Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102326
Name   oriT_p7209-49 in_silico
Organism   Serratia marcescens strain 7209
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_PEHC01000009 (1481..1586 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_p7209-49
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1770 GenBank   WP_101453311
Name   Relaxase_CS371_RS14350_p7209-49 insolico UniProt ID   _
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75193.92 Da        Isoelectric Point: 9.9948

>WP_101453311.1 TraI/MobA(P) family conjugative relaxase [Serratia marcescens]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAALNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure



No available structure.




T4CP

ID   1355 GenBank   WP_101453316
Name   t4cp2_CS371_RS14465_p7209-49 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79539.85 Da        Isoelectric Point: 4.7667

>WP_101453316.1 conjugal transfer protein TrbC [Serratia marcescens]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYERARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 4382..31681

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS371_RS14335 (CS371_14190) 1..446 + 446 WP_101453310 hypothetical protein -
CS371_RS14340 (CS371_14195) 948..1343 - 396 WP_019725163 hypothetical protein -
CS371_RS28675 1378..1905 + 528 WP_172740549 plasmid mobilization protein MobA -
CS371_RS14350 (CS371_14205) 1892..3871 + 1980 WP_101453311 TraI/MobA(P) family conjugative relaxase -
CS371_RS14355 (CS371_14210) 3885..4385 + 501 WP_004187320 DotD/TraH family lipoprotein -
CS371_RS14360 (CS371_14215) 4382..5161 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
CS371_RS14365 (CS371_14220) 5172..6335 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
CS371_RS14370 (CS371_14225) 6325..6585 + 261 WP_004187310 IcmT/TraK family protein traK
CS371_RS28680 6610..9813 + 3204 WP_242490474 LPD7 domain-containing protein -
CS371_RS27975 (CS371_14235) 9968..10480 + 513 WP_011091071 hypothetical protein traL
CS371_RS14380 (CS371_14240) 10481..10693 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
CS371_RS14385 (CS371_14245) 10665..11075 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
CS371_RS14390 (CS371_14250) 11143..11856 + 714 WP_004187467 DotI/IcmL family type IV secretion protein traM
CS371_RS14400 (CS371_14255) 11865..13016 + 1152 WP_004187471 DotH/IcmK family type IV secretion protein traN
CS371_RS28685 13028..13225 + 198 WP_242490475 hypothetical protein traO
CS371_RS14405 (CS371_14260) 13229..14377 + 1149 WP_277949772 conjugal transfer protein TraO traO
CS371_RS14410 (CS371_14265) 14389..15093 + 705 WP_015060002 conjugal transfer protein TraP traP
CS371_RS14415 (CS371_14270) 15117..15647 + 531 WP_004187478 conjugal transfer protein TraQ traQ
CS371_RS14420 (CS371_14275) 15664..16053 + 390 WP_004187479 DUF6750 family protein traR
CS371_RS14425 (CS371_14280) 16099..16593 + 495 WP_004187480 hypothetical protein -
CS371_RS14430 (CS371_14285) 16590..19640 + 3051 WP_101453313 conjugal transfer protein traU
CS371_RS14435 (CS371_14290) 19637..20845 + 1209 WP_101453314 conjugal transfer protein TraW traW
CS371_RS14440 (CS371_14295) 20842..21438 + 597 WP_015060003 hypothetical protein -
CS371_RS28960 21431..22735 + 1305 WP_277949771 DotA/TraY family protein traY
CS371_RS28965 22722..23627 + 906 WP_277949773 DotA/TraY family protein traY
CS371_RS14450 (CS371_14305) 23629..24282 + 654 WP_015060005 hypothetical protein -
CS371_RS14455 (CS371_14310) 24351..24593 + 243 WP_023893611 IncL/M type plasmid replication protein RepC -
CS371_RS14460 (CS371_14315) 24889..25944 + 1056 WP_015060006 plasmid replication initiator RepA -
CS371_RS28970 27167..27262 + 96 WP_004206884 DinQ-like type I toxin DqlB -
CS371_RS14465 (CS371_14320) 27313..29400 - 2088 WP_101453316 conjugal transfer protein TrbC -
CS371_RS14470 (CS371_14325) 29413..30363 - 951 WP_004206886 DsbC family protein trbB
CS371_RS14475 (CS371_14330) 30374..31681 - 1308 WP_242490476 conjugal transfer protein TrbA trbA
CS371_RS14480 (CS371_14335) 31681..32076 - 396 WP_004187436 lytic transglycosylase domain-containing protein -
CS371_RS14485 (CS371_14340) 32181..32564 - 384 WP_019725042 DUF1496 domain-containing protein -
CS371_RS14490 (CS371_14345) 32644..33297 + 654 WP_004206890 type II CAAX endopeptidase family protein -
CS371_RS27980 33389..33646 + 258 WP_004187429 type II toxin-antitoxin system antitoxin PemI -
CS371_RS14495 (CS371_14350) 33648..33980 + 333 WP_144236188 type II toxin-antitoxin system toxin endoribonuclease PemK-mt -
CS371_RS14500 (CS371_14355) 34073..34507 + 435 WP_004187425 translesion error-prone DNA polymerase V autoproteolytic subunit -
CS371_RS14505 (CS371_14360) 34495..35762 + 1268 Protein_38 translesion error-prone DNA polymerase V subunit UmuC -
CS371_RS14510 (CS371_14365) 35885..36094 - 210 WP_004187413 hypothetical protein -
CS371_RS28690 36230..36652 - 423 WP_242490477 hypothetical protein -

Host bacterium

ID   2770 GenBank   NZ_PEHC01000009
Plasmid name   p7209-49 Incompatibility group   IncL/M
Plasmid size   48903 bp Coordinate of oriT [Strand]   1481..1586 [+]
Host baterium   Serratia marcescens strain 7209

Cargo genes

Drug resistance gene   blaTEM-1C, blaCTX-M-15
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -