Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102526 |
| Name | oriT_pSM154C_Rh03  |
| Organism | Rhizobium leguminosarum strain SM154C |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_SIQC01000004 (174105..174133 [-], 29 nt) |
| oriT length | 29 nt |
| IRs (inverted repeats) | 14..19, 24..29 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 29 nt
>oriT_pSM154C_Rh03
AGGGCGCAATATACGTCGCTGTGGCGACG
AGGGCGCAATATACGTCGCTGTGGCGACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1877 | GenBank | WP_130685247 |
| Name | traA_ELI29_RS30830_pSM154C_Rh03 |
UniProt ID | _ |
| Length | 1545 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1545 a.a. Molecular weight: 170765.73 Da Isoelectric Point: 6.9962
>WP_130685247.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELSLPDQLPAWLRSAIEG
RSVAGASEALWNAVDAFEKRADAQLARELIIAVPEELTRGENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHVMTTLRPMTQEGFGPKKVAVTGEDGNSLRVVTPDRPNGKIVYKLWAGDKETMKVWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQRHLGPEKAALSRRGAEMYFAPADLARRQEMADRLLADPELLLKQ
LGNERSTFDERDIAKALHRYVDDPAVFANIRARLMVSDDLVLLKPQQVDAETAKVAEPAVFTTRELLRIE
YDMARSAQVLSEHKGFAVSDRAVAAAIRHVETGDPEKSFRLDPEQVDAVRHVTRDNGIAAVVGLAGTGKS
TLLAAARVAWESGGSRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWANGHETLHRGDVVVVDEAGMVS
SQQMARVLDIVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQPWAREASRLFARGE
IEKGLDAYAQQGHLIEAGTRAETIDRIVADWSEARKRAIETAALKGNDGRLRGDELLVLAHTNQDVKRLN
EALRTVMTGEGALGENRSFQTERGGRDFAAGDRIIFLENARFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GDTLLSVRLDNGRDVVISENSYRNVDHGYAATIHKSQGSTVERTLVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEARPEWGRKPRVDHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTEDGSVHRLWGVSLPKA
LEEGGVSQGDTVTLRKDGVERVKVQVPIVDEQTGQKRYEEREVDRNVWTAKRIETAAERLERIELESHRP
ELFKQLVERLSRSGAKTTTLDFESEAGYRQYADDFARRRGLDHLSQTAAGMEEGVSRRWAAIAEKREQLA
ELWERASVALGLAIERERRASYNEERIETQTVPDADAGRARYLIPSTTEFARSAEEDARLAQLGSPAWKD
REAILRPLLEKIYRDPDAALVALNALASDNTIEPRRLADDLAVAPDRLGRVRGSDLMVDGRAARDERSIA
IAALTELIPLVRAHATEFRRNAERFGVREQQRRAHMPLPIPALSKAAMARLVEIEAVRRQGGDDAYKTAF
ALATEDRSLVQEVKAVSEALSARFGWSAFTPNADALAERNMVERMPEDLASTRREKLTRLFEAVKHFADE
QHLAERRDRSKIVAGASADQGMEAGKENAAVLPMLAAVTEFKTPIDEEARSRTLATPLYRQQRTALAAVA
STIWRDPAGAVGKIEDLLAKGFAGDRIAAAVTNDPAAYGALRGSDRLMDRMLASGRERKEAVQAVPEAAA
RLRALGSAYVNVLDGERQAVAEERRRMAVAIPGLSKAAEDVLMRLTTGAKNNGRKKNASAASLDPDIRRE
FEAVSRALDERFGRSAILRDEKDIINRIPPAQRRAFEAMQERFKVLQQTVRFESSEQIMSERRQRAVSRG
HGIDL
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELSLPDQLPAWLRSAIEG
RSVAGASEALWNAVDAFEKRADAQLARELIIAVPEELTRGENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHVMTTLRPMTQEGFGPKKVAVTGEDGNSLRVVTPDRPNGKIVYKLWAGDKETMKVWKIAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQRHLGPEKAALSRRGAEMYFAPADLARRQEMADRLLADPELLLKQ
LGNERSTFDERDIAKALHRYVDDPAVFANIRARLMVSDDLVLLKPQQVDAETAKVAEPAVFTTRELLRIE
YDMARSAQVLSEHKGFAVSDRAVAAAIRHVETGDPEKSFRLDPEQVDAVRHVTRDNGIAAVVGLAGTGKS
TLLAAARVAWESGGSRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWANGHETLHRGDVVVVDEAGMVS
SQQMARVLDIVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQPWAREASRLFARGE
IEKGLDAYAQQGHLIEAGTRAETIDRIVADWSEARKRAIETAALKGNDGRLRGDELLVLAHTNQDVKRLN
EALRTVMTGEGALGENRSFQTERGGRDFAAGDRIIFLENARFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GDTLLSVRLDNGRDVVISENSYRNVDHGYAATIHKSQGSTVERTLVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEARPEWGRKPRVDHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTEDGSVHRLWGVSLPKA
LEEGGVSQGDTVTLRKDGVERVKVQVPIVDEQTGQKRYEEREVDRNVWTAKRIETAAERLERIELESHRP
ELFKQLVERLSRSGAKTTTLDFESEAGYRQYADDFARRRGLDHLSQTAAGMEEGVSRRWAAIAEKREQLA
ELWERASVALGLAIERERRASYNEERIETQTVPDADAGRARYLIPSTTEFARSAEEDARLAQLGSPAWKD
REAILRPLLEKIYRDPDAALVALNALASDNTIEPRRLADDLAVAPDRLGRVRGSDLMVDGRAARDERSIA
IAALTELIPLVRAHATEFRRNAERFGVREQQRRAHMPLPIPALSKAAMARLVEIEAVRRQGGDDAYKTAF
ALATEDRSLVQEVKAVSEALSARFGWSAFTPNADALAERNMVERMPEDLASTRREKLTRLFEAVKHFADE
QHLAERRDRSKIVAGASADQGMEAGKENAAVLPMLAAVTEFKTPIDEEARSRTLATPLYRQQRTALAAVA
STIWRDPAGAVGKIEDLLAKGFAGDRIAAAVTNDPAAYGALRGSDRLMDRMLASGRERKEAVQAVPEAAA
RLRALGSAYVNVLDGERQAVAEERRRMAVAIPGLSKAAEDVLMRLTTGAKNNGRKKNASAASLDPDIRRE
FEAVSRALDERFGRSAILRDEKDIINRIPPAQRRAFEAMQERFKVLQQTVRFESSEQIMSERRQRAVSRG
HGIDL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 1476 | GenBank | WP_130671194 |
| Name | traG_ELI29_RS30845_pSM154C_Rh03 |
UniProt ID | _ |
| Length | 637 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 637 a.a. Molecular weight: 70381.05 Da Isoelectric Point: 8.7930
>WP_130671194.1 Ti-type conjugative transfer system protein TraG [Rhizobium leguminosarum]
MALKGRLYPSLLLVFVPIALTAIAVYITGWRWPALAHGLSGKTEYWFLRVAPVPALLFGPLAGLLTVWAL
PLHRRRPVAMASLFCFLLVAGFYTLREYGRLAPSVERGAVTWDHALSYLDFVAVIGALAGFIAVAAAARI
SVVVPEPVKRAKGGAFGDADWLSMSAAARLFPPDGEIVVGERYRVDKEVVHELPFDPNDRSTWGQGGKAP
LLTYSQDFDSTHMLFFAGSGGYKTTSNVLPTALRYTGPLICLDPSTEVAPMVVEHRTRVLGREVMVLDPT
NPITGFNVLDGIEHSQRKEEDIVGIAHMLLSESLRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRN
LRSLRQIVSEPEPSVLAMLRDIQEHSESAFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNAFKSSDIVSDKKDVFLNIPASILRSYPGIGRVIIGSLINAMVQADGGFSRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLLYQSVGQLERHFGKDGATSWIDGCAFASYAAIKALDTARNISAQCGEMTVEVQG
RSRNIGWDTKGGGTRKSESVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKEMDAQAK
ANRFVRT
MALKGRLYPSLLLVFVPIALTAIAVYITGWRWPALAHGLSGKTEYWFLRVAPVPALLFGPLAGLLTVWAL
PLHRRRPVAMASLFCFLLVAGFYTLREYGRLAPSVERGAVTWDHALSYLDFVAVIGALAGFIAVAAAARI
SVVVPEPVKRAKGGAFGDADWLSMSAAARLFPPDGEIVVGERYRVDKEVVHELPFDPNDRSTWGQGGKAP
LLTYSQDFDSTHMLFFAGSGGYKTTSNVLPTALRYTGPLICLDPSTEVAPMVVEHRTRVLGREVMVLDPT
NPITGFNVLDGIEHSQRKEEDIVGIAHMLLSESLRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRN
LRSLRQIVSEPEPSVLAMLRDIQEHSESAFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNAFKSSDIVSDKKDVFLNIPASILRSYPGIGRVIIGSLINAMVQADGGFSRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLLYQSVGQLERHFGKDGATSWIDGCAFASYAAIKALDTARNISAQCGEMTVEVQG
RSRNIGWDTKGGGTRKSESVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKEMDAQAK
ANRFVRT
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 204696..214299
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ELI29_RS30950 (ELI29_30940) | 201542..203263 | - | 1722 | WP_130685251 | ShlB/FhaC/HecB family hemolysin secretion/activation protein | - |
| ELI29_RS30955 (ELI29_30945) | 203276..203911 | - | 636 | WP_130680325 | invasion associated locus B family protein | - |
| ELI29_RS37455 | 204385..204553 | + | 169 | Protein_187 | nucleotidyltransferase | - |
| ELI29_RS30960 (ELI29_30950) | 204696..205736 | - | 1041 | WP_130671371 | P-type DNA transfer ATPase VirB11 | virB11 |
| ELI29_RS30965 (ELI29_30955) | 205743..206915 | - | 1173 | WP_130671370 | type IV secretion system protein VirB10 | virB10 |
| ELI29_RS30970 (ELI29_30960) | 206925..207779 | - | 855 | WP_130680326 | P-type conjugative transfer protein VirB9 | virB9 |
| ELI29_RS30975 (ELI29_30965) | 207776..208447 | - | 672 | WP_130680327 | virB8 family protein | virB8 |
| ELI29_RS30980 (ELI29_30970) | 208449..208733 | - | 285 | WP_130671367 | hypothetical protein | - |
| ELI29_RS30985 (ELI29_30975) | 208774..209706 | - | 933 | WP_130685253 | type IV secretion system protein | virB6 |
| ELI29_RS30990 (ELI29_30980) | 209710..209943 | - | 234 | WP_130671365 | EexN family lipoprotein | - |
| ELI29_RS30995 (ELI29_30985) | 209940..210605 | - | 666 | WP_245487114 | P-type DNA transfer protein VirB5 | virB5 |
| ELI29_RS31000 (ELI29_30990) | 210638..213004 | - | 2367 | WP_130671363 | VirB4 family type IV secretion system protein | virb4 |
| ELI29_RS31005 (ELI29_30995) | 212997..213335 | - | 339 | WP_130671362 | type IV secretion system protein VirB3 | virB3 |
| ELI29_RS31010 (ELI29_31000) | 213341..213640 | - | 300 | WP_130671361 | TrbC/VirB2 family protein | virB2 |
| ELI29_RS31015 (ELI29_31005) | 213637..214299 | - | 663 | WP_130671360 | lytic transglycosylase domain-containing protein | virB1 |
| ELI29_RS31020 (ELI29_31010) | 214303..214842 | - | 540 | WP_130685255 | hypothetical protein | - |
| ELI29_RS31025 (ELI29_31015) | 214934..215287 | + | 354 | WP_130671358 | MarR family transcriptional regulator | - |
| ELI29_RS31035 (ELI29_31025) | 215751..216726 | + | 976 | Protein_202 | UbiA prenyltransferase family protein | - |
| ELI29_RS31040 (ELI29_31030) | 216723..217427 | + | 705 | WP_130671357 | HAD family hydrolase | - |
| ELI29_RS31045 (ELI29_31035) | 217424..217789 | + | 366 | WP_130671356 | GtrA family protein | - |
| ELI29_RS31050 (ELI29_31040) | 217943..218444 | + | 502 | Protein_205 | transposase | - |
Host bacterium
| ID | 2970 | GenBank | NZ_SIQC01000004 |
| Plasmid name | pSM154C_Rh03 | Incompatibility group | - |
| Plasmid size | 313025 bp | Coordinate of oriT [Strand] | 174105..174133 [-] |
| Host baterium | Rhizobium leguminosarum strain SM154C |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |