Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102311
Name   oriT_B29|unnamed P14a in_silico
Organism   Klebsiella pneumoniae strain B29
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_NTHW02000065 (56073..56161 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_B29|unnamed P14a
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1765 GenBank   WP_052938718
Name   nikB_CK504_RS26885_B29|unnamed P14a insolico UniProt ID   A0A706EBF4
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104042.50 Da        Isoelectric Point: 7.3623

>WP_052938718.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLAERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A706EBF4


Auxiliary protein

ID   697 GenBank   WP_052938717
Name   WP_052938717_B29|unnamed P14a insolico UniProt ID   A0A706B4V0
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12643.59 Da        Isoelectric Point: 10.7463

>WP_052938717.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSTVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A706B4V0


T4CP

ID   1350 GenBank   WP_021497839
Name   trbC_CK504_RS26880_B29|unnamed P14a insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86967.19 Da        Isoelectric Point: 7.0662

>WP_021497839.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLKCADPSQDRMIKRSLFSFWPTLFQYEVILESPANGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12402..50695

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CK504_RS26640 (CK504_026660) 7464..8531 + 1068 WP_054170954 type IV pilus biogenesis lipoprotein PilL -
CK504_RS26645 (CK504_026665) 8531..8968 + 438 WP_000539811 type IV pilus biogenesis protein PilM -
CK504_RS26650 (CK504_026670) 8982..10664 + 1683 WP_054170956 PilN family type IVB pilus formation outer membrane protein -
CK504_RS26655 (CK504_026675) 10657..11952 + 1296 WP_000752780 type 4b pilus protein PilO2 -
CK504_RS26660 (CK504_026680) 11939..12391 + 453 WP_001247337 type IV pilus biogenesis protein PilP -
CK504_RS26665 (CK504_026685) 12402..13955 + 1554 WP_000362206 ATPase, T2SS/T4P/T4SS family virB11
CK504_RS26670 (CK504_026690) 13968..15053 + 1086 WP_054170959 type II secretion system F family protein -
CK504_RS26675 (CK504_026695) 15070..15684 + 615 WP_000908226 type 4 pilus major pilin -
CK504_RS26680 (CK504_026700) 15694..16254 + 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
CK504_RS26685 (CK504_026705) 16239..16895 + 657 WP_001193549 A24 family peptidase -
CK504_RS26690 (CK504_026710) 16883..18319 + 1437 WP_015058206 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
CK504_RS29250 (CK504_026715) 18316..18537 - 222 Protein_19 prepilin -
CK504_RS26730 (CK504_026750) 19953..21107 + 1155 WP_001139955 site-specific integrase -
CK504_RS26735 (CK504_026755) 21258..22082 + 825 WP_001690630 conjugal transfer protein TraE traE
CK504_RS26740 (CK504_026760) 22168..23370 + 1203 WP_000976353 conjugal transfer protein TraF -
CK504_RS26745 (CK504_026765) 23430..24014 + 585 WP_000977525 histidine phosphatase family protein -
CK504_RS26750 (CK504_026770) 24409..24867 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
CK504_RS26755 (CK504_026775) 24864..25682 + 819 WP_032220737 IncI1-type conjugal transfer lipoprotein TraI traI
CK504_RS26760 (CK504_026780) 25679..26827 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
CK504_RS26765 (CK504_026785) 26824..27114 + 291 WP_001299214 hypothetical protein traK
CK504_RS26770 (CK504_026790) 27129..27680 + 552 WP_000014586 phospholipase D family protein -
CK504_RS26775 (CK504_026795) 27770..31534 + 3765 WP_021497833 LPD7 domain-containing protein -
CK504_RS26780 (CK504_026800) 31552..31899 + 348 WP_001055900 conjugal transfer protein traL
CK504_RS26785 (CK504_026805) 31896..32588 + 693 WP_000138551 DotI/IcmL family type IV secretion protein traM
CK504_RS26790 (CK504_026810) 32599..33582 + 984 WP_052938673 IncI1-type conjugal transfer protein TraN traN
CK504_RS26795 (CK504_026815) 33585..34874 + 1290 WP_001271991 conjugal transfer protein TraO traO
CK504_RS26800 (CK504_026820) 34874..35578 + 705 WP_060614393 IncI1-type conjugal transfer protein TraP traP
CK504_RS26805 (CK504_026825) 35578..36105 + 528 WP_001055569 conjugal transfer protein TraQ traQ
CK504_RS26810 (CK504_026830) 36156..36560 + 405 WP_021497835 IncI1-type conjugal transfer protein TraR traR
CK504_RS26815 (CK504_026835) 36624..36812 + 189 WP_001277255 putative conjugal transfer protein TraS -
CK504_RS26820 (CK504_026840) 36796..37596 + 801 WP_021497829 IncI1-type conjugal transfer protein TraT traT
CK504_RS26825 (CK504_026845) 37686..40730 + 3045 WP_001024782 IncI1-type conjugal transfer protein TraU traU
CK504_RS26830 (CK504_026850) 40730..41344 + 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
CK504_RS26835 (CK504_026855) 41311..42513 + 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
CK504_RS26840 (CK504_026860) 42542..43126 + 585 WP_001476649 IncI1-type conjugal transfer protein TraX -
CK504_RS26845 (CK504_026865) 43223..45391 + 2169 WP_054170920 DotA/TraY family protein traY
CK504_RS26850 (CK504_026870) 45465..46115 + 651 WP_001178506 plasmid IncI1-type surface exclusion protein ExcA -
CK504_RS26855 (CK504_026875) 46187..46396 - 210 WP_000062603 HEAT repeat domain-containing protein -
CK504_RS28750 46796..46972 + 177 WP_001054898 hypothetical protein -
CK504_RS29215 47037..47132 - 96 WP_001303310 DinQ-like type I toxin DqlB -
CK504_RS26860 (CK504_026880) 47631..47882 + 252 WP_001291964 hypothetical protein -
CK504_RS26865 (CK504_026885) 47954..48106 - 153 WP_001387489 Hok/Gef family protein -
CK504_RS26870 (CK504_026890) 48398..49606 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
CK504_RS26875 (CK504_026895) 49625..50695 + 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
CK504_RS26880 (CK504_026900) 50688..52979 + 2292 WP_021497839 F-type conjugative transfer protein TrbC -

Host bacterium

ID   2755 GenBank   NZ_NTHW02000065
Plasmid name   B29|unnamed P14a Incompatibility group   IncI1
Plasmid size   75000 bp Coordinate of oriT [Strand]   56073..56161 [+]
Host baterium   Klebsiella pneumoniae strain B29

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -