Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102342
Name   oriT_L01C.1|unnamed2 in_silico
Organism   Legionella pneumophila subsp. pneumophila strain L01C.1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_QWDR01000006 (40537..40569 [+], 33 nt)
oriT length   33 nt
IRs (inverted repeats)      3..9, 13..19  (ACGTTGC..GCAACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 33 nt

>oriT_L01C.1|unnamed2
GCACGTTGCAAAGCAACGTATAAGCGCGCACTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1778 GenBank   WP_120130605
Name   traA_D1H98_RS16820_L01C.1|unnamed2 insolico UniProt ID   _
Length   889 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101200.09 Da        Isoelectric Point: 6.7988

>WP_120130605.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Legionella]
MAIYRFSAQPISRSQGRSVVASSAYRSGEKIIDERSGAIYDYTDKNDVVYKEILLPESAPQWMSERSKLW
NAVELGENRKDAQLSREVQLALPRELTIEQNTELVREFVKTEFVARGMVADVCFHNPKGDDGFHQPHAHI
LLTLREVNEEGFGLKERAWNDKGLLEHWREEWANSQNRHLALHGIDQRVDHRSYKEQGIPLEPQYKIGAK
CAQQHMARLADHQRIAKENGQLIFEKPEIALDALTRNQSTFTHQDLARFINRHTEDAEQFKLVFERVKSS
AELVFVGLDSEGKQRFSTHDMLAIESAMMKQADKLYHQKGHQVDEHAVRNAKSNRTLSQEQETALDYVTS
IGDMKSLLGYAGTGKSYLLGAAREAWEQSGYRVQGTALSGIAALNLSDSSGIESRTIASLFYRLDKGMLT
LNSRDILVVDEAGMLGSRTMERLTFEVEKASAKLVLVGDWQQLQAIEAGAAFRSIAENYQYAELTQIRRQ
TTLWQVEASLHLAQGQVEHALHAYDIHDHIHRFKTTSEAKQSLIEQWNDVRIAQPSESQIILAYTRKDVA
ELNDMARRIKRGDGELGEDIVFSMERGERAFAVNDRVYFLKREDSLSVINGTLGTIQGIDAKSGVITVAL
DRDDLNKKPQLVQVNTNYYKHMEHGYAATVYKAQGVTVDRAYVLPTNHYDAHSTYVAMTRHRKSCDVFVS
REVFAHDRALVYALNRNRAKDVTLDYTQMGREFARQRGLISESMPLPSLSKDEHFRKSEPPLSSLMNQVM
GRDVELEQQKLDAFERQFKKDNPNEALHIANSLLSNAEYGAKSLAGLFAPFSQAIARNQLTSTQNDELIR
LMGKLPLDSQVMNAFKKDYPEFSKQAEQFIKRNEQQLMRIKTIDKELDL

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-213)

AAA domain (AAA_30)

(336-523)


  Protein structure



No available structure.




T4CP

ID   1361 GenBank   WP_120130603
Name   traD_D1H98_RS16810_L01C.1|unnamed2 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 71139.51 Da        Isoelectric Point: 7.4039

>WP_120130603.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Legionella]
MSKDPNFKHYTRGGQISFHNLRMWDQITKTLSMICFFLWIVFTVIFAWIFIPLEKITHVIVFYYAQFLSL
VGQKHHFELSFHGKAYTQTVDSILHYSYYQNNATHVINSLGKYAFWAFCLSFILGLGLAYYFIKRGKAQS
DSQFVRGSQLKNSHEVKKKILRDKSDSDIKIDQFPLIKDSEVQHLLVHGTVGTGKSQLIMKIMDCLRKRG
DRVIVYDKGCSFIPHYYQDESDVILNPFDKRCANWDMWLEAPRDSDFENMAESSIPMHGESDPFWVNASR
TVFSCLGSVMRHHSDRSLDKFLKLILTDEFSALEEYLQGTPAATLVSGKIEKTAISIRATLTTYLKSFSA
LAELHQEGKQPFSIRDYILDEQQKGWLFISSNGETHKSLKPLISMWLAQASLALLSLTPNRNRRIWFICD
ELPSLHKLPLLGETIAEVRKFGGCFLLGMQSFSQLTKVYGQAGAKEMFDLLNTRFFFRSPSSDMARLVAS
ELGEEEIEESRENYSYGANSIRDGISLGAQRVTRPIVSYPQIMELQDLHCFVRLPGHYPITQLALDLGSR
TTKNNGFIERSMPTNFNYSQLTSFDDNLESEANGSMGNTPLRINKINEKLQCIEELNLEPL

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(35-126)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(171-555)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 19128..37087

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1H98_RS16695 (D1H98_16700) 14518..15051 + 534 WP_120130580 hypothetical protein -
D1H98_RS16700 (D1H98_16705) 15020..15787 - 768 WP_120130581 VUT family protein -
D1H98_RS16705 (D1H98_16710) 15771..16430 - 660 WP_120130582 S24 family peptidase -
D1H98_RS16710 (D1H98_16715) 16629..16871 + 243 WP_120130583 TraY domain-containing protein -
D1H98_RS16715 (D1H98_16720) 16849..17928 + 1080 WP_120130584 replication protein -
D1H98_RS16720 (D1H98_16725) 17962..18705 + 744 WP_120130585 complement resistance protein TraT -
D1H98_RS16725 (D1H98_16730) 18827..19114 + 288 WP_120130586 hypothetical protein -
D1H98_RS16730 (D1H98_16735) 19128..19424 + 297 WP_120130587 type IV conjugative transfer system protein TraL traL
D1H98_RS16735 (D1H98_16740) 19434..20003 + 570 WP_120130588 type IV conjugative transfer system protein TraE traE
D1H98_RS16740 (D1H98_16745) 19996..20712 + 717 WP_120130589 type-F conjugative transfer system secretin TraK traK
D1H98_RS16745 (D1H98_16750) 20705..22078 + 1374 WP_120130590 TrbI/VirB10 family protein traB
D1H98_RS16750 (D1H98_16755) 22075..22365 + 291 WP_120130591 TraV family lipoprotein traV
D1H98_RS16755 (D1H98_16760) 22356..24944 + 2589 WP_120130592 type IV secretion system protein TraC virb4
D1H98_RS16760 (D1H98_16765) 24941..25279 + 339 WP_241030152 type-F conjugative transfer system protein TrbI -
D1H98_RS16765 (D1H98_16770) 25285..25911 + 627 WP_241030155 type-F conjugative transfer system protein TraW traW
D1H98_RS16770 (D1H98_16775) 25901..26884 + 984 WP_120130595 conjugal transfer pilus assembly protein TraU traU
D1H98_RS16775 (D1H98_16780) 26898..27314 + 417 WP_120130596 type-F conjugative transfer system pilin assembly protein TrbC trbC
D1H98_RS16780 (D1H98_16785) 27311..29017 + 1707 WP_120130597 type-F conjugative transfer system mating-pair stabilization protein TraN traN
D1H98_RS16785 (D1H98_16790) 29010..29753 + 744 WP_120130598 type-F conjugative transfer system pilin assembly protein TraF traF
D1H98_RS16790 (D1H98_16795) 29750..30262 + 513 WP_241030153 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
D1H98_RS16795 (D1H98_16800) 30259..31623 + 1365 WP_120130600 conjugal transfer protein TraH traH
D1H98_RS16800 (D1H98_16805) 31636..34467 + 2832 WP_120130601 conjugal transfer mating-pair stabilization protein TraG traG
D1H98_RS16805 (D1H98_16810) 34467..35222 + 756 WP_241030154 hypothetical protein -
D1H98_RS16810 (D1H98_16815) 35222..37087 + 1866 WP_120130603 type IV conjugative transfer system coupling protein TraD virb4
D1H98_RS16815 (D1H98_16820) 37171..37788 - 618 WP_120130604 hypothetical protein -
D1H98_RS16820 (D1H98_16825) 37799..40468 - 2670 WP_120130605 Ti-type conjugative transfer relaxase TraA -
D1H98_RS16825 (D1H98_16830) 40695..40991 + 297 WP_120130607 mobilization protein -
D1H98_RS17035 41007..41264 + 258 WP_133305313 hypothetical protein -
D1H98_RS16830 (D1H98_16835) 41333..41512 + 180 WP_242450266 hypothetical protein -

Host bacterium

ID   2786 GenBank   NZ_QWDR01000006
Plasmid name   L01C.1|unnamed2 Incompatibility group   -
Plasmid size   51330 bp Coordinate of oriT [Strand]   40537..40569 [+]
Host baterium   Legionella pneumophila subsp. pneumophila strain L01C.1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1