Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102288
Name   oriT_B03|unnamed in_silico
Organism   Klebsiella pneumoniae strain B03
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_NTHT01000084 (12477..12576 [-], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      77..82, 90..95  (AAAAAA..TTTTTT)
 78..83, 90..95  (AAAAAA..TTTTTT)
 79..84, 90..95  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_B03|unnamed
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGAAAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1755 GenBank   WP_012561142
Name   mobF_CK488_RS28165_B03|unnamed insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   692 GenBank   WP_001749975
Name   WP_001749975_B03|unnamed insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   1345 GenBank   WP_000342688
Name   t4cp2_CK488_RS28160_B03|unnamed insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 30690..41019

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CK488_RS28215 26618..27397 + 780 WP_004152394 IS21-like element ISKpn7 family helper ATPase IstB -
CK488_RS28225 27784..28665 + 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
CK488_RS28230 28915..30234 - 1320 WP_004152397 IS1182-like element ISKpn6 family transposase -
CK488_RS29315 30586..30690 - 105 Protein_32 phospholipase D family protein -
CK488_RS28240 30690..31685 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
CK488_RS28245 31727..32887 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
CK488_RS28250 32887..33771 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
CK488_RS28255 33782..34480 - 699 WP_000646594 type IV secretion system protein virB8
CK488_RS29785 34470..34628 - 159 WP_012561180 hypothetical protein -
CK488_RS28265 34699..35739 - 1041 WP_001749958 type IV secretion system protein virB6
CK488_RS28270 35755..35982 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
CK488_RS28275 35990..36703 - 714 WP_001749960 type IV secretion system protein virB5
CK488_RS28280 36721..39321 - 2601 WP_012561149 VirB4 family type IV secretion/conjugal transfer ATPase virb4
CK488_RS28285 39321..39638 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
CK488_RS28290 39689..39982 - 294 WP_001749962 hypothetical protein virB2
CK488_RS28295 39992..40273 - 282 WP_016338364 transcriptional repressor KorA -
CK488_RS28300 40282..41019 - 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
CK488_RS28305 41062..41433 + 372 WP_223174968 H-NS family nucleoid-associated regulatory protein -
CK488_RS28310 41449..41793 + 345 WP_012561155 hypothetical protein -
CK488_RS28315 41790..42104 + 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
CK488_RS28320 42140..42451 + 312 WP_013279382 hypothetical protein -
CK488_RS29655 42507..42767 + 261 WP_016359294 hypothetical protein -
CK488_RS28330 42809..43148 - 340 WP_071813319 IS5/IS1182 family transposase -
CK488_RS28335 43159..44053 - 895 Protein_52 IS5 family transposase -
CK488_RS28340 44073..44384 + 312 WP_071527918 EAL domain-containing protein -
CK488_RS28345 44411..45358 - 948 WP_004152065 sensor domain-containing diguanylate cyclase -

Host bacterium

ID   2732 GenBank   NZ_NTHT01000084
Plasmid name   B03|unnamed Incompatibility group   IncN
Plasmid size   96802 bp Coordinate of oriT [Strand]   12477..12576 [-]
Host baterium   Klebsiella pneumoniae strain B03

Cargo genes

Drug resistance gene   blaKPC-2, tet(A)
Virulence gene   -
Metal resistance gene   fecE, fecD
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -