Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102233
Name   oriT_Kp1803|unnamed2 in_silico
Organism   Klebsiella pneumoniae strain Kp1803
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_PITN01000044 (31357..31462 [-], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_Kp1803|unnamed2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1723 GenBank   WP_004187323
Name   Relaxase_CWR46_RS25080_Kp1803|unnamed2 insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP

ID   1308 GenBank   WP_004206885
Name   t4cp2_CWR46_RS24960_Kp1803|unnamed2 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1398..28561

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWR46_RS24935 (CWR46_24930) 1..436 - 436 WP_032419546 hypothetical protein -
CWR46_RS24940 (CWR46_24935) 516..899 + 384 WP_019725042 DUF1496 domain-containing protein -
CWR46_RS29230 1004..1174 + 171 WP_234715330 hypothetical protein -
CWR46_RS24950 (CWR46_24945) 1398..2705 + 1308 WP_015059988 hypothetical protein trbA
CWR46_RS24955 (CWR46_24950) 2716..3666 + 951 WP_004206886 DsbC family protein trbB
CWR46_RS24960 (CWR46_24955) 3679..5766 + 2088 WP_004206885 conjugal transfer protein TrbC -
CWR46_RS29360 5817..5912 - 96 WP_004206884 DinQ-like type I toxin DqlB -
CWR46_RS24965 (CWR46_24960) 7135..8190 - 1056 WP_015060006 plasmid replication initiator RepA -
CWR46_RS24970 (CWR46_24965) 8486..8728 - 243 WP_023893611 IncL/M type plasmid replication protein RepC -
CWR46_RS24975 (CWR46_24970) 8797..9450 - 654 WP_015060005 hypothetical protein -
CWR46_RS24980 (CWR46_24975) 9452..11647 - 2196 WP_015062834 DotA/TraY family protein traY
CWR46_RS24985 (CWR46_24980) 11640..12236 - 597 WP_015060003 hypothetical protein -
CWR46_RS24990 (CWR46_24985) 12233..13441 - 1209 WP_011091082 conjugal transfer protein TraW traW
CWR46_RS24995 (CWR46_24990) 13438..16488 - 3051 WP_031942490 plasmid transfer protein traU
CWR46_RS25000 (CWR46_24995) 16485..16979 - 495 WP_011091080 hypothetical protein -
CWR46_RS25005 (CWR46_25000) 17025..17414 - 390 WP_011091078 DUF6750 family protein traR
CWR46_RS25010 (CWR46_25005) 17431..17961 - 531 WP_104011266 conjugal transfer protein TraQ traQ
CWR46_RS25015 (CWR46_25010) 17985..18689 - 705 WP_031942491 conjugal transfer protein TraP traP
CWR46_RS25020 (CWR46_25015) 18701..20050 - 1350 WP_004187474 conjugal transfer protein TraO traO
CWR46_RS25025 (CWR46_25020) 20062..21213 - 1152 WP_004187471 DotH/IcmK family type IV secretion protein traN
CWR46_RS25035 (CWR46_25030) 21222..21935 - 714 WP_004187467 DotI/IcmL family type IV secretion protein traM
CWR46_RS25040 (CWR46_25035) 22003..22413 + 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
CWR46_RS25045 (CWR46_25040) 22442..22597 + 156 WP_172693602 Hha/YmoA family nucleoid-associated regulatory protein -
CWR46_RS25050 (CWR46_25045) 22598..23110 - 513 WP_011091071 hypothetical protein traL
CWR46_RS29235 23076..26333 - 3258 WP_242446732 LPD7 domain-containing protein -
CWR46_RS25060 (CWR46_25055) 26358..26618 - 261 WP_004187310 IcmT/TraK family protein traK
CWR46_RS25065 (CWR46_25060) 26608..27771 - 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
CWR46_RS25070 (CWR46_25065) 27782..28561 - 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
CWR46_RS25075 (CWR46_25070) 28558..29058 - 501 WP_004187320 DotD/TraH family lipoprotein -
CWR46_RS25080 (CWR46_25075) 29072..31051 - 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
CWR46_RS29240 31038..31565 - 528 WP_172740549 plasmid mobilization protein MobA -
CWR46_RS25090 (CWR46_25085) 31600..31995 + 396 WP_019725163 hypothetical protein -
CWR46_RS25095 (CWR46_25090) 32497..33033 - 537 WP_004187332 hypothetical protein -
CWR46_RS25100 (CWR46_25095) 33166..33477 - 312 WP_004187333 hypothetical protein -

Host bacterium

ID   2677 GenBank   NZ_PITN01000044
Plasmid name   Kp1803|unnamed2 Incompatibility group   IncL/M
Plasmid size   40351 bp Coordinate of oriT [Strand]   31357..31462 [-]
Host baterium   Klebsiella pneumoniae strain Kp1803

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -