Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102320
Name   oriT_KPC89|unnamed P38 in_silico
Organism   Klebsiella pneumoniae strain KPC89
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_QXGQ01000060 (49137..49186 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_KPC89|unnamed P38
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1768 GenBank   WP_004153029
Name   traI_DWC17_RS02630_KPC89|unnamed P38 insolico UniProt ID   A0A422WEA5
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190808.90 Da        Isoelectric Point: 6.2081

>WP_004153029.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1433-1555)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

AAA domain (AAA_30)

(971-1157)

TrwC relaxase (TrwC)

(10-284)


  Protein structure



No available structure.




T4CP

ID   1352 GenBank   WP_004152630
Name   traD_DWC17_RS02635_KPC89|unnamed P38 insolico UniProt ID   _
Length   769 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 769 a.a.        Molecular weight: 85951.02 Da        Isoelectric Point: 5.0632

>WP_004152630.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 21326..49744

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DWC17_RS02635 (DWC17_02645) 21326..23635 - 2310 WP_004152630 type IV conjugative transfer system coupling protein TraD virb4
DWC17_RS02640 (DWC17_02650) 23995..24726 - 732 WP_004152629 conjugal transfer complement resistance protein TraT -
DWC17_RS02645 (DWC17_02655) 24916..25443 - 528 WP_004153030 hypothetical protein -
DWC17_RS02650 (DWC17_02660) 25449..28298 - 2850 WP_004178054 conjugal transfer mating-pair stabilization protein TraG traG
DWC17_RS02655 (DWC17_02665) 28298..29668 - 1371 WP_004178055 conjugal transfer pilus assembly protein TraH traH
DWC17_RS02660 (DWC17_02670) 29655..30083 - 429 WP_004152689 hypothetical protein -
DWC17_RS02665 (DWC17_02675) 30076..30648 - 573 WP_004152688 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
DWC17_RS02670 (DWC17_02680) 30620..30859 - 240 WP_004152687 type-F conjugative transfer system pilin chaperone TraQ -
DWC17_RS02675 (DWC17_02685) 30870..31622 - 753 WP_004152686 type-F conjugative transfer system pilin assembly protein TraF traF
DWC17_RS02680 (DWC17_02690) 31643..31969 - 327 WP_004152685 hypothetical protein -
DWC17_RS30545 (DWC17_02695) 32015..32200 - 186 WP_004152684 hypothetical protein -
DWC17_RS02690 (DWC17_02700) 32197..32433 - 237 WP_004152683 conjugal transfer protein TrbE -
DWC17_RS02695 (DWC17_02705) 32465..34420 - 1956 WP_004153093 type-F conjugative transfer system mating-pair stabilization protein TraN traN
DWC17_RS02700 (DWC17_02710) 34468..35106 - 639 WP_004152682 type-F conjugative transfer system pilin assembly protein TrbC trbC
DWC17_RS02705 (DWC17_02715) 35119..36078 - 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
DWC17_RS02710 (DWC17_02720) 36122..36748 - 627 WP_004152590 type-F conjugative transfer system protein TraW traW
DWC17_RS02715 (DWC17_02725) 36748..37137 - 390 WP_004152591 type-F conjugative transfer system protein TrbI -
DWC17_RS02720 (DWC17_02730) 37137..39776 - 2640 WP_004152592 type IV secretion system protein TraC virb4
DWC17_RS02725 (DWC17_02735) 39848..40246 - 399 WP_004153071 hypothetical protein -
DWC17_RS02735 (DWC17_02745) 40542..40946 - 405 WP_004152594 hypothetical protein -
DWC17_RS02740 (DWC17_02750) 41013..41330 - 318 WP_004152595 hypothetical protein -
DWC17_RS02745 (DWC17_02755) 41331..41549 - 219 WP_004171484 hypothetical protein -
DWC17_RS02750 (DWC17_02760) 41573..41863 - 291 WP_004152596 hypothetical protein -
DWC17_RS02755 (DWC17_02765) 41868..42278 - 411 WP_004152597 lipase chaperone -
DWC17_RS02760 (DWC17_02770) 42410..42979 - 570 WP_004152598 type IV conjugative transfer system lipoprotein TraV traV
DWC17_RS02765 (DWC17_02775) 43002..43598 - 597 WP_004152599 conjugal transfer pilus-stabilizing protein TraP -
DWC17_RS02770 (DWC17_02780) 43591..45015 - 1425 WP_004152600 F-type conjugal transfer pilus assembly protein TraB traB
DWC17_RS02775 (DWC17_02785) 45015..45755 - 741 WP_004152601 type-F conjugative transfer system secretin TraK traK
DWC17_RS02780 (DWC17_02790) 45742..46308 - 567 WP_004152602 type IV conjugative transfer system protein TraE traE
DWC17_RS02785 (DWC17_02795) 46328..46633 - 306 WP_004178059 type IV conjugative transfer system protein TraL traL
DWC17_RS02790 (DWC17_02800) 46647..47015 - 369 WP_004178060 type IV conjugative transfer system pilin TraA -
DWC17_RS02795 (DWC17_02805) 47069..47440 - 372 WP_004208838 TraY domain-containing protein -
DWC17_RS02800 (DWC17_02810) 47524..48219 - 696 WP_004178061 transcriptional regulator TraJ family protein -
DWC17_RS02805 (DWC17_02815) 48433..48825 - 393 WP_004178062 conjugal transfer relaxosome DNA-binding protein TraM -
DWC17_RS02810 (DWC17_02820) 49259..49744 + 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
DWC17_RS02815 (DWC17_02825) 49777..50106 - 330 WP_011977736 DUF5983 family protein -
DWC17_RS02820 (DWC17_02830) 50139..50960 - 822 WP_004178064 DUF932 domain-containing protein -

Host bacterium

ID   2764 GenBank   NZ_QXGQ01000060
Plasmid name   KPC89|unnamed P38 Incompatibility group   IncFII
Plasmid size   50992 bp Coordinate of oriT [Strand]   49137..49186 [+]
Host baterium   Klebsiella pneumoniae strain KPC89

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -