Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102449
Name   oriT_SMG017F8|unnamed1 in_silico
Organism   Escherichia coli strain SMG017F8
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JALECD010000023 (90469..90541 [-], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_SMG017F8|unnamed1
GTCGGGGCAAAGCCCTGACCAGGTGGCATTTGTCTGATTGCGCGTGCGCGGTCCGACAAATGCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1832 GenBank   WP_001681714
Name   Relaxase_MRT19_RS19830_SMG017F8|unnamed1 insolico UniProt ID   S1QCP9
Length   903 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 903 a.a.        Molecular weight: 104574.90 Da        Isoelectric Point: 7.6304

>WP_001681714.1 relaxase/mobilization nuclease domain-containing protein [Escherichia coli]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPVSGHINRLSWSQERLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRYFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
TTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGS
VRFRDRRNGELVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAW
HNAAGITGNGHFVISRPDVDLHRQRSEQYYHEYIRQQKSISGGNDTSYTPVQDNEWTPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure


Source ID Structure
AlphaFold DB S1QCP9


Auxiliary protein

ID   751 GenBank   WP_001291058
Name   WP_001291058_SMG017F8|unnamed1 insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   1422 GenBank   WP_001760655
Name   trbC_MRT19_RS19295_SMG017F8|unnamed1 insolico UniProt ID   _
Length   767 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 767 a.a.        Molecular weight: 87070.28 Da        Isoelectric Point: 7.4857

>WP_001760655.1 F-type conjugative transfer protein TrbC [Escherichia coli]
MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIWPLALPVCLLFTLVTSVIFMLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVM
FFATTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMLLARDLGYRLE
GTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSGGKQESRKKRF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3325..45532

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MRT19_RS19285 (MRT19_19310) 1..224 + 224 Protein_0 IS66 family insertion sequence element accessory protein TnpB -
MRT19_RS19290 (MRT19_19315) 344..574 - 231 WP_065406859 hypothetical protein -
MRT19_RS19295 (MRT19_19320) 1041..3344 - 2304 WP_001760655 F-type conjugative transfer protein TrbC -
MRT19_RS19300 (MRT19_19325) 3325..4449 - 1125 WP_000152385 DsbC family protein trbB
MRT19_RS19305 (MRT19_19330) 4446..5780 - 1335 WP_021529215 IncI1-type conjugal transfer protein TrbA trbA
MRT19_RS27300 6279..6377 + 99 WP_001681653 DinQ-like type I toxin DqlB -
MRT19_RS19310 (MRT19_19335) 6437..6613 - 177 WP_001054907 hypothetical protein -
MRT19_RS19315 (MRT19_19340) 6764..7231 - 468 WP_001681654 thermonuclease family protein -
MRT19_RS19320 (MRT19_19345) 7396..8019 + 624 WP_024187057 helix-turn-helix domain-containing protein -
MRT19_RS19325 (MRT19_19350) 8217..8429 - 213 WP_000264905 hypothetical protein -
MRT19_RS19330 (MRT19_19355) 8417..8800 - 384 WP_001681657 hypothetical protein -
MRT19_RS19335 (MRT19_19360) 8812..9003 - 192 WP_024187058 hypothetical protein -
MRT19_RS19340 (MRT19_19365) 9099..9341 - 243 WP_000650933 hypothetical protein -
MRT19_RS19345 (MRT19_19370) 9670..9822 + 153 WP_001302699 Hok/Gef family protein -
MRT19_RS19350 (MRT19_19375) 9960..11546 - 1587 WP_000004564 TnsA endonuclease N-terminal domain-containing protein -
MRT19_RS19355 (MRT19_19380) 11820..12467 - 648 WP_180399448 plasmid IncI1-type surface exclusion protein ExcA -
MRT19_RS19360 (MRT19_19385) 12555..14714 - 2160 WP_012605167 DotA/TraY family protein traY
MRT19_RS19365 (MRT19_19390) 14789..15358 - 570 WP_021561196 conjugal transfer protein TraX -
MRT19_RS19370 (MRT19_19395) 15355..16560 - 1206 WP_180399447 conjugal transfer protein TraW traW
MRT19_RS19375 (MRT19_19400) 16518..17138 - 621 WP_000286854 hypothetical protein traV
MRT19_RS19380 (MRT19_19405) 17138..20182 - 3045 WP_025672387 conjugal transfer protein traU
MRT19_RS19385 (MRT19_19410) 20358..20738 - 381 WP_180399451 hypothetical protein -
MRT19_RS19390 (MRT19_19415) 20738..21097 - 360 WP_016238180 hypothetical protein -
MRT19_RS19395 (MRT19_19420) 21152..21970 - 819 WP_148722550 conjugal transfer protein TraT traT
MRT19_RS27305 21885..22136 - 252 WP_001277573 hypothetical protein -
MRT19_RS19405 (MRT19_19430) 22193..22591 - 399 WP_000088813 DUF6750 family protein traR
MRT19_RS19410 (MRT19_19435) 22638..23168 - 531 WP_000987023 conjugal transfer protein TraQ traQ
MRT19_RS19415 (MRT19_19440) 23165..23878 - 714 WP_000787000 conjugal transfer protein TraP traP
MRT19_RS19420 (MRT19_19445) 23875..25206 - 1332 WP_021561193 conjugal transfer protein TraO traO
MRT19_RS19425 (MRT19_19450) 25210..26184 - 975 WP_000547383 DotH/IcmK family type IV secretion protein traN
MRT19_RS19430 (MRT19_19455) 26195..26890 - 696 WP_001681668 DotI/IcmL family type IV secretion protein traM
MRT19_RS19435 (MRT19_19460) 26902..27252 - 351 WP_001056367 hypothetical protein traL
MRT19_RS19440 (MRT19_19465) 27269..31330 - 4062 WP_021561192 LPD7 domain-containing protein -
MRT19_RS19445 (MRT19_19470) 31684..32265 - 582 WP_021561191 hypothetical protein -
MRT19_RS19450 (MRT19_19475) 32497..32787 - 291 WP_000817801 IcmT/TraK family protein traK
MRT19_RS19455 (MRT19_19480) 32784..33932 - 1149 WP_021565170 plasmid transfer ATPase TraJ virB11
MRT19_RS19460 (MRT19_19485) 33916..34752 - 837 WP_012605182 type IV secretory system conjugative DNA transfer family protein traI
MRT19_RS19465 (MRT19_19490) 34749..35207 - 459 WP_001170111 DotD/TraH family lipoprotein -
MRT19_RS19470 (MRT19_19495) 35311..36513 - 1203 WP_000979993 conjugal transfer protein TraF -
MRT19_RS19475 (MRT19_19500) 36615..37436 - 822 WP_001681675 hypothetical protein traE
MRT19_RS19480 (MRT19_19505) 37698..38036 + 339 WP_001681677 hypothetical protein -
MRT19_RS19485 (MRT19_19510) 38323..39447 - 1125 WP_089502979 site-specific integrase -
MRT19_RS19490 (MRT19_19515) 39769..41142 - 1374 WP_180399446 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MRT19_RS19495 (MRT19_19520) 41139..41771 - 633 WP_000152530 A24 family peptidase -
MRT19_RS19500 (MRT19_19525) 41806..42288 - 483 WP_021561187 lytic transglycosylase domain-containing protein virB1
MRT19_RS19505 (MRT19_19530) 42336..42872 - 537 WP_001011500 type 4 pilus major pilin -
MRT19_RS19510 (MRT19_19535) 42922..44022 - 1101 WP_001211427 type II secretion system F family protein -
MRT19_RS19515 (MRT19_19540) 44024..45532 - 1509 WP_012605185 ATPase, T2SS/T4P/T4SS family virB11
MRT19_RS19520 (MRT19_19545) 45723..45974 + 252 WP_001681796 hypothetical protein -
MRT19_RS19525 (MRT19_19550) 45964..46245 + 282 WP_001681795 type II toxin-antitoxin system RelE/ParE family toxin -
MRT19_RS19530 (MRT19_19555) 46284..46502 - 219 WP_175125356 hypothetical protein -
MRT19_RS19535 (MRT19_19560) 46555..47007 - 453 WP_016238167 type IV pilus biogenesis protein PilP -
MRT19_RS19540 (MRT19_19565) 46997..48292 - 1296 WP_032325932 type 4b pilus protein PilO2 -
MRT19_RS19545 (MRT19_19570) 48313..49932 - 1620 WP_021561186 PilN family type IVB pilus formation outer membrane protein -
MRT19_RS19550 (MRT19_19575) 49963..50400 - 438 WP_001681791 type IV pilus biogenesis protein PilM -

Host bacterium

ID   2893 GenBank   NZ_JALECD010000023
Plasmid name   SMG017F8|unnamed1 Incompatibility group   IncB/O/K/Z
Plasmid size   101286 bp Coordinate of oriT [Strand]   90469..90541 [-]
Host baterium   Escherichia coli strain SMG017F8

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -