Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102294
Name   oriT_B15|unnamed P9 in_silico
Organism   Klebsiella pneumoniae strain B15
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_QWDG01000009 (39325..39406 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_B15|unnamed P9
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1758 GenBank   WP_032440569
Name   Relaxase_D1A27_RS01195_B15|unnamed P9 insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   1347 GenBank   WP_032440593
Name   trbC_D1A27_RS01060_B15|unnamed P9 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 8425..30216

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1A27_RS01050 (D1A27_01050) 4188..4883 - 696 WP_254910806 RepA family replication protein -
D1A27_RS29025 5389..5640 - 252 WP_113394424 hypothetical protein -
D1A27_RS01055 (D1A27_01055) 5652..6185 - 534 WP_032440594 phospholipase D family protein -
D1A27_RS01060 (D1A27_01060) 6231..8423 - 2193 WP_032440593 F-type conjugative transfer protein TrbC -
D1A27_RS01065 (D1A27_01065) 8425..9516 - 1092 WP_050597336 thioredoxin fold domain-containing protein trbB
D1A27_RS01070 (D1A27_01070) 9527..10945 - 1419 WP_063666705 hypothetical protein trbA
D1A27_RS01075 (D1A27_01075) 11047..11670 - 624 WP_032440592 plasmid IncI1-type surface exclusion protein ExcA -
D1A27_RS01080 (D1A27_01080) 11736..13880 - 2145 WP_032440591 DotA/TraY family protein traY
D1A27_RS01085 (D1A27_01085) 13922..14503 - 582 WP_050597335 conjugal transfer protein TraX -
D1A27_RS01090 (D1A27_01090) 14500..15696 - 1197 WP_032440590 conjugal transfer protein TraW traW
D1A27_RS01095 (D1A27_01095) 15706..18753 - 3048 WP_032440589 conjugal transfer protein traU
D1A27_RS29030 18763..19326 - 564 WP_045325070 hypothetical protein traT
D1A27_RS01105 (D1A27_01105) 19370..19771 - 402 WP_032440588 DUF6750 family protein traR
D1A27_RS01110 (D1A27_01110) 19827..20360 - 534 WP_032440587 conjugal transfer protein TraQ traQ
D1A27_RS01115 (D1A27_01115) 20370..21089 - 720 WP_050597333 conjugal transfer protein TraP traP
D1A27_RS01120 (D1A27_01120) 21096..22436 - 1341 WP_032440586 conjugal transfer protein TraO traO
D1A27_RS01125 (D1A27_01125) 22440..23543 - 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
D1A27_RS01130 (D1A27_01130) 23554..24222 - 669 WP_032440585 DotI/IcmL/TraM family protein traM
D1A27_RS01135 (D1A27_01135) 24294..24647 - 354 WP_032440584 hypothetical protein traL
D1A27_RS29230 24681..27758 - 3078 WP_172583156 LPD7 domain-containing protein -
D1A27_RS01145 (D1A27_01145) 27970..28236 - 267 WP_032440577 IcmT/TraK family protein traK
D1A27_RS01150 (D1A27_01150) 28233..29420 - 1188 WP_077255878 plasmid transfer ATPase TraJ -
D1A27_RS01155 (D1A27_01155) 29398..30216 - 819 WP_032440576 type IV secretory system conjugative DNA transfer family protein traI
D1A27_RS01160 (D1A27_01160) 30213..30677 - 465 WP_225546581 DotD/TraH family lipoprotein -
D1A27_RS01165 (D1A27_01165) 30703..31182 - 480 WP_032440574 lytic transglycosylase domain-containing protein -
D1A27_RS01170 (D1A27_01170) 31914..32141 - 228 WP_032440573 hypothetical protein -
D1A27_RS01175 (D1A27_01175) 32751..33593 + 843 WP_032440572 helix-turn-helix domain-containing protein -
D1A27_RS01180 (D1A27_01180) 33653..34582 + 930 WP_032440581 hypothetical protein -
D1A27_RS01185 (D1A27_01185) 34575..35069 + 495 WP_032440571 molybdopterin-dependent oxidoreductase -

Host bacterium

ID   2738 GenBank   NZ_QWDG01000009
Plasmid name   B15|unnamed P9 Incompatibility group   IncFII
Plasmid size   63966 bp Coordinate of oriT [Strand]   39325..39406 [-]
Host baterium   Klebsiella pneumoniae strain B15

Cargo genes

Drug resistance gene   qnrS1, blaLAP-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -