Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102345 |
| Name | oriT_22|unnamed1  |
| Organism | Klebsiella pneumoniae strain 22 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_PNXO01000019 (81124..81172 [+], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
| Location of nic site | 32..33 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_22|unnamed1
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1780 | GenBank | WP_014386206 |
| Name | traI_C1H63_RS26120_22|unnamed1 |
UniProt ID | G7RVC9 |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190526.05 Da Isoelectric Point: 5.9990
>WP_014386206.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MLSFSQVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTQKQEKEIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTQKQEKEIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 1363 | GenBank | WP_242461604 |
| Name | traD_C1H63_RS26125_22|unnamed1 |
UniProt ID | _ |
| Length | 769 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 769 a.a. Molecular weight: 85929.90 Da Isoelectric Point: 5.1110
>WP_242461604.1 type IV conjugative transfer system coupling protein TraD [Klebsiella pneumoniae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIS
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPASGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAVAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIS
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPASGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAVAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 52184..81730
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C1H63_RS26125 | 52184..54493 | - | 2310 | WP_242461604 | type IV conjugative transfer system coupling protein TraD | virb4 |
| C1H63_RS26130 | 54622..55311 | - | 690 | WP_072101983 | hypothetical protein | - |
| C1H63_RS26135 | 55504..56235 | - | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| C1H63_RS26140 | 56421..56954 | - | 534 | WP_014343486 | conjugal transfer protein TraS | - |
| C1H63_RS26145 | 56957..59806 | - | 2850 | WP_013609534 | conjugal transfer mating-pair stabilization protein TraG | traG |
| C1H63_RS26150 | 59806..61185 | - | 1380 | WP_014343487 | conjugal transfer pilus assembly protein TraH | traH |
| C1H63_RS26155 | 61163..61606 | - | 444 | WP_013214032 | F-type conjugal transfer protein TrbF | - |
| C1H63_RS26160 | 61652..62209 | - | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| C1H63_RS26165 | 62181..62420 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| C1H63_RS26170 | 62431..63183 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| C1H63_RS26175 | 63204..63530 | - | 327 | WP_004152676 | hypothetical protein | - |
| C1H63_RS26180 | 63543..63791 | - | 249 | WP_004152675 | hypothetical protein | - |
| C1H63_RS26185 | 63769..64023 | - | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| C1H63_RS26190 | 64055..66010 | - | 1956 | WP_014343489 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| C1H63_RS26195 | 66069..66707 | - | 639 | WP_004193871 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| C1H63_RS26200 | 66720..67709 | - | 990 | WP_013214027 | conjugal transfer pilus assembly protein TraU | traU |
| C1H63_RS26205 | 67706..68107 | - | 402 | WP_004194979 | hypothetical protein | - |
| C1H63_RS26210 | 68142..68777 | - | 636 | WP_013214026 | type-F conjugative transfer system protein TraW | traW |
| C1H63_RS26215 | 68777..69166 | - | 390 | WP_013214025 | type-F conjugative transfer system protein TrbI | - |
| C1H63_RS26220 | 69166..71805 | - | 2640 | WP_013214024 | type IV secretion system protein TraC | virb4 |
| C1H63_RS26225 | 71877..72275 | - | 399 | WP_023179972 | hypothetical protein | - |
| C1H63_RS26235 | 72654..73058 | - | 405 | WP_004197817 | hypothetical protein | - |
| C1H63_RS26240 | 73125..73436 | - | 312 | WP_094965623 | hypothetical protein | - |
| C1H63_RS26245 | 73437..73655 | - | 219 | WP_014386199 | hypothetical protein | - |
| C1H63_RS28665 | 73679..73969 | - | 291 | WP_014386198 | hypothetical protein | - |
| C1H63_RS26250 | 73974..74384 | - | 411 | WP_014386197 | hypothetical protein | - |
| C1H63_RS26255 | 74515..75099 | - | 585 | WP_014386196 | type IV conjugative transfer system lipoprotein TraV | traV |
| C1H63_RS26260 | 75146..75718 | - | 573 | WP_014386195 | conjugal transfer pilus-stabilizing protein TraP | - |
| C1H63_RS26265 | 75711..77135 | - | 1425 | WP_014386194 | F-type conjugal transfer pilus assembly protein TraB | traB |
| C1H63_RS26270 | 77135..77875 | - | 741 | WP_014386193 | type-F conjugative transfer system secretin TraK | traK |
| C1H63_RS26275 | 77862..78428 | - | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| C1H63_RS26280 | 78448..78753 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| C1H63_RS26285 | 78767..79135 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| C1H63_RS28990 | 79204..79404 | - | 201 | WP_004194116 | TraY domain-containing protein | - |
| C1H63_RS26295 | 79490..80191 | - | 702 | WP_004194113 | hypothetical protein | - |
| C1H63_RS26300 | 80421..80813 | - | 393 | WP_004194114 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| C1H63_RS26305 | 81245..81730 | + | 486 | WP_032440470 | transglycosylase SLT domain-containing protein | virB1 |
| C1H63_RS26310 | 81763..82092 | - | 330 | WP_071994324 | DUF5983 family protein | - |
| C1H63_RS26315 | 82125..82946 | - | 822 | WP_023287138 | DUF932 domain-containing protein | - |
Host bacterium
| ID | 2789 | GenBank | NZ_PNXO01000019 |
| Plasmid name | 22|unnamed1 | Incompatibility group | - |
| Plasmid size | 83145 bp | Coordinate of oriT [Strand] | 81124..81172 [+] |
| Host baterium | Klebsiella pneumoniae strain 22 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |