Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102250 |
| Name | oriT_pB16 P32b |
| Organism | Klebsiella pneumoniae strain B16 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_NTCW01000082 (2091..2150 [-], 60 nt) |
| oriT length | 60 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 60 nt
>oriT_pB16 P32b
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTA
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1731 | GenBank | WP_172412685 |
| Name | Relaxase_CLI87_RS30050_pB16 P32b |
UniProt ID | _ |
| Length | 135 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 135 a.a. Molecular weight: 15312.53 Da Isoelectric Point: 8.3739
>WP_172412685.1 relaxase/mobilization nuclease domain-containing protein, partial [Klebsiella pneumoniae]
MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELIDASPYVKKYTSGVLSFAEADLPPGQR
EKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAW
MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELIDASPYVKKYTSGVLSFAEADLPPGQR
EKLMASFERVLMPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAW
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 674 | GenBank | WP_071599364 |
| Name | WP_071599364_pB16 P32b |
UniProt ID | A0A9Q5XKH5 |
| Length | 113 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 113 a.a. Molecular weight: 12571.54 Da Isoelectric Point: 8.8015
>WP_071599364.1 MULTISPECIES: MobC family plasmid mobilization relaxosome protein [Enterobacterales]
MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTRAGRLPSISPALLRQLAGMGNNLNQI
ARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGPDDDC
MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTRAGRLPSISPALLRQLAGMGNNLNQI
ARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGPDDDC
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 2694 | GenBank | NZ_NTCW01000082 |
| Plasmid name | pB16 P32b | Incompatibility group | Col440I |
| Plasmid size | 2980 bp | Coordinate of oriT [Strand] | 2091..2150 [-] |
| Host baterium | Klebsiella pneumoniae strain B16 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |