Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102312
Name   oriT_B29|unnamed P53 in_silico
Organism   Klebsiella pneumoniae strain B29
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_NTHW02000076 (50434..50483 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_B29|unnamed P53
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1766 GenBank   WP_004153029
Name   traI_CK504_RS27485_B29|unnamed P53 insolico UniProt ID   A0A422WEA5
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190808.90 Da        Isoelectric Point: 6.2081

>WP_004153029.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1433-1555)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

AAA domain (AAA_30)

(971-1157)

TrwC relaxase (TrwC)

(10-284)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 26746..51041

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CK504_RS27490 (CK504_027510) 22712..24932 - 2221 Protein_19 type IV secretion system DNA-binding domain-containing protein -
CK504_RS27495 (CK504_027515) 25292..26023 - 732 WP_004152629 conjugal transfer complement resistance protein TraT -
CK504_RS27500 (CK504_027520) 26213..26740 - 528 WP_004153030 hypothetical protein -
CK504_RS27505 (CK504_027525) 26746..29595 - 2850 WP_004178054 conjugal transfer mating-pair stabilization protein TraG traG
CK504_RS27510 (CK504_027530) 29595..30965 - 1371 WP_004178055 conjugal transfer pilus assembly protein TraH traH
CK504_RS27515 (CK504_027535) 30952..31380 - 429 WP_004152689 hypothetical protein -
CK504_RS27520 (CK504_027540) 31373..31945 - 573 WP_004152688 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
CK504_RS27525 (CK504_027545) 31917..32156 - 240 WP_004152687 type-F conjugative transfer system pilin chaperone TraQ -
CK504_RS27530 (CK504_027550) 32167..32919 - 753 WP_004152686 type-F conjugative transfer system pilin assembly protein TraF traF
CK504_RS27535 (CK504_027555) 32940..33266 - 327 WP_004152685 hypothetical protein -
CK504_RS29280 (CK504_027560) 33312..33497 - 186 WP_004152684 hypothetical protein -
CK504_RS27545 (CK504_027565) 33494..33730 - 237 WP_004152683 conjugal transfer protein TrbE -
CK504_RS27550 (CK504_027570) 33762..35717 - 1956 WP_004153093 type-F conjugative transfer system mating-pair stabilization protein TraN traN
CK504_RS27555 (CK504_027575) 35765..36403 - 639 WP_004152682 type-F conjugative transfer system pilin assembly protein TrbC trbC
CK504_RS27560 (CK504_027580) 36416..37375 - 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
CK504_RS27565 (CK504_027585) 37419..38045 - 627 WP_004152590 type-F conjugative transfer system protein TraW traW
CK504_RS27570 (CK504_027590) 38045..38434 - 390 WP_004152591 type-F conjugative transfer system protein TrbI -
CK504_RS27575 (CK504_027595) 38434..41073 - 2640 WP_004152592 type IV secretion system protein TraC virb4
CK504_RS27580 (CK504_027600) 41145..41543 - 399 WP_004153071 hypothetical protein -
CK504_RS27590 (CK504_027610) 41839..42243 - 405 WP_004152594 hypothetical protein -
CK504_RS27595 (CK504_027615) 42310..42627 - 318 WP_004152595 hypothetical protein -
CK504_RS27600 (CK504_027620) 42628..42846 - 219 WP_004171484 hypothetical protein -
CK504_RS27605 (CK504_027625) 42870..43160 - 291 WP_004152596 hypothetical protein -
CK504_RS27610 (CK504_027630) 43165..43575 - 411 WP_004152597 lipase chaperone -
CK504_RS27615 (CK504_027635) 43707..44276 - 570 WP_004152598 type IV conjugative transfer system lipoprotein TraV traV
CK504_RS27620 (CK504_027640) 44299..44895 - 597 WP_004152599 conjugal transfer pilus-stabilizing protein TraP -
CK504_RS27625 (CK504_027645) 44888..46312 - 1425 WP_004152600 F-type conjugal transfer pilus assembly protein TraB traB
CK504_RS27630 (CK504_027650) 46312..47052 - 741 WP_004152601 type-F conjugative transfer system secretin TraK traK
CK504_RS27635 (CK504_027655) 47039..47605 - 567 WP_004152602 type IV conjugative transfer system protein TraE traE
CK504_RS27640 (CK504_027660) 47625..47930 - 306 WP_004178059 type IV conjugative transfer system protein TraL traL
CK504_RS27645 (CK504_027665) 47944..48312 - 369 WP_004178060 type IV conjugative transfer system pilin TraA -
CK504_RS27650 (CK504_027670) 48366..48737 - 372 WP_004208838 TraY domain-containing protein -
CK504_RS27655 (CK504_027675) 48821..49516 - 696 WP_004178061 transcriptional regulator TraJ family protein -
CK504_RS27660 (CK504_027680) 49730..50122 - 393 WP_004178062 conjugal transfer relaxosome DNA-binding protein TraM -
CK504_RS27665 (CK504_027685) 50556..51041 + 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
CK504_RS27670 (CK504_027690) 51074..51403 - 330 WP_011977736 DUF5983 family protein -
CK504_RS27675 (CK504_027695) 51436..52257 - 822 WP_004178064 DUF932 domain-containing protein -
CK504_RS27695 (CK504_027715) 52992..53405 - 414 WP_004152722 helix-turn-helix domain-containing protein -
CK504_RS27700 (CK504_027720) 53406..53684 - 279 WP_004152721 helix-turn-helix transcriptional regulator -
CK504_RS27705 (CK504_027725) 53674..53994 - 321 WP_004152720 type II toxin-antitoxin system RelE/ParE family toxin -
CK504_RS27710 (CK504_027730) 54075..54299 - 225 WP_004152719 hypothetical protein -
CK504_RS27715 (CK504_027735) 54310..54522 - 213 WP_004152718 hypothetical protein -
CK504_RS27720 (CK504_027740) 54583..54939 - 357 WP_004152717 hypothetical protein -
CK504_RS27725 (CK504_027745) 55446..55763 - 318 WP_004118473 hypothetical protein -

Host bacterium

ID   2756 GenBank   NZ_NTHW02000076
Plasmid name   B29|unnamed P53 Incompatibility group   IncFII
Plasmid size   57793 bp Coordinate of oriT [Strand]   50434..50483 [+]
Host baterium   Klebsiella pneumoniae strain B29

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -