Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102432 |
| Name | oriT_pCZ863_IncM1  |
| Organism | Enterobacter asburiae strain CZ863 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAGSXX010000006 (50278..50383 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pCZ863_IncM1
AGTACGGAACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGAACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1819 | GenBank | WP_059509498 |
| Name | Relaxase_KD033_RS23545_pCZ863_IncM1 |
UniProt ID | A0A0E3MRA0 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75193.92 Da Isoelectric Point: 9.9948
>WP_059509498.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQIFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQIFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E3MRA0 |
T4CP
| ID | 1408 | GenBank | WP_004187443 |
| Name | t4cp2_KD033_RS23210_pCZ863_IncM1 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79519.87 Da Isoelectric Point: 4.8309
>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 53179..72372
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KD033_RS23525 (KD033_23525) | 48264..48575 | + | 312 | WP_004187333 | hypothetical protein | - |
| KD033_RS23530 (KD033_23530) | 48708..49244 | + | 537 | WP_011091066 | hypothetical protein | - |
| KD033_RS23535 (KD033_23535) | 49745..50140 | - | 396 | WP_211959056 | MobC | - |
| KD033_RS23880 | 50175..50702 | + | 528 | WP_224253863 | plasmid mobilization protein MobA | - |
| KD033_RS23545 (KD033_23545) | 50689..52668 | + | 1980 | WP_059509498 | TraI/MobA(P) family conjugative relaxase | - |
| KD033_RS23550 (KD033_23550) | 52682..53182 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| KD033_RS23555 (KD033_23555) | 53179..53958 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| KD033_RS23560 (KD033_23560) | 53969..55132 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| KD033_RS23565 (KD033_23565) | 55122..55382 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| KD033_RS23885 | 55407..58709 | + | 3303 | WP_031942492 | LPD7 domain-containing protein | - |
| KD033_RS23575 (KD033_23575) | 58675..59187 | + | 513 | WP_011091071 | hypothetical protein | traL |
| KD033_RS23580 (KD033_23580) | 59188..59400 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| KD033_RS23585 (KD033_23585) | 59372..59782 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| KD033_RS23590 (KD033_23590) | 59850..60563 | + | 714 | WP_109914255 | DotI/IcmL family type IV secretion protein | traM |
| KD033_RS23595 (KD033_23595) | 60572..61723 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| KD033_RS23600 (KD033_23600) | 61735..63084 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| KD033_RS23605 (KD033_23605) | 63096..63800 | + | 705 | WP_031942491 | conjugal transfer protein TraP | traP |
| KD033_RS23610 (KD033_23610) | 63824..64354 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| KD033_RS23615 (KD033_23615) | 64371..64760 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| KD033_RS23620 (KD033_23620) | 64806..65300 | + | 495 | WP_011091080 | hypothetical protein | - |
| KD033_RS23625 (KD033_23625) | 65297..68347 | + | 3051 | WP_117052919 | conjugal transfer protein | traU |
| KD033_RS23630 (KD033_23630) | 68377..69552 | + | 1176 | WP_167577398 | conjugal transfer protein TraW | traW |
| KD033_RS23635 (KD033_23635) | 69549..70199 | + | 651 | WP_004187488 | hypothetical protein | - |
| KD033_RS23640 (KD033_23640) | 70192..72372 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
Host bacterium
| ID | 2876 | GenBank | NZ_JAGSXX010000006 |
| Plasmid name | pCZ863_IncM1 | Incompatibility group | IncL/M |
| Plasmid size | 72524 bp | Coordinate of oriT [Strand] | 50278..50383 [+] |
| Host baterium | Enterobacter asburiae strain CZ863 |
Cargo genes
| Drug resistance gene | blaTEM-1D, blaOXA-1, aac(6')-Ib-cr |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |