Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102069
Name   oriT_KP41|unnamed1 KP41_40 in_silico
Organism   Klebsiella pneumoniae strain KP41
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MOLX01000040 (3065..3165 [-], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_KP41|unnamed1 KP41_40
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1652 GenBank   WP_058659946
Name   mobF_BLJ97_RS25865_KP41|unnamed1 KP41_40 insolico UniProt ID   _
Length   1080 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1080 a.a.        Molecular weight: 120433.62 Da        Isoelectric Point: 6.5483

>WP_058659946.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLIGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLSQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure



No available structure.




Auxiliary protein

ID   661 GenBank   WP_001749975
Name   WP_001749975_KP41|unnamed1 KP41_40 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   1280 GenBank   WP_001749976
Name   t4cp2_BLJ97_RS25860_KP41|unnamed1 KP41_40 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57679.73 Da        Isoelectric Point: 9.5788

>WP_001749976.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Gammaproteobacteria]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVSARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELRDI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 3732..23580

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLJ97_RS25825 (BLJ97_25835) 118..297 + 180 WP_000214483 protein CcgAI -
BLJ97_RS25830 (BLJ97_25840) 352..672 + 321 WP_001749971 protein CcgAII -
BLJ97_RS25835 (BLJ97_25845) 783..1127 - 345 WP_000999874 hypothetical protein -
BLJ97_RS25840 (BLJ97_25850) 1309..1677 - 369 WP_001749972 plasmid stabilization protein StbC -
BLJ97_RS25845 (BLJ97_25855) 1679..2395 - 717 WP_001749973 StbB family protein -
BLJ97_RS32100 (BLJ97_25860) 2404..2823 - 420 WP_001749974 plasmid stabilization protein StbA -
BLJ97_RS25855 (BLJ97_25865) 3314..3730 + 417 WP_001749975 hypothetical protein -
BLJ97_RS25860 (BLJ97_25870) 3732..5261 + 1530 WP_001749976 type IV secretion system DNA-binding domain-containing protein virb4
BLJ97_RS25865 (BLJ97_25875) 5261..8503 + 3243 WP_058659946 MobF family relaxase -
BLJ97_RS25870 (BLJ97_25880) 8503..8676 + 174 WP_001749978 hypothetical protein -
BLJ97_RS25875 (BLJ97_25885) 8908..9150 + 243 WP_000844627 transposase -
BLJ97_RS25880 (BLJ97_25890) 9182..9832 - 651 WP_000164043 tetracycline resistance transcriptional repressor TetR(A) -
BLJ97_RS25885 (BLJ97_25895) 9938..11137 + 1200 WP_012579085 tetracycline efflux MFS transporter Tet(A) -
BLJ97_RS25890 (BLJ97_25900) 11169..11822 - 654 Protein_13 EamA family transporter -
BLJ97_RS32320 12096..12548 + 453 WP_001749956 DUF6710 family protein -
BLJ97_RS25905 (BLJ97_25915) 12722..13255 - 534 WP_000792636 phospholipase D family protein -
BLJ97_RS25910 (BLJ97_25920) 13255..14250 - 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
BLJ97_RS25915 (BLJ97_25925) 14292..15452 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
BLJ97_RS25920 (BLJ97_25930) 15452..16336 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
BLJ97_RS25925 (BLJ97_25935) 16347..17045 - 699 WP_000646594 type IV secretion system protein virB8
BLJ97_RS32325 17035..17193 - 159 WP_012561180 hypothetical protein -
BLJ97_RS25930 (BLJ97_25940) 17264..18304 - 1041 WP_001749958 type IV secretion system protein virB6
BLJ97_RS25935 (BLJ97_25945) 18320..18547 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
BLJ97_RS25940 (BLJ97_25950) 18555..19268 - 714 WP_001749960 type IV secretion system protein virB5
BLJ97_RS25945 (BLJ97_25955) 19286..21886 - 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
BLJ97_RS25950 (BLJ97_25960) 21886..22203 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
BLJ97_RS25955 (BLJ97_25965) 22253..22546 - 294 WP_001749962 hypothetical protein virB2
BLJ97_RS25960 (BLJ97_25970) 22556..22837 - 282 WP_000440698 transcriptional repressor KorA -
BLJ97_RS25965 (BLJ97_25975) 22846..23580 - 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
BLJ97_RS25970 (BLJ97_25980) 23623..23994 + 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
BLJ97_RS25975 (BLJ97_25985) 24010..24354 + 345 WP_001749964 hypothetical protein -
BLJ97_RS25980 (BLJ97_25990) 24351..24665 + 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
BLJ97_RS25985 (BLJ97_25995) 24779..25012 + 234 WP_001191790 hypothetical protein -
BLJ97_RS25990 (BLJ97_26000) 25062..25709 + 648 WP_015344958 restriction endonuclease -
BLJ97_RS30070 25714..25920 + 207 WP_001749967 hypothetical protein -
BLJ97_RS25995 (BLJ97_26005) 25931..26203 - 273 Protein_35 IS1 family transposase -
BLJ97_RS26000 (BLJ97_26010) 26302..27735 + 1434 WP_001288432 DNA cytosine methyltransferase -

Host bacterium

ID   2513 GenBank   NZ_MOLX01000040
Plasmid name   KP41|unnamed1 KP41_40 Incompatibility group   -
Plasmid size   35402 bp Coordinate of oriT [Strand]   3065..3165 [-]
Host baterium   Klebsiella pneumoniae strain KP41

Cargo genes

Drug resistance gene   tet(A), sul1, qacE, aadA16, dfrA27, ARR-3, aac(6')-Ib-cr
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -