Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102334
Name   oriT_L03B.1|unnamed2 in_silico
Organism   Legionella pneumophila subsp. pneumophila strain L03B.1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_QWDT01000006 (40537..40569 [+], 33 nt)
oriT length   33 nt
IRs (inverted repeats)      3..9, 13..19  (ACGTTGC..GCAACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 33 nt

>oriT_L03B.1|unnamed2
GCACGTTGCAAAGCAACGTATAAGCGCGCACTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1774 GenBank   WP_120130605
Name   traA_D1I00_RS16850_L03B.1|unnamed2 insolico UniProt ID   _
Length   889 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101200.09 Da        Isoelectric Point: 6.7988

>WP_120130605.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Legionella]
MAIYRFSAQPISRSQGRSVVASSAYRSGEKIIDERSGAIYDYTDKNDVVYKEILLPESAPQWMSERSKLW
NAVELGENRKDAQLSREVQLALPRELTIEQNTELVREFVKTEFVARGMVADVCFHNPKGDDGFHQPHAHI
LLTLREVNEEGFGLKERAWNDKGLLEHWREEWANSQNRHLALHGIDQRVDHRSYKEQGIPLEPQYKIGAK
CAQQHMARLADHQRIAKENGQLIFEKPEIALDALTRNQSTFTHQDLARFINRHTEDAEQFKLVFERVKSS
AELVFVGLDSEGKQRFSTHDMLAIESAMMKQADKLYHQKGHQVDEHAVRNAKSNRTLSQEQETALDYVTS
IGDMKSLLGYAGTGKSYLLGAAREAWEQSGYRVQGTALSGIAALNLSDSSGIESRTIASLFYRLDKGMLT
LNSRDILVVDEAGMLGSRTMERLTFEVEKASAKLVLVGDWQQLQAIEAGAAFRSIAENYQYAELTQIRRQ
TTLWQVEASLHLAQGQVEHALHAYDIHDHIHRFKTTSEAKQSLIEQWNDVRIAQPSESQIILAYTRKDVA
ELNDMARRIKRGDGELGEDIVFSMERGERAFAVNDRVYFLKREDSLSVINGTLGTIQGIDAKSGVITVAL
DRDDLNKKPQLVQVNTNYYKHMEHGYAATVYKAQGVTVDRAYVLPTNHYDAHSTYVAMTRHRKSCDVFVS
REVFAHDRALVYALNRNRAKDVTLDYTQMGREFARQRGLISESMPLPSLSKDEHFRKSEPPLSSLMNQVM
GRDVELEQQKLDAFERQFKKDNPNEALHIANSLLSNAEYGAKSLAGLFAPFSQAIARNQLTSTQNDELIR
LMGKLPLDSQVMNAFKKDYPEFSKQAEQFIKRNEQQLMRIKTIDKELDL

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-213)

AAA domain (AAA_30)

(336-523)


  Protein structure



No available structure.




T4CP

ID   1358 GenBank   WP_120130603
Name   traD_D1I00_RS16840_L03B.1|unnamed2 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 71139.51 Da        Isoelectric Point: 7.4039

>WP_120130603.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Legionella]
MSKDPNFKHYTRGGQISFHNLRMWDQITKTLSMICFFLWIVFTVIFAWIFIPLEKITHVIVFYYAQFLSL
VGQKHHFELSFHGKAYTQTVDSILHYSYYQNNATHVINSLGKYAFWAFCLSFILGLGLAYYFIKRGKAQS
DSQFVRGSQLKNSHEVKKKILRDKSDSDIKIDQFPLIKDSEVQHLLVHGTVGTGKSQLIMKIMDCLRKRG
DRVIVYDKGCSFIPHYYQDESDVILNPFDKRCANWDMWLEAPRDSDFENMAESSIPMHGESDPFWVNASR
TVFSCLGSVMRHHSDRSLDKFLKLILTDEFSALEEYLQGTPAATLVSGKIEKTAISIRATLTTYLKSFSA
LAELHQEGKQPFSIRDYILDEQQKGWLFISSNGETHKSLKPLISMWLAQASLALLSLTPNRNRRIWFICD
ELPSLHKLPLLGETIAEVRKFGGCFLLGMQSFSQLTKVYGQAGAKEMFDLLNTRFFFRSPSSDMARLVAS
ELGEEEIEESRENYSYGANSIRDGISLGAQRVTRPIVSYPQIMELQDLHCFVRLPGHYPITQLALDLGSR
TTKNNGFIERSMPTNFNYSQLTSFDDNLESEANGSMGNTPLRINKINEKLQCIEELNLEPL

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(35-126)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(171-555)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 19128..37087

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1I00_RS16725 (D1I00_16720) 14518..15051 + 534 WP_120130580 hypothetical protein -
D1I00_RS16730 (D1I00_16725) 15020..15787 - 768 WP_120130581 VUT family protein -
D1I00_RS16735 (D1I00_16730) 15771..16430 - 660 WP_120130582 S24 family peptidase -
D1I00_RS16740 (D1I00_16735) 16629..16871 + 243 WP_120130583 TraY domain-containing protein -
D1I00_RS16745 (D1I00_16740) 16849..17928 + 1080 WP_120130584 replication protein -
D1I00_RS16750 (D1I00_16745) 17962..18705 + 744 WP_120130585 complement resistance protein TraT -
D1I00_RS16755 (D1I00_16750) 18827..19114 + 288 WP_120130586 hypothetical protein -
D1I00_RS16760 (D1I00_16755) 19128..19424 + 297 WP_120130587 type IV conjugative transfer system protein TraL traL
D1I00_RS16765 (D1I00_16760) 19434..20003 + 570 WP_120130588 type IV conjugative transfer system protein TraE traE
D1I00_RS16770 (D1I00_16765) 19996..20712 + 717 WP_120130589 type-F conjugative transfer system secretin TraK traK
D1I00_RS16775 (D1I00_16770) 20705..22078 + 1374 WP_120130590 TrbI/VirB10 family protein traB
D1I00_RS16780 (D1I00_16775) 22075..22365 + 291 WP_120130591 TraV family lipoprotein traV
D1I00_RS16785 (D1I00_16780) 22356..24944 + 2589 WP_120130592 type IV secretion system protein TraC virb4
D1I00_RS16790 (D1I00_16785) 24941..25279 + 339 WP_241030152 type-F conjugative transfer system protein TrbI -
D1I00_RS16795 (D1I00_16790) 25285..25911 + 627 WP_241030155 type-F conjugative transfer system protein TraW traW
D1I00_RS16800 (D1I00_16795) 25901..26884 + 984 WP_120130595 conjugal transfer pilus assembly protein TraU traU
D1I00_RS16805 (D1I00_16800) 26898..27314 + 417 WP_120130596 type-F conjugative transfer system pilin assembly protein TrbC trbC
D1I00_RS16810 (D1I00_16805) 27311..29017 + 1707 WP_120130597 type-F conjugative transfer system mating-pair stabilization protein TraN traN
D1I00_RS16815 (D1I00_16810) 29010..29753 + 744 WP_120130598 type-F conjugative transfer system pilin assembly protein TraF traF
D1I00_RS16820 (D1I00_16815) 29750..30262 + 513 WP_241030153 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
D1I00_RS16825 (D1I00_16820) 30259..31623 + 1365 WP_120130600 conjugal transfer protein TraH traH
D1I00_RS16830 (D1I00_16825) 31636..34467 + 2832 WP_120130601 conjugal transfer mating-pair stabilization protein TraG traG
D1I00_RS16835 (D1I00_16830) 34467..35222 + 756 WP_241030154 hypothetical protein -
D1I00_RS16840 (D1I00_16835) 35222..37087 + 1866 WP_120130603 type IV conjugative transfer system coupling protein TraD virb4
D1I00_RS16845 (D1I00_16840) 37171..37788 - 618 WP_120130604 hypothetical protein -
D1I00_RS16850 (D1I00_16845) 37799..40468 - 2670 WP_120130605 Ti-type conjugative transfer relaxase TraA -
D1I00_RS16860 (D1I00_16855) 40695..40991 + 297 WP_120130607 mobilization protein -
D1I00_RS17065 41007..41264 + 258 WP_133305313 hypothetical protein -
D1I00_RS16865 (D1I00_16860) 41333..41512 + 180 WP_242450266 hypothetical protein -

Host bacterium

ID   2778 GenBank   NZ_QWDT01000006
Plasmid name   L03B.1|unnamed2 Incompatibility group   -
Plasmid size   51330 bp Coordinate of oriT [Strand]   40537..40569 [+]
Host baterium   Legionella pneumophila subsp. pneumophila strain L03B.1

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1