Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102189
Name   oriT_FWSEC0532|unnamed1 in_silico
Organism   Escherichia coli strain FWSEC0532
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRRZ01000361 (3594..3717 [+], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      92..99, 113..120  (ATAATGTA..TACATTAT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_FWSEC0532|unnamed1
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTATTTGAATCATTAACTTATGTCTTAAATAATGTATTTTAATTTATTTTACATTATAAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1695 GenBank   WP_136759859
Name   mobF_C9300_RS26645_FWSEC0532|unnamed1 insolico UniProt ID   _
Length   582 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 582 a.a.        Molecular weight: 63779.74 Da        Isoelectric Point: 6.5930

>WP_136759859.1 MobF family relaxase, partial [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAESRMQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTTGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLISDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYAR

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(10-283)


  Protein structure



No available structure.




Auxiliary protein

ID   667 GenBank   WP_001151555
Name   WP_001151555_FWSEC0532|unnamed1 insolico UniProt ID   _
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14461.36 Da        Isoelectric Point: 4.5221

>WP_001151555.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Escherichia]
MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLELGLRVYEAQMERKESAFNQAEFNKVL
LECAIKTQSTVAKILGIESLSPHVSGNPKFEYANMVEDIRDKVSSEMERFFPENDEE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure



No available structure.



ID   668 GenBank   WP_001254381
Name   WP_001254381_FWSEC0532|unnamed1 insolico UniProt ID   _
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9037.10 Da        Isoelectric Point: 9.9851

>WP_001254381.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Escherichia]
MRRRNAKAGTSRTISVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRYPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-61)


  Protein structure



No available structure.




T4CP

ID   1296 GenBank   WP_001064271
Name   traC_C9300_RS26510_FWSEC0532|unnamed1 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99434.22 Da        Isoelectric Point: 5.9665

>WP_001064271.1 MULTISPECIES: type IV secretion system protein TraC [Escherichia]
MNNPLEAVTQAVNSLVTALKLPDESSKANEILGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANETIVEALDHMLRTKLPRGIPLCIHLMSSQLVGERIKYGLREFSWSGEQAERFNAITRAYYMKAAE
TLFPLPEGLNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLVKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRQEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraG P-loop domain (P-loop_TraG)

(468-771)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.



ID   1297 GenBank   WP_000009375
Name   traD_C9300_RS26630_FWSEC0532|unnamed1 insolico UniProt ID   _
Length   714 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 714 a.a.        Molecular weight: 81137.72 Da        Isoelectric Point: 5.1669

>WP_000009375.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Escherichia]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKLSWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATIVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDLNPEMENRLSAVLAAREAEGRQMASLFEPEVVSGEDVTQAEQPQQPVSSVINDK
KSDAGVSVPAGGIAQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQREQNPDIQQQMQRREEVNINV
HRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3022..32049

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9300_RS26430 (C9300_26450) 243..1247 - 1005 WP_024166184 IS110 family transposase -
C9300_RS26435 (C9300_26455) 1498..1785 + 288 WP_000107535 hypothetical protein -
C9300_RS26440 (C9300_26460) 1904..2725 + 822 Protein_2 DUF932 domain-containing protein -
C9300_RS26445 (C9300_26465) 3022..3669 - 648 WP_000614283 transglycosylase SLT domain-containing protein virB1
C9300_RS26450 (C9300_26470) 3955..4338 + 384 WP_001151555 conjugal transfer relaxosome DNA-binding protein TraM -
C9300_RS26455 (C9300_26475) 4532..5218 + 687 WP_000332479 PAS domain-containing protein -
C9300_RS26460 (C9300_26480) 5312..5539 + 228 WP_001254381 conjugal transfer relaxosome protein TraY -
C9300_RS26465 (C9300_26485) 5573..5932 + 360 WP_000340277 type IV conjugative transfer system pilin TraA -
C9300_RS26470 (C9300_26490) 5947..6258 + 312 WP_000012121 type IV conjugative transfer system protein TraL traL
C9300_RS26475 (C9300_26495) 6280..6846 + 567 WP_000399757 type IV conjugative transfer system protein TraE traE
C9300_RS26480 (C9300_26500) 6833..7561 + 729 WP_001355329 type-F conjugative transfer system secretin TraK traK
C9300_RS26485 (C9300_26505) 7561..8991 + 1431 WP_136759858 F-type conjugal transfer pilus assembly protein TraB traB
C9300_RS26490 (C9300_26510) 8981..9568 + 588 WP_000002892 conjugal transfer pilus-stabilizing protein TraP -
C9300_RS26495 (C9300_26515) 9555..9875 + 321 WP_001057269 DUF2689 domain-containing protein -
C9300_RS26500 (C9300_26520) 9872..10387 + 516 WP_000802746 type IV conjugative transfer system lipoprotein TraV traV
C9300_RS26505 (C9300_26525) 10522..10743 + 222 WP_001278688 conjugal transfer protein TraR -
C9300_RS26510 (C9300_26530) 10903..13530 + 2628 WP_001064271 type IV secretion system protein TraC virb4
C9300_RS26515 (C9300_26535) 13527..13913 + 387 WP_000214089 type-F conjugative transfer system protein TrbI -
C9300_RS26520 (C9300_26540) 13910..14542 + 633 WP_000650119 type-F conjugative transfer system protein TraW traW
C9300_RS26525 (C9300_26545) 14539..15531 + 993 WP_001355328 conjugal transfer pilus assembly protein TraU traU
C9300_RS28815 15807..16326 + 520 Protein_20 hypothetical protein -
C9300_RS26535 (C9300_26555) 16353..16991 + 639 WP_000087541 type-F conjugative transfer system pilin assembly protein TrbC trbC
C9300_RS26540 (C9300_26560) 16988..17368 + 381 WP_000009645 hypothetical protein -
C9300_RS27925 17357..17719 - 363 WP_169072562 hypothetical protein -
C9300_RS26550 (C9300_26570) 17646..19508 + 1863 WP_249819127 type-F conjugative transfer system mating-pair stabilization protein TraN traN
C9300_RS26555 (C9300_26575) 19522..19779 + 258 WP_000864356 conjugal transfer protein TrbE -
C9300_RS26560 (C9300_26580) 19772..20515 + 744 WP_001030399 type-F conjugative transfer system pilin assembly protein TraF traF
C9300_RS26565 (C9300_26585) 20560..20865 + 306 WP_236922840 conjugal transfer protein TrbA -
C9300_RS26570 (C9300_26590) 20846..21181 - 336 WP_000415768 hypothetical protein -
C9300_RS26575 (C9300_26595) 21262..21546 + 285 WP_000624195 type-F conjugative transfer system pilin chaperone TraQ -
C9300_RS26580 (C9300_26600) 21533..22069 + 537 WP_000059854 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
C9300_RS26585 (C9300_26605) 22059..22373 + 315 WP_001244895 P-type conjugative transfer protein TrbJ -
C9300_RS26590 (C9300_26610) 22327..22719 + 393 WP_000138366 F-type conjugal transfer protein TrbF -
C9300_RS26595 (C9300_26615) 22706..24079 + 1374 WP_001137366 conjugal transfer pilus assembly protein TraH traH
C9300_RS26600 (C9300_26620) 24076..26919 + 2844 WP_001006995 conjugal transfer mating-pair stabilization protein TraG traG
C9300_RS26605 (C9300_26625) 26935..27438 + 504 WP_000605506 hypothetical protein -
C9300_RS26610 (C9300_26630) 27470..28201 + 732 WP_001364068 conjugal transfer complement resistance protein TraT -
C9300_RS26615 (C9300_26635) 28405..28884 + 480 WP_001355324 hypothetical protein -
C9300_RS26625 (C9300_26645) 29119..29849 + 731 Protein_38 hypothetical protein -
C9300_RS26630 (C9300_26650) 29905..32049 + 2145 WP_000009375 type IV conjugative transfer system coupling protein TraD virb4
C9300_RS26635 (C9300_26655) 32058..32456 - 399 WP_000911316 type II toxin-antitoxin system VapC family toxin -
C9300_RS26640 (C9300_26660) 32456..32683 - 228 WP_000450526 toxin-antitoxin system antitoxin VapB -
C9300_RS26645 (C9300_26665) 32765..34511 + 1747 WP_136759859 MobF family relaxase -

Host bacterium

ID   2633 GenBank   NZ_RRRZ01000361
Plasmid name   FWSEC0532|unnamed1 Incompatibility group   -
Plasmid size   34511 bp Coordinate of oriT [Strand]   3594..3717 [+]
Host baterium   Escherichia coli strain FWSEC0532

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -