Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102530
• Name: oriT_pSM141B_Rh02
• Organism: Rhizobium leguminosarum strain SM141B
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_SIOY01000003 (4041..4069 [-], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSM141B_Rh02
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_ELH99_RS30525_pSM141B_Rh02

• ID: 1881
• GenBank: WP_130748239
• Name: traA_ELH99_RS30525_pSM141B_Rh02
• UniProt ID: _
• Length: 1338 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1338 a.a.
• Molecular weight: 147825.73 Da
• Isoelectric Point: 6.5891

>WP_130748239.1 Ti-type conjugative transfer relaxase TraA, partial [Rhizobium leguminosarum]
MAIMFVRAQVIGRGAGRSVVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELALPDQIPAWLRSAISG
QSVAKASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVLDNLTAKGMVADWVYHDKDG
NPHIHLMTTLRPLAEEGFGPKKVPVCGEDGKPLRVVTPDRPNGKVVYKLWAGDKETLQAWKIAWAETASR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRDAEIYFAPADLARRQEMADRLLADPALLLKQ
LSNERSTFDERDIAKALHRTVDDPADFANIRARLMASDDLIMLKPQQIDAETGKASEPAVFTTREMLRIE
YDMGRSARVLAERRGFAVSDRVVAAAIRQVETQDPENSFRLDPEQVDAIHHITRDDGIAAVVGLAGAGKS
TLLAAARVAWESDGRRVIGAALAGKAAEGLEDSSGIRSRTLAAWELAWASGRELLERGNVLVIDEAGMVS
SQQMARLLDIAEQAEVKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQPWAREASRLFARGE
TGKGLDAYAQQGHLIEAGTRAETIDRIVADWSQARKQAIQNSTTAGNDDRLRGDELLVLAHTNEDVKRLN
EALRSVMVGEGALGENRAFRTERGAREFAAGDRIIFLENARFVEPRAMRLGPQHVKNGMLGTVVSTGDKR
GDAVLSVRLDSGRDVVISESSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRVDL
YAAKDDFEAKPEWGRKPHVDHAAGVTGALVETGQAKFRPNDEDADDSPYADVRTDDGTLHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVERVTIKVAIVDEKTGQKRFEEREVDRNVWTARRIETAAERQQRIERESHRP
DVFKQLVERLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDHLSLAAAGVEESLSRRWAGIAEKREQLA
KLWERASVALGFTIERERRIAYNEERAEPQTVLEADAARGRYLIPPTTGFARSAEEDAQLAQLSSPAWKE
REAILRPVLETIYRDPDAALVALNALSSDTSVEPRRLADDLAKAPERLGRLRGSDLIVDGRAARDERNAA
ITALTDLLPLVRAHATQFRRNAERFESHEQQRRAHMALPIPALSEKAMARLTEIEAVRRQGGDDAYKTAF
ALAAKDRSIVQEVKAVSEALTARFGWRAFTSKADAVGERNMIERMPEDLASIRGEKLTRLFDAVKRFADE
QHLAERRDRSKIVAGASADQGMENVPMLAAVTEFRTAIDEEARSRVLSDPLYRQQRAALAAVASAIWRDP
VGAVGKIE

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1049-1127)

• AAA domain (AAA_30) (391-576)

Protein structure

No available structure.

Host bacterium

• ID: 2974
• GenBank: NZ_SIOY01000003
• Plasmid name: pSM141B_Rh02
• Incompatibility group: -
• Plasmid size: 573478 bp
• Coordinate of oriT [Strand]: 4041..4069 [-]
• Host baterium: Rhizobium leguminosarum strain SM141B

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -