containing protein virB1
C9Z68_RS25145 (C9Z68_25195) 25747..26568 - 822 Protein_33 DUF932 domain-containing protein -
C9Z68_RS25150 (C9Z68_25200) 26687..26974 - 288 WP_000107542 hypothetical protein -
C9Z68_RS25155 (C9Z68_25205) 27225..28229 + 1005 WP_024166184 IS110 family transposase -

Host bacterium

ID   3016 GenBank   NZ_RRGJ01000112
Plasmid name   FWSEC0118|unnamed1 Incompatibility group   -
Plasmid size   28471 bp Coordinate of oriT [Strand]   24526..24879 [-]
Host baterium   Escherichia coli strain FWSEC0118

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -