Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102329 |
| Name | oriT_pS1  |
| Organism | Dickeya fangzhongdai strain S1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JXBO02000024 (853..963 [-], 111 nt) |
| oriT length | 111 nt |
| IRs (inverted repeats) | 71..78, 81..88 (CACCATGC..GCATGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 111 nt
>oriT_pS1
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1771 | GenBank | WP_127350806 |
| Name | Relaxase_QR69_RS19735_pS1 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 609 a.a. Molecular weight: 66802.56 Da Isoelectric Point: 10.5441
>WP_127350806.1 TraI/MobA(P) family conjugative relaxase [Dickeya fangzhongdai]
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICDGLGLGEHQRVSAVHNDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCTTLERDYGLERDNHEPRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKDAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQAAPARRNYQKGRPMRLRGGLD
TTELYARYKGAQQTLTAARAGVLAEAKRWKDRAIEHAKKAAATRRAAIKHLTDGRLTKKLLYAQASAALR
ADLEAIHKRHDQERAAIYNEHRRRPWADWLKQQATAGDGQALDALRAREAAQGLKGDTIQGDGQAKPGHA
PVIDSITKKGTIIYRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRPGVPPVGQEPPPHRRHGLRTLGQLDGLHIEGEAQRPVQAP
TQATPTPPPAKVQQPARPAPSELEQRETRLRAQMEAKKAKRQEGKKRGR
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICDGLGLGEHQRVSAVHNDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCTTLERDYGLERDNHEPRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKDAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQAAPARRNYQKGRPMRLRGGLD
TTELYARYKGAQQTLTAARAGVLAEAKRWKDRAIEHAKKAAATRRAAIKHLTDGRLTKKLLYAQASAALR
ADLEAIHKRHDQERAAIYNEHRRRPWADWLKQQATAGDGQALDALRAREAAQGLKGDTIQGDGQAKPGHA
PVIDSITKKGTIIYRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRPGVPPVGQEPPPHRRHGLRTLGQLDGLHIEGEAQRPVQAP
TQATPTPPPAKVQQPARPAPSELEQRETRLRAQMEAKKAKRQEGKKRGR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 698 | GenBank | WP_054471493 |
| Name | WP_054471493_pS1 |
UniProt ID | A0A120LKZ5 |
| Length | 125 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 125 a.a. Molecular weight: 13520.32 Da Isoelectric Point: 7.9653
>WP_054471493.1 MULTISPECIES: conjugal transfer transcriptional regulator TraJ [Pseudomonadota]
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGQS
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGQS
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A120LKZ5 |
Host bacterium
| ID | 2773 | GenBank | NZ_JXBO02000024 |
| Plasmid name | pS1 | Incompatibility group | - |
| Plasmid size | 23083 bp | Coordinate of oriT [Strand] | 853..963 [-] |
| Host baterium | Dickeya fangzhongdai strain S1 |
Cargo genes
| Drug resistance gene | aph(3'')-Ib, aph(6)-Id |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |