Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102567
• Name: oriT_pSM45_Rh02
• Organism: Rhizobium leguminosarum strain SM45
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_SILA01000003 (4299..4327 [-], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSM45_Rh02
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_ELG97_RS30025_pSM45_Rh02

• ID: 1917
• GenBank: WP_130769396
• Name: traA_ELG97_RS30025_pSM45_Rh02
• UniProt ID: _
• Length: 1425 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1425 a.a.
• Molecular weight: 157384.42 Da
• Isoelectric Point: 6.7478

>WP_130769396.1 Ti-type conjugative transfer relaxase TraA, partial [Rhizobium leguminosarum]
MAIMFVRAQVIGRGAGRSVVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELALPDQTPAWLRSAVEG
RSVAGASEALWNAVEAFEKQANARLARELIIALPEELTRAENIALVREFVRDNFTSERMVADWVYHDKDG
NPHIHLLTTVRPLTEEGFGPKNAPVLGENGEPLRFVTARRPTGRIVYKSWGGDKETLQAWKIAWAETASR
HLALAGHEIRLDGRSYAEQGLDGIAQRHLGPEKAALARRDAEMYFAPADLARRQDMADRLLADPALLLKQ
LSNERSTFDERDIAKALHRTVDDPADFANIRARLMASDDLIMLKPQQIDAETGKASEPAVFTTREMLRIE
YDMGRSARVLAERRGFAVSDRVVAAAIRQVETQDPENSFRLDPEQVDAIHHITRDNAIAAVVGLAGAGKS
TLLAAARVAWESDGRRVIGAALASKAAEGLEDSSGIRSRTLAAWELAWASGRELLERGNVLVIDEAGMVS
SQQMARLLDIAEQAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQPWAREASRLFARGE
TGKGLDAYAQQGHLIEAGTRAETIDRIVADWTEARKQAIETSVLDGNDGRLRGDELLVLAHTNEDVKYLN
EALRSVMVGEGALGEDRAFRTERGARAFAAGDRIIFLENARFVEPRAMRLGPQHVKNGMLGTVVSTGDKR
GDAVLSVRLDSGRDVVISESSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRVDL
YAAKDDFEAKPEWGRKPHVDHAAGVTGALVETGQAKFRPNDEDADDSPYADVRADDGTLHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVDRVTIKVAIADEQTGQKRFKEREVDRNVWTARRIETAAERQQRIERESHRP
DVFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDHLSLAAAGAEESLSRRWAGIAEKREQLA
KLWERASVALGFTIERERRIAYNEERAEPQTVLEPDAARGRYLIPPTTGFARSAEEDARLAQLSSPAWKE
REAILRPLLETIYRDPDAALVALNALSSDTSVEPRRLADDLAKAPERLGRLRGSELIVDGRAARDERNAA
ITALTDLLPLVRAHATRFRRNAERFESHEQQRRAHMALPIPALSEKAMARLTEIEAVRRQGGDDAYKTAF
ALATEDRTIVQEVKAVSEALTARFGWSAFTSKADAVGERNMIERMPEDLASIRGEKLTRLFKAVKRFADE
QYLAERRDRSKIVAGASADQGTENVPMLAAVTEFRTAIDEEARSRVLSDPLYRQQRAALAAVASAIWRDP
VGAVGKIEELLQKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLAAGRERKQAVQAVPEAAARLRSLGS
AYVNVLDAERQAIAEERRRMAVAIP

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1305-1399)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1127)

• AAA domain (AAA_30) (391-576)

Protein structure

No available structure.

Host bacterium

• ID: 3011
• GenBank: NZ_SILA01000003
• Plasmid name: pSM45_Rh02
• Incompatibility group: -
• Plasmid size: 435816 bp
• Coordinate of oriT [Strand]: 4299..4327 [-]
• Host baterium: Rhizobium leguminosarum strain SM45

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -