Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102104 |
| Name | oriT_FWSEC0539|unnamed5  |
| Organism | Escherichia coli strain FWSEC0539 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_RRSF01000263 (10470..10593 [-], 124 nt) |
| oriT length | 124 nt |
| IRs (inverted repeats) | 92..99, 113..120 (ATAATGTA..TACATTAT) 90..95, 107..112 (AAATAA..TTATTT) 39..46, 49..56 (GCAAAAAC..GTTTTTGC) 3..10, 15..22 (TTGGTGGT..ACCACCAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
>oriT_FWSEC0539|unnamed5
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTATTTGAATCATTAACTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATAAAA
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTATTTGAATCATTAACTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATAAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1290 | GenBank | WP_001064239 |
| Name | traC_C9306_RS27550_FWSEC0539|unnamed5 |
UniProt ID | _ |
| Length | 875 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 875 a.a. Molecular weight: 99485.47 Da Isoelectric Point: 6.6265
>WP_001064239.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGWR
LLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGM
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGWR
LLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGM
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 426..11165
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C9306_RS27545 (C9306_27570) | 43..429 | - | 387 | WP_000214082 | type-F conjugative transfer system protein TrbI | - |
| C9306_RS27550 (C9306_27575) | 426..3053 | - | 2628 | WP_001064239 | type IV secretion system protein TraC | virb4 |
| C9306_RS27555 (C9306_27580) | 3213..3434 | - | 222 | WP_001278978 | conjugal transfer protein TraR | - |
| C9306_RS27560 (C9306_27585) | 3569..4084 | - | 516 | WP_000809893 | type IV conjugative transfer system lipoprotein TraV | traV |
| C9306_RS27565 (C9306_27590) | 4081..4332 | - | 252 | WP_001038341 | conjugal transfer protein TrbG | - |
| C9306_RS27570 (C9306_27595) | 4325..4645 | - | 321 | WP_001057302 | conjugal transfer protein TrbD | virb4 |
| C9306_RS27575 (C9306_27600) | 4632..5216 | - | 585 | WP_000002795 | conjugal transfer pilus-stabilizing protein TraP | - |
| C9306_RS27580 (C9306_27605) | 5206..6633 | - | 1428 | WP_000146638 | F-type conjugal transfer pilus assembly protein TraB | traB |
| C9306_RS27585 (C9306_27610) | 6633..7361 | - | 729 | WP_001230787 | type-F conjugative transfer system secretin TraK | traK |
| C9306_RS27590 (C9306_27615) | 7348..7914 | - | 567 | WP_000399804 | type IV conjugative transfer system protein TraE | traE |
| C9306_RS27595 (C9306_27620) | 7936..8247 | - | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
| C9306_RS27600 (C9306_27625) | 8252..8614 | - | 363 | WP_136818358 | type IV conjugative transfer system pilin TraA | - |
| C9306_RS27605 (C9306_27630) | 8648..8875 | - | 228 | WP_001254388 | conjugal transfer relaxosome protein TraY | - |
| C9306_RS27610 (C9306_27635) | 8969..9655 | - | 687 | WP_000332487 | PAS domain-containing protein | - |
| C9306_RS27615 (C9306_27640) | 9849..10232 | - | 384 | WP_001151564 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| C9306_RS27620 (C9306_27645) | 10518..11165 | + | 648 | WP_000614282 | transglycosylase SLT domain-containing protein | virB1 |
| C9306_RS27625 (C9306_27650) | 11462..12283 | - | 822 | WP_001234469 | DUF932 domain-containing protein | - |
| C9306_RS27630 (C9306_27655) | 12404..12691 | - | 288 | WP_000107537 | hypothetical protein | - |
| C9306_RS31895 | 12992..13128 | + | 137 | Protein_18 | hypothetical protein | - |
Host bacterium
| ID | 2548 | GenBank | NZ_RRSF01000263 |
| Plasmid name | FWSEC0539|unnamed5 | Incompatibility group | - |
| Plasmid size | 13128 bp | Coordinate of oriT [Strand] | 10470..10593 [-] |
| Host baterium | Escherichia coli strain FWSEC0539 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |