Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102356 |
| Name | oriT_FWSEC0138|unnamed3  |
| Organism | Escherichia coli strain FWSEC0138 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_RRHD01000154 (3618..3670 [+], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_FWSEC0138|unnamed3
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1367 | GenBank | WP_000338972 |
| Name | t4cp2_C9Z88_RS27070_FWSEC0138|unnamed3 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73329.86 Da Isoelectric Point: 9.1391
>WP_000338972.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDISLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDISLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 22150..45155
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C9Z88_RS26930 (C9Z88_26965) | 17642..18094 | + | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
| C9Z88_RS26935 (C9Z88_26970) | 18087..18281 | + | 195 | WP_001127358 | DUF1187 family protein | - |
| C9Z88_RS26940 (C9Z88_26975) | 18327..19280 | + | 954 | WP_072097371 | SPFH domain-containing protein | - |
| C9Z88_RS26945 (C9Z88_26980) | 19342..19785 | + | 444 | WP_000498521 | NfeD family protein | - |
| C9Z88_RS27445 | 19789..19959 | + | 171 | WP_000550721 | hypothetical protein | - |
| C9Z88_RS26950 (C9Z88_26985) | 19970..20632 | + | 663 | WP_001243159 | hypothetical protein | - |
| C9Z88_RS26960 (C9Z88_26995) | 20783..21376 | + | 594 | WP_001243171 | hypothetical protein | - |
| C9Z88_RS26970 (C9Z88_27005) | 21590..21892 | + | 303 | WP_000189499 | hypothetical protein | - |
| C9Z88_RS26975 (C9Z88_27010) | 21889..22146 | + | 258 | WP_000739144 | hypothetical protein | - |
| C9Z88_RS26980 (C9Z88_27015) | 22150..23145 | + | 996 | WP_000276068 | type IV secretion system protein | virB6 |
| C9Z88_RS26985 (C9Z88_27020) | 23151..23792 | + | 642 | WP_001415805 | type IV secretion system protein | - |
| C9Z88_RS26990 (C9Z88_27025) | 23795..24049 | + | 255 | WP_000609210 | EexN family lipoprotein | - |
| C9Z88_RS26995 (C9Z88_27030) | 24102..24911 | - | 810 | WP_001005154 | DUF5710 domain-containing protein | - |
| C9Z88_RS27000 (C9Z88_27035) | 24959..25594 | + | 636 | WP_000835769 | hypothetical protein | - |
| C9Z88_RS27005 (C9Z88_27040) | 25766..26053 | + | 288 | WP_001326593 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| C9Z88_RS27010 (C9Z88_27045) | 26056..27291 | + | 1236 | WP_000733393 | toxin co-regulated pilus biosynthesis Q family protein | - |
| C9Z88_RS27015 (C9Z88_27050) | 27297..27734 | + | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| C9Z88_RS27020 (C9Z88_27055) | 27853..28251 | + | 399 | WP_001153669 | hypothetical protein | - |
| C9Z88_RS27025 (C9Z88_27060) | 28272..28856 | + | 585 | WP_001177114 | lytic transglycosylase domain-containing protein | virB1 |
| C9Z88_RS27565 | 28856..29146 | + | 291 | WP_000865478 | TrbC/VirB2 family protein | virB2 |
| C9Z88_RS27035 (C9Z88_27070) | 29217..29537 | + | 321 | WP_000362083 | VirB3 family type IV secretion system protein | virB3 |
| C9Z88_RS27040 (C9Z88_27075) | 29543..31900 | + | 2358 | WP_000548951 | VirB4 family type IV secretion system protein | virb4 |
| C9Z88_RS27050 (C9Z88_27085) | 32066..32800 | + | 735 | WP_000432283 | type IV secretion system protein | virB8 |
| C9Z88_RS27055 (C9Z88_27090) | 32866..33567 | + | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| C9Z88_RS27060 (C9Z88_27095) | 33557..34696 | + | 1140 | WP_000790639 | TrbI/VirB10 family protein | virB10 |
| C9Z88_RS27065 (C9Z88_27100) | 34779..35834 | + | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| C9Z88_RS27070 (C9Z88_27105) | 35850..37808 | + | 1959 | WP_000338972 | type IV secretory system conjugative DNA transfer family protein | - |
| C9Z88_RS27075 (C9Z88_27110) | 37859..39502 | + | 1644 | WP_001035589 | PilN family type IVB pilus formation outer membrane protein | - |
| C9Z88_RS27080 (C9Z88_27115) | 39553..40863 | + | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| C9Z88_RS27085 (C9Z88_27120) | 40847..41341 | + | 495 | WP_000912554 | type IV pilus biogenesis protein PilP | - |
| C9Z88_RS27090 (C9Z88_27125) | 41366..42904 | + | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| C9Z88_RS27095 (C9Z88_27130) | 42895..44004 | + | 1110 | WP_000974903 | type II secretion system F family protein | - |
| C9Z88_RS27100 (C9Z88_27135) | 44049..44606 | + | 558 | WP_000095048 | type 4 pilus major pilin | - |
| C9Z88_RS27105 (C9Z88_27140) | 44673..45155 | + | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| C9Z88_RS27110 (C9Z88_27145) | 45159..45794 | + | 636 | WP_000934978 | A24 family peptidase | - |
| C9Z88_RS27115 (C9Z88_27150) | 45807..46848 | + | 1042 | WP_023155257 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
Host bacterium
| ID | 2800 | GenBank | NZ_RRHD01000154 |
| Plasmid name | FWSEC0138|unnamed3 | Incompatibility group | IncI2 |
| Plasmid size | 46848 bp | Coordinate of oriT [Strand] | 3618..3670 [+] |
| Host baterium | Escherichia coli strain FWSEC0138 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |