Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102308
• Name: oriT_TRPF4|unnamed2
• Organism: Staphylococcus warneri strain TRPF4
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_PJLY01000022 (17344..17463 [-], 120 nt)
• oriT length: 120 nt
• IRs (inverted repeats): 52..59, 61..68 (TTGGGGAT..ATCCCCAA) 22..29, 34..41 (ATTTTTTT..AAAAAAAT) 24..29, 35..40 (TTTTTT..AAAAAA) 23..28, 34..39 (TTTTTT..AAAAAA) 1..8, 14..21 (AGTGGCTA..TAGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 120 nt

>oriT_TRPF4|unnamed2
AGTGGCTATCAATTAGCCACTATTTTTTTCGCCAAAAAAATCTTAAGGGGCTTGGGGATTATCCCCAACAAGCAGGCGCGTCTGCCACGAGAGTGGCTAGCAAAGCCAATGATTGCCAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_CWE31_RS12840_TRPF4|unnamed2

• ID: 1764
• GenBank: WP_267472202
• Name: Relaxase_CWE31_RS12840_TRPF4|unnamed2
• UniProt ID: _
• Length: 243 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 243 a.a.
• Molecular weight: 27976.34 Da
• Isoelectric Point: 9.2228

>WP_267472202.1 relaxase/mobilization nuclease domain-containing protein [Staphylococcus warneri]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKPDEVTPE
QCNQLGLELAEKIAPNHQVAMYTHNNTDHIHNHIVINAINLETGKKFNNNKQALRDLRDLNDEVCRAHGL
SVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREVIDQSQATNMDEFKDHLNQQCFSLYRVYFRRY
NLVNSRKGALMRTFSSRAKTDIDLRRFFYLNFN

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-203)

Protein structure

No available structure.

Host bacterium

• ID: 2752
• GenBank: NZ_PJLY01000022
• Plasmid name: TRPF4|unnamed2
• Incompatibility group: -
• Plasmid size: 26387 bp
• Coordinate of oriT [Strand]: 17344..17463 [-]
• Host baterium: Staphylococcus warneri strain TRPF4

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21