Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102290
Name   oriT_B15|unnamed in_silico
Organism   Klebsiella pneumoniae strain B15
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_QWDG01000002 (9180..9267 [-], 88 nt)
oriT length   88 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 88 nt

>oriT_B15|unnamed
GGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1756 GenBank   WP_011264053
Name   nikB_D1A27_RS00315_B15|unnamed insolico UniProt ID   A0A767TAZ8
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103882.34 Da        Isoelectric Point: 7.4198

>WP_011264053.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A767TAZ8


Auxiliary protein

ID   693 GenBank   WP_001283947
Name   WP_001283947_B15|unnamed insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1346 GenBank   WP_072107381
Name   trbC_D1A27_RS00320_B15|unnamed insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86883.07 Da        Isoelectric Point: 6.7712

>WP_072107381.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTAKTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 14646..53494

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1A27_RS00320 (D1A27_00320) 12362..14653 - 2292 WP_072107381 F-type conjugative transfer protein TrbC -
D1A27_RS00325 (D1A27_00325) 14646..15716 - 1071 WP_039510685 IncI1-type conjugal transfer protein TrbB trbB
D1A27_RS00330 (D1A27_00330) 15735..16943 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
D1A27_RS00335 (D1A27_00335) 17235..17387 + 153 WP_001331364 Hok/Gef family protein -
D1A27_RS00340 (D1A27_00340) 17459..17710 - 252 WP_001291964 hypothetical protein -
D1A27_RS30215 18211..18306 + 96 WP_001303310 DinQ-like type I toxin DqlB -
D1A27_RS29215 (D1A27_00350) 18371..18508 - 138 WP_047088497 hypothetical protein -
D1A27_RS00355 (D1A27_00355) 18553..19779 - 1227 WP_024186316 RNA-guided endonuclease TnpB family protein -
D1A27_RS00360 (D1A27_00360) 19838..20242 + 405 WP_001175009 IS200/IS605 family transposase -
D1A27_RS00365 (D1A27_00365) 20574..20783 - 210 WP_032178830 hemolysin expression modulator Hha -
D1A27_RS00370 (D1A27_00370) 20881..21543 - 663 WP_000644794 plasmid IncI1-type surface exclusion protein ExcA -
D1A27_RS00375 (D1A27_00375) 21614..23782 - 2169 WP_000698368 DotA/TraY family protein traY
D1A27_RS00380 (D1A27_00380) 23879..24463 - 585 WP_032072591 IncI1-type conjugal transfer protein TraX -
D1A27_RS00385 (D1A27_00385) 24492..25694 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
D1A27_RS00390 (D1A27_00390) 25661..26275 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
D1A27_RS00395 (D1A27_00395) 26275..29319 - 3045 WP_001024782 IncI1-type conjugal transfer protein TraU traU
D1A27_RS00400 (D1A27_00400) 29409..30209 - 801 WP_001164792 IncI1-type conjugal transfer protein TraT traT
D1A27_RS00405 (D1A27_00405) 30193..30381 - 189 WP_001277255 putative conjugal transfer protein TraS -
D1A27_RS00410 (D1A27_00410) 30445..30849 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
D1A27_RS00415 (D1A27_00415) 30900..31427 - 528 WP_001055569 conjugal transfer protein TraQ traQ
D1A27_RS00420 (D1A27_00420) 31427..32131 - 705 WP_023155244 IncI1-type conjugal transfer protein TraP traP
D1A27_RS00425 (D1A27_00425) 32131..33420 - 1290 WP_001271994 conjugal transfer protein TraO traO
D1A27_RS00430 (D1A27_00430) 33423..34406 - 984 WP_021513963 IncI1-type conjugal transfer protein TraN traN
D1A27_RS00435 (D1A27_00435) 34417..35109 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
D1A27_RS00440 (D1A27_00440) 35106..35453 - 348 WP_001055900 conjugal transfer protein traL
D1A27_RS00445 (D1A27_00445) 35471..39238 - 3768 WP_006855639 LPD7 domain-containing protein -
D1A27_RS00450 (D1A27_00450) 39328..39879 - 552 WP_000014584 phospholipase D family protein -
D1A27_RS00455 (D1A27_00455) 39894..40184 - 291 WP_001299214 hypothetical protein traK
D1A27_RS00460 (D1A27_00460) 40181..41329 - 1149 WP_001024976 plasmid transfer ATPase TraJ virB11
D1A27_RS00465 (D1A27_00465) 41326..42144 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
D1A27_RS00470 (D1A27_00470) 42141..42599 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
D1A27_RS00475 (D1A27_00475) 42994..43578 - 585 WP_000977525 histidine phosphatase family protein -
D1A27_RS00480 (D1A27_00480) 43638..44840 - 1203 WP_000976353 conjugal transfer protein TraF -
D1A27_RS00485 (D1A27_00485) 44926..45750 - 825 WP_001690630 conjugal transfer protein TraE traE
D1A27_RS00490 (D1A27_00490) 45901..47055 - 1155 WP_073481693 site-specific integrase -
D1A27_RS29890 (D1A27_00495) 47054..47461 + 408 WP_227642478 prepilin, shufflon protein A -
D1A27_RS30290 (D1A27_00500) 47458..47697 - 240 Protein_55 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
D1A27_RS00505 (D1A27_00505) 47691..49001 - 1311 WP_117355371 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
D1A27_RS00510 (D1A27_00510) 49001..49657 - 657 WP_001193551 A24 family peptidase -
D1A27_RS00515 (D1A27_00515) 49642..50202 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
D1A27_RS00520 (D1A27_00520) 50212..50826 - 615 WP_000908228 type 4 pilus major pilin -
D1A27_RS00525 (D1A27_00525) 50843..51928 - 1086 WP_001208803 type II secretion system F family protein -
D1A27_RS00530 (D1A27_00530) 51941..53494 - 1554 WP_000362204 ATPase, T2SS/T4P/T4SS family virB11
D1A27_RS00535 (D1A27_00535) 53505..53957 - 453 WP_117355372 type IV pilus biogenesis protein PilP -
D1A27_RS00540 (D1A27_00540) 53944..55239 - 1296 WP_117355373 type 4b pilus protein PilO2 -
D1A27_RS00545 (D1A27_00545) 55232..56914 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
D1A27_RS00550 (D1A27_00550) 56928..57365 - 438 WP_000539811 type IV pilus biogenesis protein PilM -
D1A27_RS00555 (D1A27_00555) 57365..58432 - 1068 WP_021513968 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   2734 GenBank   NZ_QWDG01000002
Plasmid name   B15|unnamed Incompatibility group   IncI1
Plasmid size   75138 bp Coordinate of oriT [Strand]   9180..9267 [-]
Host baterium   Klebsiella pneumoniae strain B15

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -