Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102483
Name   oriT_INSLA289|unnamed INSLA289_contig_77 in_silico
Organism   Escherichia coli strain INSLA289
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LHAT01000077 (1353..1441 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_INSLA289|unnamed INSLA289_contig_77
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1848 GenBank   WP_024143648
Name   nikB_AKG99_RS23575_INSLA289|unnamed INSLA289_contig_77 insolico UniProt ID   A0A023JAU0
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_024143648.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNNDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A023JAU0


Auxiliary protein

ID   763 GenBank   WP_001283947
Name   WP_001283947_INSLA289|unnamed INSLA289_contig_77 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1439 GenBank   WP_001289276
Name   trbC_AKG99_RS23580_INSLA289|unnamed INSLA289_contig_77 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 6819..36249

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKG99_RS23580 (AKG99_23650) 4535..6826 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
AKG99_RS23585 (AKG99_23655) 6819..7889 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
AKG99_RS23590 (AKG99_23660) 7908..9116 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
AKG99_RS23595 (AKG99_23665) 9408..9560 + 153 WP_001331364 Hok/Gef family protein -
AKG99_RS23600 (AKG99_23670) 9632..9883 - 252 WP_001291964 hypothetical protein -
AKG99_RS33890 (AKG99_23675) 10382..10477 + 96 WP_001303310 DinQ-like type I toxin DqlB -
AKG99_RS32805 (AKG99_23680) 10542..10718 - 177 WP_001054904 hypothetical protein -
AKG99_RS23615 (AKG99_23690) 11110..11319 + 210 WP_000062603 HEAT repeat domain-containing protein -
AKG99_RS23620 (AKG99_23695) 11391..12041 - 651 WP_001178506 plasmid IncI1-type surface exclusion protein ExcA -
AKG99_RS23625 (AKG99_23700) 12115..14283 - 2169 WP_021537350 DotA/TraY family protein traY
AKG99_RS23630 (AKG99_23705) 14380..14964 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
AKG99_RS23635 (AKG99_23710) 14993..16195 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
AKG99_RS23640 (AKG99_23715) 16162..16776 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
AKG99_RS23645 (AKG99_23720) 16776..19820 - 3045 WP_021265678 IncI1-type conjugal transfer protein TraU traU
AKG99_RS23650 (AKG99_23725) 19910..20710 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
AKG99_RS23655 (AKG99_23730) 20694..20882 - 189 WP_001277255 putative conjugal transfer protein TraS -
AKG99_RS23660 (AKG99_23735) 20946..21350 - 405 WP_000086958 IncI1-type conjugal transfer protein TraR traR
AKG99_RS23665 (AKG99_23740) 21401..21928 - 528 WP_001055569 conjugal transfer protein TraQ traQ
AKG99_RS23670 (AKG99_23745) 21928..22632 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
AKG99_RS23675 (AKG99_23750) 22632..23921 - 1290 WP_001272003 conjugal transfer protein TraO traO
AKG99_RS23680 (AKG99_23755) 23924..24907 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
AKG99_RS23685 (AKG99_23760) 24918..25610 - 693 WP_000138550 DotI/IcmL family type IV secretion protein traM
AKG99_RS23690 (AKG99_23765) 25607..25954 - 348 WP_001055900 conjugal transfer protein traL
AKG99_RS23695 (AKG99_23770) 25972..29739 - 3768 WP_001141527 LPD7 domain-containing protein -
AKG99_RS23700 (AKG99_23775) 29828..30379 - 552 WP_000014583 phospholipase D family protein -
AKG99_RS30265 30394..30684 - 291 WP_001299214 hypothetical protein traK
AKG99_RS23705 (AKG99_23780) 30681..31829 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
AKG99_RS23710 (AKG99_23785) 31826..32644 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
AKG99_RS23715 (AKG99_23790) 32641..33099 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
AKG99_RS23720 (AKG99_23795) 33494..34078 - 585 WP_000977522 histidine phosphatase family protein -
AKG99_RS23725 (AKG99_23800) 34138..35340 - 1203 WP_000976351 conjugal transfer protein TraF -
AKG99_RS23730 (AKG99_23805) 35425..36249 - 825 WP_001238939 conjugal transfer protein TraE traE
AKG99_RS23735 (AKG99_23810) 36400..37554 - 1155 WP_001139957 site-specific integrase -
AKG99_RS23740 (AKG99_23815) 38349..39611 + 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
AKG99_RS23750 (AKG99_23825) 39899..40774 + 876 WP_013188473 extended-spectrum class A beta-lactamase CTX-M-1 -
AKG99_RS23755 (AKG99_23830) 40821..41165 - 345 Protein_39 WbuC family cupin fold metalloprotein -

Host bacterium

ID   2927 GenBank   NZ_LHAT01000077
Plasmid name   INSLA289|unnamed INSLA289_contig_77 Incompatibility group   -
Plasmid size   41666 bp Coordinate of oriT [Strand]   1353..1441 [-]
Host baterium   Escherichia coli strain INSLA289

Cargo genes

Drug resistance gene   blaCTX-M-1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -