Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102520
• Name: oriT_pSM155C_Rh02
• Organism: Rhizobium leguminosarum strain SM155C
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_SIQF01000003 (389576..389604 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSM155C_Rh02
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_ELI32_RS30540_pSM155C_Rh02

• ID: 1871
• GenBank: WP_130696272
• Name: traA_ELI32_RS30540_pSM155C_Rh02
• UniProt ID: _
• Length: 1545 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1545 a.a.
• Molecular weight: 170583.63 Da
• Isoelectric Point: 7.2557

>WP_130696272.1 Ti-type conjugative transfer relaxase TraA [Rhizobium leguminosarum]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELALPDQTPDWLRAAIDG
RSVAAASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIGLVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTTLRPLTQEGFGRKKVAVTGENGAPLRVVTPDRPTGRIVYKLWAGDKETMKAWKVAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRGAEMYFAPADLARRQEMADRLLADPALLLKQ
LSNERSTFDERDIAKALHRTVDDPLDFANIRARLMASDDLVMLKPQQVDTETGKAAEPAVFTTRDILRIE
YDMARSAQVLSERRGFAVSAESLAAAIENVETADPEKSFRLDPEQVDAVHHVTRDNGIAAVVGLAGAGKS
TLLAAARVAWESGGSRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWANGHETLHRGDVVVVDEAGMVS
SQQMARLLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQRQQWAREASRLFARGE
IEKGLDAYAQQGHLIEAGTRAETIDRIVADWSEARKRAIETAALKGNDGRLRGDELLVLAHTNQDVKRLN
EALRSVMTGEGALGENRSFQTERGGREFAAGDRIIFLENSRFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GDTLLSVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGSTVERTLVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEAKPEWGRKPRVDHTAGVTGELVETGQAKFRPNDEDADDSPYADVKTEDGSVHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVERVKVQVAIVDEQTGQKRFEEREVERNVWTAKRIETAAERQQRIERESHRP
ELFKQLVERLSRSGAKTTTLDFESEAGYQAHADDFARRRGLYTHSQTAAGMEEGVSRRWVWIAEKREQLA
ELWERASVALGFAIERERRASYNEERIETQTVPDADAGRARYLLPSTTVFARSVEEDARLAQLASPAWKE
REAILRPLLEKIYRDPDAALVALNALASDKTIEPRRLADDLAVAPDRLGRVRGSDLIVDGRAARDERSIA
IAALTELIPLARAHATEFRRNAERFGVREQQRRAHMSLPIPALSKTAMARLVEIEAVRRQGGDDAYKTAF
ALATEDRSLVQEVKAVSEALTARFGWSAFTSNADALAERNMVERMPEDLASTRREKLTRLFEAVKRFADE
QHLAERRDRSKIVAGASADQGMEAGKENAAVLPMLAAVTEFRTPIDEEARSRALATPLYRQQRTALATVA
STIWRDPAGAVGKIEDLLAKGFAGDRIAAAVTNDPAAYGALRGSDRLMDRMLAAGRERKDAVQALPEAAA
CLRALGSAYVNMLAAERHAIAEERRRMAVAIPGLSKAAEEALMRLTAEAKNNGRKPNASAVSLDPGIRRE
FAAVSTALDARFGRNAILRDDKDLINRIPPVQRRAFEAMQERFKVLQQAVRIESSEQIISERRQRAVSRG
HGIDL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• AAA domain (AAA_30) (391-576)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1045-1127)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1315-1405)

Protein structure

No available structure.

Host bacterium

• ID: 2964
• GenBank: NZ_SIQF01000003
• Plasmid name: pSM155C_Rh02
• Incompatibility group: -
• Plasmid size: 480486 bp
• Coordinate of oriT [Strand]: 389576..389604 [+]
• Host baterium: Rhizobium leguminosarum strain SM155C

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA9