Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   102362
Name   oriT_Res13-Croi-PEB01-41|unnamed466 in_silico
Organism   Escherichia fergusonii strain Res13-Croi-PEB01-41
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JADAID010000136 (4202..4376 [-], 175 nt)
oriT length   175 nt
IRs (inverted repeats)      101..106, 120..125  (ACAATC..GATTGT)
 46..51, 54..59  (CAGTTT..AAACTG)
Location of nic site      65..66
Conserved sequence flanking the
  nic site  
 
 ACTGGCATAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 175 nt

>oriT_Res13-Croi-PEB01-41|unnamed466
GTAACTCCGTGCGCGATGAAAAATCGTATTTTATCGCGTCACTGGCAGTTTAAAAACTGAACTGGCATAATGCACGGCACATCACGAAGTGCGCACTTATACAATCTCCACTCCGTTTCGATTGTGTGCGCCCTGCGGGGCTGAAAGAAAACGACAAAAAGGCATTACGGCAGAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   1786 GenBank   WP_032347464
Name   mobQ_IMO57_RS24015_Res13-Croi-PEB01-41|unnamed466 insolico UniProt ID   A0A0F7JFN2
Length   719 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 719 a.a.        Molecular weight: 81786.41 Da        Isoelectric Point: 8.9615

>WP_032347464.1 MULTISPECIES: MobQ family relaxase [Enterobacteriaceae]
MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRKSGVAEAVILTPATAPAWCTNRAELW
NAVEKAERRKNSQLAREIELAIPRELPQDAARETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTT
RAVGPAGFGGKVRDWNDRTHAEAWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAM
ETRGIETERGEQNRLINRLNLEIQISRTELRNGGLTEPARRVANLLNIGLPDNATSYTLRDIIEALPKDS
NAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWAAPEYRSEQGK
IQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWAASGLAALRAQLLKREEELRRKEQEDTERSRAE
RRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNWEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDL
MRDRSGYICDRSEIGQHPPLQRVYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSL
AARKVDFTQGRLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPTADNSQLVINA
LTDKGLRFAGYGEAVNHDADSPARPAPELMLFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEWQR
EEYARTRVSDRKHERGWLL

  Protein domains


Predicted by InterproScan.

(18-213)


  Protein structure


Source ID Structure
AlphaFold DB A0A0F7JFN2


Host bacterium


ID   2806 GenBank   NZ_JADAID010000136
Plasmid name   Res13-Croi-PEB01-41|unnamed466 Incompatibility group   Col8282
Plasmid size   5093 bp Coordinate of oriT [Strand]   4202..4376 [-]
Host baterium   Escherichia fergusonii strain Res13-Croi-PEB01-41

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -