Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102504 |
| Name | oriT_pCRKP-31_Vir  |
| Organism | Klebsiella pneumoniae strain CRKP-31 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JANPXP010000004 (131406..131433 [+], 28 nt) |
| oriT length | 28 nt |
| IRs (inverted repeats) | 16..21, 23..28 (ATCAGA..TCTGAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 28 nt
>oriT_pCRKP-31_Vir
AGTTTGGTGCTTATGATCAGAATCTGAT
AGTTTGGTGCTTATGATCAGAATCTGAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1454 | GenBank | WP_032438003 |
| Name | traD_NVV53_RS01080_pCRKP-31_Vir |
UniProt ID | _ |
| Length | 708 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 708 a.a. Molecular weight: 79542.03 Da Isoelectric Point: 8.6444
>WP_032438003.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MKRRSRNNLHTQEMLYRPALEFRSALILILFSAYVLIDSWTSKYGISEIPFYCSLGLIAMAGWRVWHGVP
VFIAHWRLFHRTMDFLSLEDFRMINNAHFFADDRKYQKLVSLQESGGAPSKKSVYKLLRKKEKIVIPQRT
TFLCNGFKWGPEHSERTYQVHNLSSDMHEVQLPFILNPVTRHYRKLAFDLGGNYAIFGVDKKIPIFVNEE
NFFGHTLITGNVGTGKTVLQRLLSSSMLHLGHIVLVVDPKNDYQWQEGLKEECESLGKPFMHFHAGNPST
SVSYDVSANYVKDTDLSARIMSIISGTEGGEDPFVRIAEGLVTTAIGALKLGGTKPTIQNIYYAIRSKQD
LFVTTRNALRGFYTYHLGADWHLTVQVSPNLTFADEIEKLKEYFHCNYFEDNSPKNMHGMDTVLECFKYI
SGDESHYYKITASLMPMLKRLSQTPMDILLSANDVENPERNIVNSHGLFNSGGVLYISLDGLSDPATARD
LSQLITSDIAAEAGSRYNTASDLSTVPRVSIFIDEAHQAVNMQLINLLAQGRAAKIALFISTQTISDFVS
ATSADTADRLTGLCNNYISTRVTDAKTQELVLTKVGQTNVSMNQVTYTTSAGTKQSHTDFNGSISERKST
TMVNAIPQELLSMIPTLHFIACLQDGRKIVGQMPITVPGKSMRRSTTVFDMVTTSPYKLKMRRNLNVEEI
LSKSQKVG
MKRRSRNNLHTQEMLYRPALEFRSALILILFSAYVLIDSWTSKYGISEIPFYCSLGLIAMAGWRVWHGVP
VFIAHWRLFHRTMDFLSLEDFRMINNAHFFADDRKYQKLVSLQESGGAPSKKSVYKLLRKKEKIVIPQRT
TFLCNGFKWGPEHSERTYQVHNLSSDMHEVQLPFILNPVTRHYRKLAFDLGGNYAIFGVDKKIPIFVNEE
NFFGHTLITGNVGTGKTVLQRLLSSSMLHLGHIVLVVDPKNDYQWQEGLKEECESLGKPFMHFHAGNPST
SVSYDVSANYVKDTDLSARIMSIISGTEGGEDPFVRIAEGLVTTAIGALKLGGTKPTIQNIYYAIRSKQD
LFVTTRNALRGFYTYHLGADWHLTVQVSPNLTFADEIEKLKEYFHCNYFEDNSPKNMHGMDTVLECFKYI
SGDESHYYKITASLMPMLKRLSQTPMDILLSANDVENPERNIVNSHGLFNSGGVLYISLDGLSDPATARD
LSQLITSDIAAEAGSRYNTASDLSTVPRVSIFIDEAHQAVNMQLINLLAQGRAAKIALFISTQTISDFVS
ATSADTADRLTGLCNNYISTRVTDAKTQELVLTKVGQTNVSMNQVTYTTSAGTKQSHTDFNGSISERKST
TMVNAIPQELLSMIPTLHFIACLQDGRKIVGQMPITVPGKSMRRSTTVFDMVTTSPYKLKMRRNLNVEEI
LSKSQKVG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 243764..252797
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NVV53_RS02055 (NVV53_02050) | 238766..239170 | - | 405 | WP_032437918 | hypothetical protein | - |
| NVV53_RS02060 (NVV53_02055) | 239145..239603 | - | 459 | WP_004196672 | hypothetical protein | - |
| NVV53_RS02065 (NVV53_02060) | 240287..241090 | + | 804 | WP_014386512 | metallophosphoesterase | - |
| NVV53_RS02070 (NVV53_02065) | 241209..242177 | + | 969 | WP_161400566 | IS5-like element IS903 family transposase | - |
| NVV53_RS02075 (NVV53_02070) | 242254..242862 | + | 609 | WP_232152184 | hypothetical protein | - |
| NVV53_RS02080 (NVV53_02075) | 242849..243349 | + | 501 | WP_004196661 | hypothetical protein | - |
| NVV53_RS02085 (NVV53_02080) | 243321..243737 | + | 417 | WP_014386510 | hypothetical protein | - |
| NVV53_RS02090 (NVV53_02085) | 243764..246487 | - | 2724 | WP_163595835 | TraC family protein | virb4 |
| NVV53_RS02095 (NVV53_02090) | 246532..247269 | - | 738 | WP_004181797 | type IV conjugative transfer system lipoprotein TraV | traV |
| NVV53_RS02100 (NVV53_02095) | 247279..248130 | - | 852 | WP_025368610 | disulfide isomerase | - |
| NVV53_RS02105 (NVV53_02100) | 248133..248597 | - | 465 | WP_004181799 | hypothetical protein | - |
| NVV53_RS02110 (NVV53_02105) | 248600..249970 | - | 1371 | WP_032437928 | TrbI/VirB10 family protein | traB |
| NVV53_RS02115 (NVV53_02110) | 249930..250448 | - | 519 | WP_014386508 | hypothetical protein | - |
| NVV53_RS02120 (NVV53_02115) | 250445..251614 | - | 1170 | WP_004026506 | type-F conjugative transfer system secretin TraK | traK |
| NVV53_RS02125 (NVV53_02120) | 251616..252476 | - | 861 | WP_004196647 | TraE/TraK family type IV conjugative transfer system protein | traE |
| NVV53_RS02130 (NVV53_02125) | 252495..252797 | - | 303 | WP_024198097 | type IV conjugative transfer system protein TraL | traL |
| NVV53_RS02135 (NVV53_02130) | 252899..253267 | - | 369 | WP_004026504 | hypothetical protein | - |
| NVV53_RS02140 (NVV53_02135) | 254083..254397 | + | 315 | WP_032437932 | hypothetical protein | - |
| NVV53_RS02145 (NVV53_02140) | 254542..255210 | + | 669 | WP_014386506 | hypothetical protein | - |
| NVV53_RS02150 (NVV53_02145) | 255266..256030 | + | 765 | WP_070083074 | hypothetical protein | - |
| NVV53_RS02155 (NVV53_02150) | 256158..257336 | + | 1179 | WP_004196657 | hypothetical protein | - |
| NVV53_RS02160 (NVV53_02155) | 257378..257506 | + | 129 | WP_004196653 | hypothetical protein | - |
Host bacterium
| ID | 2948 | GenBank | NZ_JANPXP010000004 |
| Plasmid name | pCRKP-31_Vir | Incompatibility group | IncFIB |
| Plasmid size | 291810 bp | Coordinate of oriT [Strand] | 131406..131433 [+] |
| Host baterium | Klebsiella pneumoniae strain CRKP-31 |
Cargo genes
| Drug resistance gene | mph(E), msr(E), armA, sul1, blaDHA-1, qnrB4 |
| Virulence gene | iucA, iucB, iucC, iutA, rmpA |
| Metal resistance gene | terE, terD, terC, terB, terA, terZ, terW, merR, merT, merP, merC |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |