Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102101
Name   oriT_ARPG-380|unnamed15 in_silico
Organism   Klebsiella pneumoniae strain ARPG-380
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_NBON01000061 (22983..23133 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_ARPG-380|unnamed15
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1659 GenBank   WP_001326173
Name   mobH_B7D70_RS27150_ARPG-380|unnamed15 insolico UniProt ID   F5BPR1
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB F5BPR1


T4CP

ID   1288 GenBank   WP_000178857
Name   traD_B7D70_RS27155_ARPG-380|unnamed15 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.



ID   1289 GenBank   WP_000637384
Name   traC_B7D70_RS27230_ARPG-380|unnamed15 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92604.86 Da        Isoelectric Point: 5.9216

>WP_000637384.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 21067..46210

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D70_RS27140 (B7D70_27050) 17098..17331 - 234 WP_001191890 hypothetical protein -
B7D70_RS27145 (B7D70_27055) 17313..17930 - 618 WP_001249395 hypothetical protein -
B7D70_RS27150 (B7D70_27060) 18098..21070 + 2973 WP_001326173 MobH family relaxase -
B7D70_RS27155 (B7D70_27065) 21067..22932 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
B7D70_RS27160 (B7D70_27070) 22982..23527 + 546 WP_000228720 hypothetical protein -
B7D70_RS27165 (B7D70_27075) 23484..24113 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
B7D70_RS27170 (B7D70_27080) 24123..24569 + 447 WP_000122507 hypothetical protein -
B7D70_RS27175 (B7D70_27085) 24579..24956 + 378 WP_000869297 hypothetical protein -
B7D70_RS27180 (B7D70_27090) 24956..25618 + 663 WP_001231464 hypothetical protein -
B7D70_RS29680 25799..25942 + 144 WP_001275801 hypothetical protein -
B7D70_RS27185 (B7D70_27095) 25954..26319 + 366 WP_001052530 hypothetical protein -
B7D70_RS27190 (B7D70_27100) 26464..26745 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
B7D70_RS27195 (B7D70_27105) 26742..27368 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
B7D70_RS27200 (B7D70_27110) 27352..28269 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
B7D70_RS27205 (B7D70_27115) 28269..29582 + 1314 WP_024131605 TraB/VirB10 family protein traB
B7D70_RS27210 (B7D70_27120) 29579..30157 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
B7D70_RS27215 (B7D70_27125) 30161..30553 + 393 WP_000479535 TraA family conjugative transfer protein -
B7D70_RS27220 (B7D70_27130) 30754..36240 + 5487 WP_000606835 Ig-like domain-containing protein -
B7D70_RS27225 (B7D70_27135) 36389..37096 + 708 WP_001259346 DsbC family protein -
B7D70_RS27230 (B7D70_27140) 37093..39540 + 2448 WP_000637384 type IV secretion system protein TraC virb4
B7D70_RS27235 (B7D70_27145) 39555..39872 + 318 WP_000351984 hypothetical protein -
B7D70_RS27240 (B7D70_27150) 39869..40399 + 531 WP_001010740 S26 family signal peptidase -
B7D70_RS27245 (B7D70_27155) 40362..41627 + 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
B7D70_RS27250 (B7D70_27160) 41624..42295 + 672 WP_000575345 EAL domain-containing protein -
B7D70_RS27255 (B7D70_27165) 42295..43299 + 1005 WP_005507569 TraU family protein traU
B7D70_RS27260 (B7D70_27170) 43415..46210 + 2796 WP_001256484 conjugal transfer mating pair stabilization protein TraN traN
B7D70_RS27265 (B7D70_27175) 46249..47109 - 861 WP_000709517 hypothetical protein -
B7D70_RS27270 (B7D70_27180) 47232..47873 - 642 WP_000796664 hypothetical protein -
B7D70_RS27275 (B7D70_27185) 48168..48488 + 321 WP_000547566 hypothetical protein -
B7D70_RS27280 (B7D70_27190) 48792..48977 + 186 WP_001186917 hypothetical protein -
B7D70_RS27285 (B7D70_27195) 49197..50165 + 969 WP_000085160 AAA family ATPase -
B7D70_RS27290 (B7D70_27200) 50176..51084 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   2545 GenBank   NZ_NBON01000061
Plasmid name   ARPG-380|unnamed15 Incompatibility group   -
Plasmid size   75445 bp Coordinate of oriT [Strand]   22983..23133 [+]
Host baterium   Klebsiella pneumoniae strain ARPG-380

Cargo genes

Drug resistance gene   erm(42)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -