Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102452
Name   oriT_148|unnamed15 in_silico
Organism   Klebsiella pneumoniae strain 148
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LZCY01000051 (31610..31659 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_148|unnamed15
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1833 GenBank   WP_065898399
Name   traI_A9Z49_RS27705_148|unnamed15 insolico UniProt ID   _
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190718.76 Da        Isoelectric Point: 6.2077

>WP_065898399.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-284)

DNA helicase TraI (TraI_C)

(1433-1555)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

AAA domain (AAA_30)

(971-1157)


  Protein structure



No available structure.




T4CP

ID   1423 GenBank   WP_004152630
Name   traD_A9Z49_RS27700_148|unnamed15 insolico UniProt ID   _
Length   769 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 769 a.a.        Molecular weight: 85951.02 Da        Isoelectric Point: 5.0632

>WP_004152630.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 34163..59470

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9Z49_RS27520 (A9Z49_28335) 30324..31292 + 969 WP_011977741 IS5-like element IS903B family transposase -
A9Z49_RS27525 (A9Z49_28340) 31349..31537 - 189 Protein_33 transglycosylase SLT domain-containing protein -
A9Z49_RS27530 (A9Z49_28345) 31971..32363 + 393 WP_004178062 conjugal transfer relaxosome DNA-binding protein TraM -
A9Z49_RS27535 (A9Z49_28350) 32577..33272 + 696 WP_004178061 transcriptional regulator TraJ family protein -
A9Z49_RS27540 (A9Z49_28355) 33356..33727 + 372 WP_004208838 TraY domain-containing protein -
A9Z49_RS27545 (A9Z49_28360) 33781..34149 + 369 WP_004178060 type IV conjugative transfer system pilin TraA -
A9Z49_RS27550 (A9Z49_28365) 34163..34468 + 306 WP_004178059 type IV conjugative transfer system protein TraL traL
A9Z49_RS27555 (A9Z49_28370) 34488..35054 + 567 WP_004152602 type IV conjugative transfer system protein TraE traE
A9Z49_RS27560 (A9Z49_28375) 35041..35781 + 741 WP_004152601 type-F conjugative transfer system secretin TraK traK
A9Z49_RS27565 (A9Z49_28380) 35781..37205 + 1425 WP_004152600 F-type conjugal transfer pilus assembly protein TraB traB
A9Z49_RS27570 (A9Z49_28385) 37198..37794 + 597 WP_004152599 conjugal transfer pilus-stabilizing protein TraP -
A9Z49_RS27575 (A9Z49_28390) 37817..38386 + 570 WP_004152598 type IV conjugative transfer system lipoprotein TraV traV
A9Z49_RS27580 (A9Z49_28395) 38518..38928 + 411 WP_004152597 lipase chaperone -
A9Z49_RS27585 (A9Z49_28400) 38933..39223 + 291 WP_004152596 hypothetical protein -
A9Z49_RS27590 (A9Z49_28405) 39247..39465 + 219 WP_004171484 hypothetical protein -
A9Z49_RS27595 (A9Z49_28410) 39466..39783 + 318 WP_004152595 hypothetical protein -
A9Z49_RS27600 (A9Z49_28415) 39850..40254 + 405 WP_004152594 hypothetical protein -
A9Z49_RS27610 (A9Z49_28425) 40550..40948 + 399 WP_004153071 hypothetical protein -
A9Z49_RS27615 (A9Z49_28430) 41020..43659 + 2640 WP_004152592 type IV secretion system protein TraC virb4
A9Z49_RS27620 (A9Z49_28435) 43659..44048 + 390 WP_004152591 type-F conjugative transfer system protein TrbI -
A9Z49_RS27625 (A9Z49_28440) 44048..44674 + 627 WP_004152590 type-F conjugative transfer system protein TraW traW
A9Z49_RS27630 (A9Z49_28445) 44718..45677 + 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
A9Z49_RS27635 (A9Z49_28450) 45690..46328 + 639 WP_004152682 type-F conjugative transfer system pilin assembly protein TrbC trbC
A9Z49_RS27640 (A9Z49_28455) 46376..48331 + 1956 WP_004153093 type-F conjugative transfer system mating-pair stabilization protein TraN traN
A9Z49_RS27645 (A9Z49_28460) 48363..48599 + 237 WP_004152683 conjugal transfer protein TrbE -
A9Z49_RS29815 (A9Z49_28465) 48596..48781 + 186 WP_004152684 hypothetical protein -
A9Z49_RS27655 (A9Z49_28470) 48827..49153 + 327 WP_004152685 hypothetical protein -
A9Z49_RS27660 (A9Z49_28475) 49174..49926 + 753 WP_004152686 type-F conjugative transfer system pilin assembly protein TraF traF
A9Z49_RS27665 (A9Z49_28480) 49937..50176 + 240 WP_004152687 type-F conjugative transfer system pilin chaperone TraQ -
A9Z49_RS27670 (A9Z49_28485) 50148..50720 + 573 WP_004152688 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
A9Z49_RS27675 (A9Z49_28490) 50713..51141 + 429 WP_004152689 hypothetical protein -
A9Z49_RS27680 (A9Z49_28495) 51128..52498 + 1371 WP_004178055 conjugal transfer pilus assembly protein TraH traH
A9Z49_RS27685 (A9Z49_28500) 52498..55347 + 2850 WP_004178054 conjugal transfer mating-pair stabilization protein TraG traG
A9Z49_RS27690 (A9Z49_28505) 55353..55880 + 528 WP_004153030 hypothetical protein -
A9Z49_RS27695 (A9Z49_28510) 56070..56801 + 732 WP_004152629 conjugal transfer complement resistance protein TraT -
A9Z49_RS27700 (A9Z49_28515) 57161..59470 + 2310 WP_004152630 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   2896 GenBank   NZ_LZCY01000051
Plasmid name   148|unnamed15 Incompatibility group   IncFII
Plasmid size   94582 bp Coordinate of oriT [Strand]   31610..31659 [-]
Host baterium   Klebsiella pneumoniae strain 148

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   fecD, fecE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -